pKLAC1-malE
At a Glance
<protect>
| Type |
Control |
|---|---|
| Origin of Replication |
pMB1 |
| Copy # | |
| Markers |
Ampicillin, Acetamidase |
| Link to Sequence | |
| Features |
α-mating factor |
| Accession Number |
|
| edit table |
</protect>
Notes
pKLAC1-malE is a backbone integrative expression vector that directs high-level expression of recombinant protein from the yeast Kluyveromyces lactis. It carries the E.coli malE gene, encoding the maltose binding protein. pKLAC1-malE can also be used to test plasmid transformation, cell culture, and protein production procedures. [1]
History
A standard plasmid purification procedure was used to derive pKLAC1-malE from E. coli strain ER2268.
Sources
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Colussi, P.A. and Taron, C.H., unpublished observations.
Sequence
1 AAGCTTGAAA AAAATGAAAT TCTCTACTAT ATTAGCCGCA TCTACTGCTT
51 TAATTTCCGT TGTTATGGCT GCTCCAGTTT CTACCGAAAC TGACATCGAC
101 GATCTTCCAA TATCGGTTCC AGAAGAAGCC TTGATTGGAT TCATTGACTT
151 AACCGGGGAT GAAGTTTCCT TGTTGCCTGT TAATAACGGA ACCCACACTG
201 GTATTCTATT CTTAAACACC ACCATCGCTG AAGCTGCTTT CGCTGACAAG
251 GATGATCTCG AGAAAAGAAT GAAAACTGAA GAAGGTAAAC TGGTAATCTG
301 GATTAACGGC GATAAAGGCT ATAACGGTCT CGCTGAAGTC GGTAAGAAAT
351 TCGAGAAAGA TACCGGAATT AAAGTCACCG TTGAGCATCC GGATAAACTG
401 GAAGAGAAAT TCCCACAGGT TGCGGCAACT GGCGATGGCC CTGACATTAT
451 CTTCTGGGCA CACGACCGCT TTGGTGGCTA CGCTCAATCT GGCCTGTTGG
501 CTGAAATCAC CCCGGACAAA GCGTTCCAGG ACAAGCTGTA TCCGTTTACC
551 TGGGATGCCG TACGTTACAA CGGCAAGCTG ATTGCTTACC CGATCGCTGT
601 TGAAGCGTTA TCGCTGATTT ATAACAAAGA TCTGCTGCCG AACCCGCCAA
651 AAACCTGGGA AGAGATCCCG GCGCTGGATA AAGAACTGAA AGCGAAAGGT
701 AAGAGCGCGC TGATGTTCAA CCTGCAAGAA CCGTACTTCA CCTGGCCGCT
751 GATTGCTGCT GACGGGGGTT ATGCGTTCAA GTATGAAAAC GGCAAGTACG
801 ACATTAAAGA CGTGGGCGTG GATAACGCTG GCGCGAAAGC GGGTCTGACC
851 TTCCTGGTTG ACCTGATTAA AAACAAACAC ATGAATGCAG ACACCGATTA
901 CTCCATCGCA GAAGCTGCCT TTAATAAAGG CGAAACAGCG ATGACCATCA
951 ACGGCCCGTG GGCATGGTCC AACATCGACA CCAGCAAAGT GAATTATGGT
1001 GTAACGGTAC TGCCGACCTT CAAGGGTCAA CCATCCAAAC CGTTCGTTGG
1051 CGTGCTGAGC GCAGGTATTA ACGCCGCCAG TCCGAACAAA GAGCTGGCGA
1101 AAGAGTTCCT CGAAAACTAT CTGCTGACTG ATGAAGGTCT GGAAGCGGTT
1151 AATAAAGACA AACCGCTGGG TGCCGTAGCG CTGAAGTCTT ACGAGGAAGA
1201 GTTGGCGAAA GATCCACGTA TTGCCGCCAC CATGGAAAAC GCCCAGAAAG
1251 GTGAAATCAT GCCGAACATC CCGCAGATGT CCGCTTTCTG GTATGCCGTG
1301 CGTACTGCGG TGATCAACGC CGCCAGCGGT CGTCAGACTG TCGATGAAGC
1351 CCTGAAAGAC GCGCAGACTA ATTCGAGCTC GTAAGCGGCC GCTTAATTAA
1401 GGCCTTGAAT CGAGAATTTA TACTTAGATA AGTATGTACT TACAGGTATA
1451 TTTCTATGAG ATACTGATGT ATACATGCAT GATAATATTT AAACGGTTAT
1501 TAGTGCCGAT TGTCTTGTGC GATAATGACG TTCCTATCAA AGCAATACAC
1551 TTACCACCTA TTACATGGGC CAAGAAAATA TTTTCGAACT TGTTTAGAAT
1601 ATTAGCACAG AGTATATGAT GATATCCGTT AGATTATGCA TGATTCATTC
1651 CTACAACTTT TTCGTAGCAT AAGGATTAAT TACTTGGATG CCAATAAAAA
1701 AAAAAAACAT CGAGAAAATT TCAGCATGCT CAGAAACAAT TGCAGTGTAT
1751 CAAAGTAAAA AAAAGATTTT CACTACATGT TCCTTTTGAA GAAAGAAAAT
1801 CATGGAACAT TAGATTTACA AAAATTTAAC CACCGCTGAT TAACGATTAG
1851 ACCGTTAAGC GCACAACAGG TTATTAGTAC AGAGAAAGCA TTCTGTGGTG
1901 TTGCCCCGGA CTTTCTTTTG CGACATAGGT AAATCGAATA CCATCATACT
1951 ATCTTTTCCA ATGACTCCCT AAAGAAAGAC TCTTCTTCGA TGTTGTATAC
2001 GTTGGAGCAT AGGGCAAGAA TTGTGGCTTG AGATCATCCT TTTGTTGTTT
2051 CCGGGTGTAC AATATGGACT TCCTCTTTTC TGGCAACCAA ACCCATACAT
2101 CGGGATTCCT ATAATACCTT CGTTGGTCTC CCTAACATGT AGGTGGCGGA
2151 GGGGAGATAT ACAATAGAAC AGATACCAGA CAAGACATAA TGGGCTAAAC
2201 AAGACTACAC CAATTACACT GCCTCATTGA TGGTGGTACA TAACGAACTA
2251 ATACTGTAGC CCTAGACTTG ATAGCCATCA TCATATCGAA GTTTCACTAC
2301 CCTTTTTCCA TTTGCCATCT ATTGAAGTAA TAATAGGCGC ATGCAACTTC
2351 TTTTCTTTTT TTTTCTTTTC TCTCTCCCCC GTTGTTGTCT CACCATATCC
2401 GCAATGACAA AAAAATGATG GAAGACACTA AAGGAAAAAA TTAACGACAA
2451 AGACAGCACC AACAGATGTC GTTGTTCCAG AGCTGATGAG GGGTATCTCG
2501 AAGCACACGA AACTTTTTCC TTCCTTCATT CACGCACACT ACTCTCTAAT
2551 GAGCAACGGT ATACGGCCTT CCTTCCAGTT ACTTGAATTT GAAATAAAAA
2601 AAAGTTTGCT GTCTTGCTAT CAAGTATAAA TAGACCTGCA ATTATTAATC
2651 TTTTGTTTCC TCGTCATTGT TCTCGTTCCC TTTCTTCCTT GTTTCTTTTT
2701 CTGCACAATA TTTCAAGCTA TACCAAGCAT ACAATCAAGG AATTCCGGAT
2751 CCGCCACCAT GCCTCAATCC TGGGAAGAAC TGGCCGCTGA TAAGCGCGCC
2801 CGCCTCGCAA AAACCATCCC TGATGAATGG AAAGTCCAGA CGCTGCCTGC
2851 GGAAGACAGC GTTATTGATT TCCCAAAGAA ATCGGGGATC CTTTCAGAGG
2901 CCGAACTGAA GATCACAGAG GCTTCCGCTG CGGATCTTGT GTCCAAGCTG
2951 GCGGCCGGAG AGTTGACCTC GGTGGAAGTT ACGCTAGCAT TCTGTAAACG
3001 GGCAGCAATC GCCCAGCAGT TAACAAACTG CGCCCACGAG TTCTTCCCTG
3051 ACGCCGCTCT CGCGCAGGCA AGGGAACTCG ATGAATACTA CGCAAAGCAC
3101 AAGAGACCCG TTGGTCCACT CCATGGCCTC CCCATCTCTC TCAAAGACCA
3151 GCTTCGAGTC AAGGGCTACG AAACATCAAT GGGCTACATC TCATGGCTAA
3201 ACAAGTACGA CGAAGGGGAC TCGGTTCTGA CAACCATGCT CCGCAAAGCC
3251 GGTGCCGTCT TCTACGTCAA GACCTCTGTC CCGCAGACCC TGATGGTCTG
3301 CGAGACAGTC AACAACATCA TCGGGCGCAC CGTCAACCCA CGCAACAAGA
3351 ACTGGTCGTG CGGCGGCAGT TCTGGTGGTG AGGGTGCGAT CGTTGGGATT
3401 CGTGGTGGCG TCATCGGTGT AGGAACGGAT ATCGGTGGCT CGATTCGAGT
3451 GCCGGCCGCG TTCAACTTCC TGTACGGTCT AAGGCCGAGT CATGGGCGGC
3501 TGCCGTATGC AAAGATGGCG AACAGCATGG AGGGTCAGGA GACGGTGCAC
3551 AGCGTTGTCG GGCCGATTAC GCACTCTGTT GAGGACCTCC GCCTCTTCAC
3601 CAAATCCGTC CTCGGTCAGG AGCCATGGAA ATACGACTCC AAGGTCATCC
3651 CCATGCCCTG GCGCCAGTCC GAGTCGGACA TTATTGCCTC CAAGATCAAG
3701 AACGGCGGGC TCAATATCGG CTACTACAAC TTCGACGGCA ATGTCCTTCC
3751 ACACCCTCCT ATCCTGCGCG GCGTGGAAAC TACCGTCGCC GCACTCGCCA
3801 AAGCCGGTCA CACCGTGACC CCGTGGACGC CATACAAGCA CGATTTCGGC
3851 CACGATCTCA TCTCCCATAT CTACGCGGCT GACGGCAGCG CCGACGTAAT
3901 GCGCGATATC AGTGCATCCG GCGAGCCGGC GATTCCAAAT ATCAAAGACC
3951 TACTGAACCC GAACATCAAA GCTGTTAACA TGAACGAGCT CTGGGACACG
4001 CATCTCCAGA AGTGGAATTA CCAGATGGAG TACCTTGAGA AATGGCGGGA
4051 GGCTGAAGAA AAGGCCGGGA AGGAACTGGA CGCCATCATC GCGCCGATTA
4101 CGCCTACCGC TGCGGTACGG CATGACCAGT TCCGGTACTA TGGGTATGCC
4151 TCTGTGATCA ACCTGCTGGA TTTCACGAGC GTGGTTGTTC CGGTTACCTT
4201 TGCGGATAAG AACATCGATA AGAAGAATGA GAGTTTCAAG GCGGTTAGTG
4251 AGCTTGATGC CCTCGTGCAG GAAGAGTATG ATCCGGAGGC GTACCATGGG
4301 GCACCGGTTG CAGTGCAGGT TATCGGACGG AGACTCAGTG AAGAGAGGAC
4351 GTTGGCGATT GCAGAGGAAG TGGGGAAGTT GCTGGGAAAT GTGGTGACTC
4401 CATAGCCCGG GGGGGGCTCG ATCCCCTCGC GAGTTGGTTC AGCTGCTGCC
4451 TGAGGCTGGA CGACCTCGCG GAGTTCTACC GGCAGTGCAA ATCCGTCGGC
4501 ATCCAGGAAA CCAGCAGCGG CTATCCGCGC ATCCATGCCC CCGAACTGCA
4551 GGAGTGGGGA GGCACGATGG CCGCTTTGGT CGATCTAGAT TACGTGGAAG
4601 AAAGGTAGTA AAAGTAGTAG TATAAGTAGT AAAAAGAGGT AAAAAGAGAA
4651 AACCGGCTAC ATACTAGAGA AGCACGTACA CAAAAACTCA TAGGCACTTC
4701 ATCATACGAC AGTTTCTTGA TGCATTATAA TAGTGTATTA GATATTTTCA
4751 GAAATATGCA TAGAACCTCC TCTTGCCTTT ACTTTTTATA CATAGAACAT
4801 TGGCAGATTT ACTTACACTA CTTTGTTTCT ACGCCATTTC TTTTGTTTTC
4851 AACACTTAGA CAAGTTGTTG AGAACCGGAC TACTAAAAAG CAATGTTCCC
4901 ACTGAAAATC ATGTACCTGC AGGATAATAA CCCCCTAATT CTGCATCGAT
4951 CCAGTATGTT TTTTTTTCTC TACTCATTTT TACCTGAAGA TAGAGCTTCT
5001 AAAACAAAAA AAATCAGTGA TTACATGCAT ATTGTGTGTT CTAGTAACCA
5051 AAGGAAAGGA ACAGATAGAT AAAATTCCGA GACTGTCAAA TTAGGTTTTT
5101 TTCTTTTTTT TTGGCGGGAG TCAGTGGGCC GAAATATGTT CTTGGCCTAG
5151 AACTTAATCT GGTTTGATCA TGCCAATACT TGCCTGAGTG CCCGACTTTT
5201 TGCCCACCCT CTTGCCTTCT GTCATCCTTC AAAACCCACC TGTTTTCCAG
5251 CCGTATCTTC GCTCGCATCT ACACATACTG TGCCATATCT TGTGTGTAGC
5301 CGGACGTGAC TATGACCAAA AACAAACAAG GAGAACTGTT CGCCGATTTG
5351 TAACACTCCT GCATCCATCC AAGTGGGTAT GCGCTATGCA ATGTTAAGCT
5401 AGGTCAGGTC AGACCAGGTC CAAGGACAGC AACTTGACTG TATGCAACCT
5451 TTACCATCTT TGCACAGAAC ATACTTGTAG CTAGCTAGTT ACACTTATGG
5501 ACCGAAAAGG CACCCCACCA TGTCTGTCCG GCTTTAGAGT ACGGCCGCAG
5551 ACCGCTGATT TGCCTTGCCA AGCAGTAGTC ACAATGCATC GCATGAGCAC
5601 ACGGGCACGG GCACGGGCAC AGGAACCATT GGCAAAAATA CCAGATACAC
5651 TATACCGACG TATATCAAGC CCAAGTTTAA AATTCCTAAA TTTCCGCGGC
5701 TACTTTTCAA TTCCCTATAG TGAGTCGTAT TAAATTCGTA ATCATGTCAT
5751 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
5801 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA
5851 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC
5901 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
5951 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
6001 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
6051 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
6101 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
6151 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
6201 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
6251 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
6301 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
6351 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
6401 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
6451 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
6501 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
6551 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
6601 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
6651 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
6701 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
6751 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
6801 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
6851 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
6901 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
6951 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
7001 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
7051 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
7101 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
7151 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
7201 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
7251 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
7301 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
7351 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
7401 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
7451 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
7501 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
7551 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
7601 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
7651 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
7701 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
7751 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
7801 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
7851 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC
7901 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG
7951 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT
8001 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT
8051 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC
8101 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT
8151 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA
8201 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT
8251 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC
8301 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA
8351 ATTTCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG
8401 CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG
8451 GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA
8501 CGACGGCCAG TGCCAAGCTC CCGCGGGGAT CGACTCATAA AATAGTAACC
8551 TTCTAATGCG TATCTATTGA CTACCAACCA TTAGTGTGGT TGCAGAAGGC
8601 GGAATTCTCC CTTCTTCGAA TTCAGCTTGC TTTTTCATTT TTTATTTTCC
8651 ATTTTTCAGT TTTTGTTTGT GTCGAATTTA GCCAGTTGCT TCTCCAAGAT
8701 GAAAAAAACC CCTGCGCAGT TTCTGTGCTG CAAGATCCTA ATCGACTTTT
8751 CCACCCCCCA CAAAAGTAAA TGTTCTTTTG TTACATTCGC GTGGGTAGCT
8801 AGCTCCCCGA ATCTTCAAAG GACTTAGGGA CTGCACTACA TCAGAGTGTG
8851 TTCACCTGGT TTGCTGCCTG GTTTGAAAGA AAAGAGCAGG GAACTCGCGG
8901 GTTCCCGGCG AATAATCATG CGATAGTCCT TTGGCCTTCC AAGTCGCATG
8951 TAGAGTAGAC AACAGACAGG GAGGGCAGGA AGGATCTTTC ACTGAGATCC
9001 TGTATCTTGT TGGGTAAGTC GGATGAAAGG GGAATCGTAT GAGATTGGAG
9051 AGGATGCGGA AGAGGTAACG CCTTTTGTTA ACTTGTTTAA TTATTATGGG
9101 GCAGGCGAGA GGGGGAGGAA TGTATGTGTG TGAGGCGGGC GAGACGGAGC
9151 CATCCAGGCC AGGTAGAAAT AGAGAAAGCC GAATGTTAGA CAATATGGCA
9201 GCGTAGTAGA GTAGGTAGGT AGGCAAGTAC TGCTAGCAAA GAGGAGAAGG
9251 GTAAGCTCAC TCTTCGCATT CCACACCGTT AGTGTGTCAG TTTGAACAAA
9301 AAAACAATCA TCATACCAAT TGATGGACTG TGGACTGGCT TTTGGAACGG
9351 CTTTTCGGAC TGCGATTATT CGTGAGGAAT CAAGGTAGGA ATTTGGTCAT
9401 ATTTACGGAC AACAGTGGGT GATTCCCATA TGGAGTAGGA AAACGAGATC
9451 ATGGTATCCT CAGATATGTT GCGGAATTCT GTTCACCGCA AAGTTCAGGG
9501 TGCTCTGGTG GGTTTCGGTT GGTCTTTGCT TTGCTTCTCC CTTGTCTTGC
9551 ATGTTAATAA TAGCCTAGCC TGTGAGCCGA AACTTAGGGT AGGCTTAGTG
9601 TTGGAACGTA CATATGTATC ACGTTGACTT GGTTTAACCA GGCGACCTGG
9651 TAGCCAGCCA TACCCACACA CGTTTTTTGT ATCTTCAGTA TAGTTGTGAA
9701 AAGTGTAGCG GAAATTTGTG GTCCGAGCAA CAGCGTCTTT TTCTAGTAGT
9751 GCGGTCGGTT ACTTGGTTGA CATTGGTATT TGGACTTTGT TGCTACACCA
9801 TTCACTACTT GAAGTCGAGT GTGAAGGGTA TGATTTCTAG TGGTGAACAC
9851 CTTTAGTTAC GTAATGTTTT CATTGCTGTT TTACTTGAGA TTTCGATTGA
9901 GAAAAAGGTA TTTAATAGCT CGAATCAATG TGAGAACAGA GAGAAGATGT
9951 TCTTCCCTAA CTCGAAAGGT ATATGAGGCT TGTGTTTCTT AGGAGAATTA
10001 TTATTCTTTT GTTATGTTGC GCTTGTAGTT GGAAAAGGTG AAGAGACAAA
10051 AGCTGGAATT GTGAGCGGAT AACAAGCTCA ACACTTGAAA TTTAGGAAAG
10101 AGCAGAATTT GGCAAAAAAA ATAAAAAAAA AATAAACACA CATACTCATC
10151 GAG
