pGL2-Promoter
At a Glance
pGL2-Promoter, image provided by Promega
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
f1 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
Multiple cloning region |
| Accession Number | |
| edit table |
</protect>
Notes
The plasmid that contains that pGL2-Promoter Vector also contains a luciferase gene. The pGL-2 Promoter is located upstream from this gene. In the promoter-luc transcriptional unit DNA fragments can be inserted. These contain putative enhancer elements which can be inserted upstream or downstream from the promoter-luc unit.
The pGL2-Promoter along with other pGL2 luciferase reporter vectors are factors that could potentially regulate mammalian gene expression. These vectors provide a basis for quantitative analysis for these contributing factors. These factors include cis-acting or trans-acting. An example of the two factors would be promoters and enhancers for cis-acting and various DNA-binding factors for the trans-acting factors. Using the the firefly (Photinus pyralis) luciferase contained in the pGL2 vectors the transciptional activity in transfected cells can be monitored. The pGL2 vectors also contain features that are able to aid in the characterization and mutagenisis of the putative regulatory sequences.
History
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Sources
References
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Sequence
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA 51 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 101 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT 151 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT 201 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGGCATT 251 CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG 301 GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA 351 GGCTATGAAG AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG 401 CACATATCGA GGTGAACATC ACGTACGCGG AATACTTCGA AATGTCCGTT 451 CGGTTGGCAG AAGCTATGAA ACGATATGGG CTGAATACAA ATCACAGAAT 501 CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG GTGTTGGGCG 551 CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA 601 CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT 651 TTCCAAAAAG GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA 701 TAATCCAGAA AATTATTATC ATGGATTCTA AAACGGATTA CCAGGGATTT 751 CAGTCGATGT ACACGTTCGT CACATCTCAT CTACCTCCCG GTTTTAATGA 801 ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA ATTGCACTGA 851 TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG 901 CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG 951 CAATCAAATC ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC 1001 ACGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGATTTCGA 1051 GTCGTCTTAA TGTATAGATT TGAAGAAGAG CTGTTTTTAC GATCCCTTCA 1101 GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA TTTTCATTCT 1151 TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA 1201 ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC 1251 AAAACGCTTC CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA 1301 CTACATCAGC TATTCTGATT ACACCCGAGG GGGATGATAA ACCGGGCGCG 1351 GTCGGTAAAG TTGTTCCATT TTTTGAAGCG AAGGTTGTGG ATCTGGATAC 1401 CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT GTCAGAGGAC 1451 CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG 1501 ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA 1551 AGACGAACAC TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA 1601 AAGGATATCA GGTGGCCCCC GCTGAATTGG AATCGATATT GTTACAACAC 1651 CCCAACATCT TCGACGCGGG CGTGGCAGGT CTTCCCGACG ATGACGCCGG 1701 TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG ACGATGACGG 1751 AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG 1801 TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG 1851 AAAACTCGAC GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG 1901 GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA TGACGAAATT 1951 CTTAGCTATT GTAATACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT 2001 TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG 2051 TGATAATAAG CGGATGAATG GCAGAAATTC GCCGGATCTT TGTGAAGGAA 2101 CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA 2151 AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC 2201 TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA 2251 TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA 2301 AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA 2351 CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA 2401 GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC 2451 TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA 2501 AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT 2551 AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC 2601 TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA 2651 AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCAT 2701 AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC 2751 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT 2801 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC 2851 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 2901 CCAAACTCAT CAATGTATCT TATGATGTCT GGATCCGTCG ACCGATGCCC 2951 TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC 3001 TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC 3051 AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC 3101 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 3151 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 3201 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 3251 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 3301 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 3351 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 3401 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 3451 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 3501 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 3551 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 3601 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 3651 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 3701 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 3751 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 3801 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 3851 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 3901 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 3951 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 4001 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 4051 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 4101 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA 4151 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 4201 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT 4251 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 4301 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 4351 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA 4401 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT 4451 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 4501 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG 4551 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 4601 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 4651 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC 4701 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC 4751 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 4801 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG 4851 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 4901 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 4951 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 5001 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG 5051 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 5101 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 5151 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC 5201 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 5251 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 5301 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT 5351 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 5401 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA 5451 ATTTGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT 5501 GCGGGCCTCT TCGCTATTAC GCCAGCCCAA GCTACCATGA TAAGTAAGTA 5551 ATATTAAGGT ACGTGGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT 5601 CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT 5651 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC 5701 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT 5751 CATCAATGTA TCTTATGGTA CTGTAACTGA GCTAACATAA
