pGL2-Control

From EcoliWiki
Jump to: navigation, search

At a Glance

pGL2-Control, Image made with PlasMapper

<protect>

Type

Cloning

Origin of Replication

f1

Copy #
Markers

Ampicillin

Link to Sequence

NCBI

Features

SV40 Promoter
Luciferase gene (luc)
GLprimer2 binding site
SV40 late poly(A) signal
SV40 Enhancer
RVprimer4 binding site
ColE1-derived plasmid replication origin
lactamase (Ampr) gene
GLprimer1 binding site

Accession Number

X65324

</protect>

Notes

The pGL2-Control Vector contains the SV40 promoter and enhancer sequences, resulting in strong luc expression in many types of mammalian cells. This plasmid is useful in monitoring transfection efficiency in general and is a convenient internal standard for promoter and enhancer activities expressed by pGL2 recombinants.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Promega

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

[1] [2]

Sequence

   1  CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTGCATCTCA
  51  ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
 101  ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
 151  TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
 201  AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTGGCATT
 251  CCGGTACTGT TGGTAAAATG GAAGACGCCA AAAACATAAA GAAAGGCCCG
 301  GCGCCATTCT ATCCTCTAGA GGATGGAACC GCTGGAGAGC AACTGCATAA
 351  GGCTATGAAG AGATACGCCC TGGTTCCTGG AACAATTGCT TTTACAGATG
 401  CACATATCGA GGTGAACATC ACGTACGCGG AATACTTCGA AATGTCCGTT
 451  CGGTTGGCAG AAGCTATGAA ACGATATGGG CTGAATACAA ATCACAGAAT
 501  CGTCGTATGC AGTGAAAACT CTCTTCAATT CTTTATGCCG GTGTTGGGCG
 551  CGTTATTTAT CGGAGTTGCA GTTGCGCCCG CGAACGACAT TTATAATGAA
 601  CGTGAATTGC TCAACAGTAT GAACATTTCG CAGCCTACCG TAGTGTTTGT
 651  TTCCAAAAAG GGGTTGCAAA AAATTTTGAA CGTGCAAAAA AAATTACCAA
 701  TAATCCAGAA AATTATTATC ATGGATTCTA AAACGGATTA CCAGGGATTT
 751  CAGTCGATGT ACACGTTCGT CACATCTCAT CTACCTCCCG GTTTTAATGA
 801  ATACGATTTT GTACCAGAGT CCTTTGATCG TGACAAAACA ATTGCACTGA
 851  TAATGAATTC CTCTGGATCT ACTGGGTTAC CTAAGGGTGT GGCCCTTCCG
 901  CATAGAACTG CCTGCGTCAG ATTCTCGCAT GCCAGAGATC CTATTTTTGG
 951  CAATCAAATC ATTCCGGATA CTGCGATTTT AAGTGTTGTT CCATTCCATC
1001  ACGGTTTTGG AATGTTTACT ACACTCGGAT ATTTGATATG TGGATTTCGA
1051  GTCGTCTTAA TGTATAGATT TGAAGAAGAG CTGTTTTTAC GATCCCTTCA
1101  GGATTACAAA ATTCAAAGTG CGTTGCTAGT ACCAACCCTA TTTTCATTCT
1151  TCGCCAAAAG CACTCTGATT GACAAATACG ATTTATCTAA TTTACACGAA
1201  ATTGCTTCTG GGGGCGCACC TCTTTCGAAA GAAGTCGGGG AAGCGGTTGC
1251  AAAACGCTTC CATCTTCCAG GGATACGACA AGGATATGGG CTCACTGAGA
1301  CTACATCAGC TATTCTGATT ACACCCGAGG GGGATGATAA ACCGGGCGCG
1351  GTCGGTAAAG TTGTTCCATT TTTTGAAGCG AAGGTTGTGG ATCTGGATAC
1401  CGGGAAAACG CTGGGCGTTA ATCAGAGAGG CGAATTATGT GTCAGAGGAC
1451  CTATGATTAT GTCCGGTTAT GTAAACAATC CGGAAGCGAC CAACGCCTTG
1501  ATTGACAAGG ATGGATGGCT ACATTCTGGA GACATAGCTT ACTGGGACGA
1551  AGACGAACAC TTCTTCATAG TTGACCGCTT GAAGTCTTTA ATTAAATACA
1601  AAGGATATCA GGTGGCCCCC GCTGAATTGG AATCGATATT GTTACAACAC
1651  CCCAACATCT TCGACGCGGG CGTGGCAGGT CTTCCCGACG ATGACGCCGG
1701  TGAACTTCCC GCCGCCGTTG TTGTTTTGGA GCACGGAAAG ACGATGACGG
1751  AAAAAGAGAT CGTGGATTAC GTCGCCAGTC AAGTAACAAC CGCGAAAAAG
1801  TTGCGCGGAG GAGTTGTGTT TGTGGACGAA GTACCGAAAG GTCTTACCGG
1851  AAAACTCGAC GCAAGAAAAA TCAGAGAGAT CCTCATAAAG GCCAAGAAGG
1901  GCGGAAAGTC CAAATTGTAA AATGTAACTG TATTCAGCGA TGACGAAATT
1951  CTTAGCTATT GTAATACTGC GATGAGTGGC AGGGCGGGGC GTAATTTTTT
2001  TAAGGCAGTT ATTGGTGCCC TTAAACGCCT GGTTGCTACG CCTGAATAAG
2051  TGATAATAAG CGGATGAATG GCAGAAATTC GCCGGATCTT TGTGAAGGAA
2101  CCTTACTTCT GTGGTGTGAC ATAATTGGAC AAACTACCTA CAGAGATTTA
2151  AAGCTCTAAG GTAAATATAA AATTTTTAAG TGTATAATGT GTTAAACTAC
2201  TGATTCTAAT TGTTTGTGTA TTTTAGATTC CAACCTATGG AACTGATGAA
2251  TGGGAGCAGT GGTGGAATGC CTTTAATGAG GAAAACCTGT TTTGCTCAGA
2301  AGAAATGCCA TCTAGTGATG ATGAGGCTAC TGCTGACTCT CAACATTCTA
2351  CTCCTCCAAA AAAGAAGAGA AAGGTAGAAG ACCCCAAGGA CTTTCCTTCA
2401  GAATTGCTAA GTTTTTTGAG TCATGCTGTG TTTAGTAATA GAACTCTTGC
2451  TTGCTTTGCT ATTTACACCA CAAAGGAAAA AGCTGCACTG CTATACAAGA
2501  AAATTATGGA AAAATATTCT GTAACCTTTA TAAGTAGGCA TAACAGTTAT
2551  AATCATAACA TACTGTTTTT TCTTACTCCA CACAGGCATA GAGTGTCTGC
2601  TATTAATAAC TATGCTCAAA AATTGTGTAC CTTTAGCTTT TTAATTTGTA
2651  AAGGGGTTAA TAAGGAATAT TTGATGTATA GTGCCTTGAC TAGAGATCAT
2701  AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC
2751  CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
2801  AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
2851  AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
2901  CCAAACTCAT CAATGTATCT TATCATGTCT GGATCTGAAC CATGGAGCGG
2951  AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG
3001  GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG
3051  CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG
3101  ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC
3151  ACCCTAACTG ACACACATTC CACAGCAGAT CCTCTAGGGA TCCGTCGACC
3201  GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG
3251  GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC
3301  GTAGGACAGG TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
3351  TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG
3401  GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
3451  AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
3501  CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
3551  TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
3601  TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
3651  CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT
3701  AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
3751  GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
3801  GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
3851  GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
3901  TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG
3951  TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT
4001  GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
4051  TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
4101  CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
4151  TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
4201  TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
4251  CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
4301  ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
4351  AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
4401  CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
4451  GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
4501  CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA
4551  ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
4601  TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
4651  AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
4701  CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
4751  ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
4801  TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC
4851  GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
4901  CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
4951  AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
5001  CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
5051  GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
5101  GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA
5151  GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
5201  TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
5251  ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA
5301  CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC
5351  GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC
5401  TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC
5451  TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG
5501  CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA
5551  TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT
5601  ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA
5651  AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC
5701  GCTTACAATT TGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC
5751  GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCCCAAGCT ACCATGATAA
5801  GTAAGTAATA TTAAGGTACG TGGAGGTTTT ACTTGCTTTA AAAAACCTCC
5851  CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
5901  AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC
5951  AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
6001  CCAAACTCAT CAATGTATCT TATGGTACTG TAACTGAGCT AACATAA
  1. Henikoff, S (1987) Unidirectional digestion with exonuclease III in DNA sequence analysis. Meth. Enzymol. 155 156-65 PubMed
  2. Rosenthal, N (1987) Identification of regulatory elements of cloned genes with functional assays. Meth. Enzymol. 152 704-20 PubMed