pGEX-5X-3
At a Glance
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Type |
Cloning |
---|---|
Origin of Replication | |
Copy # | |
Markers |
Ampicillin |
Link to Sequence | |
Features |
tac Promoter |
Accession Number | |
edit table |
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Notes
pGEX5X-3 is a derivative of pGEX-3X and possess a factor Xa recognition site. Requires very mild elution conditions for release of fusion proteins from the affinity matrix, thus minimizing effects on antigenicity and functional activity.
History
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Sources
References
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Sequence
1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg atcgaaggtc gtgggatccc caggaattcc cgggtcgact 961 cgagcggccg catcgtgact gactgacgat ctgcctcgcg cgtttcggtg atgacggtga 1021 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 1081 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat 1141 gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg gcctcgtgat 1201 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac 1261 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 1321 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 1381 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 1441 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 1501 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 1561 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 1621 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 1681 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 1741 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 1801 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 1861 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 1921 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 1981 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 2041 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 2101 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 2161 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 2221 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 2281 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 2341 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 2401 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 2461 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 2521 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 2581 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 2641 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 2701 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 2761 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 2821 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 2881 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 2941 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 3001 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 3061 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 3121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 3181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataaa 3241 ttccgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga 3301 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc 3361 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa 3421 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc 3481 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct 3541 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag 3601 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa 3661 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc 3721 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca 3781 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca 3841 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc 3901 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat 3961 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct 4021 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc 4081 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata 4141 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt 4201 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt 4261 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa 4321 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 4381 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 4441 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 4501 gataacaatt tcacacagga aacagctatg accatgatta cggattcact ggccgtcgtt 4561 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat 4621 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag 4681 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg 4741 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg 4801 cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat tacggtcaat 4861 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat 4921 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttgg aatt