pGEX-5X-3
At a Glance
pGEX-5X-3, Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication | |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
tac Promoter |
| Accession Number | |
| edit table |
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Notes
pGEX5X-3 is a derivative of pGEX-3X and possess a factor Xa recognition site. Requires very mild elution conditions for release of fusion proteins from the affinity matrix, thus minimizing effects on antigenicity and functional activity.
History
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Sources
References
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Sequence
1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatctg atcgaaggtc gtgggatccc caggaattcc cgggtcgact
961 cgagcggccg catcgtgact gactgacgat ctgcctcgcg cgtttcggtg atgacggtga
1021 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
1081 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
1141 gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg gcctcgtgat
1201 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
1261 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
1321 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
1381 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
1441 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
1501 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
1561 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
1621 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
1681 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
1741 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
1801 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
1861 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
1921 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
1981 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
2041 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
2101 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
2161 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
2221 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
2281 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
2341 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
2401 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
2461 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
2521 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
2581 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
2641 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
2701 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
2761 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
2821 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
2881 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
2941 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
3001 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
3061 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
3121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
3181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataaa
3241 ttccgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
3301 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
3361 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
3421 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
3481 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
3541 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
3601 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
3661 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
3721 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
3781 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
3841 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
3901 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
3961 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
4021 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
4081 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
4141 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
4201 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
4261 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
4321 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
4381 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
4441 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
4501 gataacaatt tcacacagga aacagctatg accatgatta cggattcact ggccgtcgtt
4561 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
4621 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
4681 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
4741 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
4801 cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat tacggtcaat
4861 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
4921 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttgg aatt
