pGEX-5X-2

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At a Glance

pGEX-5X-2, Image made with PlasMapper

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Type

Cloning

Origin of Replication
Copy #
Markers

Ampicillin

Link to Sequence

NCBI

Features

tac Promoter
lac Operator
RBS
MCS
lac Iq

Accession Number

U13857

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Notes

pGEX-5X-2 are derivatives of pGEX-3X and possess a factor Xa recognition site.

History

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Sources

GE Healthcare

References

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Sequence

       1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
      61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     901 atcctccaaa atcggatctg atcgaaggtc gtgggatccc cggaattccc gggtcgactc
     961 gagcggccgc atcgtgactg actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa
    1021 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
    1081 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
    1141 acccagtcac gtagcgatag cggagtgtat aattcttgaa gacgaaaggg cctcgtgata
    1201 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
    1261 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
    1321 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
    1381 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
    1441 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
    1501 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
    1561 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
    1621 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
    1681 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
    1741 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
    1801 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
    1861 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
    1921 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
    1981 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
    2041 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
    2101 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
    2161 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    2221 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
    2281 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    2341 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    2401 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    2461 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    2521 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
    2581 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    2641 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    2701 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    2761 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    2821 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    2881 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    2941 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    3001 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    3061 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    3121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    3181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataaat
    3241 tccgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag
    3301 agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc
    3361 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
    3421 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
    3481 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
    3541 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
    3601 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
    3661 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
    3721 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
    3781 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat
    3841 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
    3901 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
    3961 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
    4021 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
    4081 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
    4141 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
    4201 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
    4261 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
    4321 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
    4381 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    4441 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    4501 ataacaattt cacacaggaa acagctatga ccatgattac ggattcactg gccgtcgttt
    4561 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
    4621 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
    4681 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
    4741 aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc
    4801 agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc
    4861 cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg
    4921 aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttgga att