pGEX-5X-2
At a Glance
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Type |
Cloning |
---|---|
Origin of Replication | |
Copy # | |
Markers |
Ampicillin |
Link to Sequence | |
Features |
tac Promoter |
Accession Number | |
edit table |
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Notes
pGEX-5X-2 are derivatives of pGEX-3X and possess a factor Xa recognition site.
History
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Sources
References
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Sequence
1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg atcgaaggtc gtgggatccc cggaattccc gggtcgactc 961 gagcggccgc atcgtgactg actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa 1021 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 1081 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg 1141 acccagtcac gtagcgatag cggagtgtat aattcttgaa gacgaaaggg cctcgtgata 1201 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 1261 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 1321 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 1381 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 1441 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 1501 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 1561 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 1621 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 1681 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 1741 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 1801 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 1861 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 1921 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 1981 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 2041 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 2101 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 2161 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 2221 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 2281 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 2341 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 2401 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 2461 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 2521 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 2581 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 2641 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 2701 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 2761 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 2821 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 2881 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 2941 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 3001 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 3061 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 3121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 3181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataaat 3241 tccgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag 3301 agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc 3361 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa 3421 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca 3481 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg 3541 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc 3601 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat 3661 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc 3721 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag 3781 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat 3841 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg 3901 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata 3961 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg 4021 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca 4081 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac 4141 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt 4201 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg 4261 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat 4321 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 4381 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 4441 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 4501 ataacaattt cacacaggaa acagctatga ccatgattac ggattcactg gccgtcgttt 4561 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 4621 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 4681 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg 4741 aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc 4801 agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc 4861 cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg 4921 aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttgga att