pGEX-5X-1

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At a Glance

pGEX-5X-1, Image made with PlasMapper

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Type

Cloning

Origin of Replication
Copy #
Markers

Ampicillin

Link to Sequence

NCBI

Features

tac Promoter
lac Operator
RBS
MCS
lac Iq

Accession Number

U13856

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Notes

pGEX-5X-1 allows for removal of the GST carrier protein from the fusion protein by enzymatic cleavage with factor Xa. pGEX-5X-1 can directly accept and express cDNA inserts isolated from l gt11 libraries.

History

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Sources

GE Healthcare

References

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Sequence

       1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
      61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     901 atcctccaaa atcggatctg atcgaaggtc gtgggatccc cgaattcccg ggtcgactcg
     961 agcggccgca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa
    1021 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    1081 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
    1141 cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc ctcgtgatac
    1201 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
    1261 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    1321 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    1381 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    1441 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    1501 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    1561 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    1621 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    1681 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    1741 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    1801 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    1861 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    1921 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    1981 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    2041 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    2101 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    2161 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    2221 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    2281 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    2341 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    2401 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    2461 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    2521 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
    2581 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    2641 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    2701 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    2761 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    2821 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    2881 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    2941 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    3001 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    3061 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    3121 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    3181 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataaatt
    3241 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
    3301 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
    3361 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
    3421 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
    3481 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
    3541 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
    3601 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
    3661 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
    3721 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
    3781 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
    3841 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
    3901 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
    3961 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
    4021 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
    4081 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
    4141 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
    4201 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
    4261 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
    4321 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    4381 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    4441 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    4501 taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt
    4561 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    4621 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
    4681 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga
    4741 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca
    4801 gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc
    4861 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga
    4921 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttggaa tt