pGEX-5X-1
At a Glance
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Type |
Cloning |
---|---|
Origin of Replication | |
Copy # | |
Markers |
Ampicillin |
Link to Sequence | |
Features |
tac Promoter |
Accession Number | |
edit table |
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Notes
pGEX-5X-1 allows for removal of the GST carrier protein from the fusion protein by enzymatic cleavage with factor Xa. pGEX-5X-1 can directly accept and express cDNA inserts isolated from l gt11 libraries.
History
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Sources
References
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Sequence
1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg atcgaaggtc gtgggatccc cgaattcccg ggtcgactcg 961 agcggccgca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa 1021 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 1081 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga 1141 cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc ctcgtgatac 1201 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 1261 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 1321 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 1381 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 1441 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 1501 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 1561 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 1621 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 1681 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 1741 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 1801 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 1861 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 1921 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 1981 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 2041 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 2101 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 2161 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 2221 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 2281 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 2341 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 2401 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 2461 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 2521 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 2581 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 2641 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 2701 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 2761 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 2821 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 2881 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 2941 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 3001 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 3061 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 3121 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 3181 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataaatt 3241 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 3301 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 3361 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 3421 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 3481 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 3541 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 3601 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 3661 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 3721 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 3781 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 3841 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 3901 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 3961 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 4021 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 4081 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 4141 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 4201 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 4261 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 4321 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 4381 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 4441 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 4501 taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt 4561 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 4621 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 4681 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga 4741 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca 4801 gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc 4861 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga 4921 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttggaa tt