pGEX-4T-1
At a Glance
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Type |
Cloning |
---|---|
Origin of Replication | |
Copy # | |
Markers |
Ampicillin |
Link to Sequence | |
Features |
tac Promoter |
Accession Number | |
edit table |
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Notes
pGEX-4T-1 is derived from pGEX-2T and contains a thrombin recognition site. pGEX-4T-1 can directly accept and express cDNA inserts isolated from l gt11 libraries.
History
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Sources
References
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Sequence
1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccccgga attcccgggt cgactcgagc 961 ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc 1021 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 1081 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc 1141 agtcacgtag cgatagcgga gtgtataatt cttgaagacg aaagggcctc gtgatacgcc 1201 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 1261 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 1321 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 1381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 1441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 1501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 1561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg 1621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 1681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 1741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 1801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 1861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 1921 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 1981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 2041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 2101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 2161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 2221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 2281 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 2341 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 2401 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 2461 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 2521 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 2581 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 2641 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 2701 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 2761 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 2821 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 2881 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 2941 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 3001 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 3061 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 3121 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 3181 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataaattccg 3241 acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc 3301 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg 3361 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc 3421 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac 3481 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg 3541 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg 3601 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc 3661 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg 3721 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac 3781 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg 3841 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc 3901 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg 3961 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg 4021 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc 4081 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg 4141 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc 4201 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg 4261 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc 4321 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 4381 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 4441 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 4501 caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca 4561 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 4621 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 4681 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag 4741 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat 4801 gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc 4861 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag 4921 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttggaatt