pG5luc
At a Glance
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
f1 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
five GAL4 binding sites |
| Accession Number | |
| edit table |
</protect>
Notes
The pG5luc vector is part of a two-hybrid system. This two-hybrid system in particular is called the checkmate mammalian two-hybrid system and is extremely powerful when detecting protein:protein interactions in vivo. The main feature of the hybrid system is the modular domains found in some transcription factors. These domains are the DNA-binding domain and a transcriptional activation domain. The DNA-binding domain binds to specific DNA sequences and the transciptional activation domain interacts with the basal transcriptional machinery. RNA polymerase II complexes assembly is promoted at the TATA box and increases transcription when a transcriptional activation domain is in association with a DNA-binding domain. The Checkmate Mammalian two-hybrid system recieves the DNA-binding domain and the transcriptional activation domain from different and separate plasmids. They are closely associated when one protein fused to a DNA-binding domain interacts with a second protein fused to a transcriptional activation domain. The interaction between the two proteins results in transcription of a reporter gene.
History
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Source
References
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Sequence
1 GGTACCGAGT TTCTAGACGG AGTACTGTCC TCCGAGCGGA GTACTGTCCT 51 CCGACTCGAG CGGAGTACTG TCCTCCGATC GGAGTACTGT CCTCCGCGAA 101 TTCCGGAGTA CTGTCCTCCG AAGACGCTAG CGGGGGGCTA TAAAAGGGGG 151 TGGGGGCGTT CGTCCTCACT CTAGATCTGC GATCTAAGTA AGCTTGGCAT 201 TCCGGTACTG TTGGTAAAGC CACCATGGAA GACGCCAAAA ACATAAAGAA 251 AGGCCCGGCG CCATTCTATC CGCTGGAAGA TGGAACCGCT GGAGAGCAAC 301 TGCATAAGGC TATGAAGAGA TACGCCCTGG TTCCTGGAAC AATTGCTTTT 351 ACAGATGCAC ATATCGAGGT GGACATCACT TACGCTGAGT ACTTCGAAAT 401 GTCCGTTCGG TTGGCAGAAG CTATGAAACG ATATGGGCTG AATACAAATC 451 ACAGAATCGT CGTATGCAGT GAAAACTCTC TTCAATTCTT TATGCCGGTG 501 TTGGGCGCGT TATTTATCGG AGTTGCAGTT GCGCCCGCGA ACGACATTTA 551 TAATGAACGT GAATTGCTCA ACAGTATGGG CATTTCGCAG CCTACCGTGG 601 TGTTCGTTTC CAAAAAGGGG TTGCAAAAAA TTTTGAACGT GCAAAAAAAG 651 CTCCCAATCA TCCAAAAAAT TATTATCATG GATTCTAAAA CGGATTACCA 701 GGGATTTCAG TCGATGTACA CGTTCGTCAC ATCTCATCTA CCTCCCGGTT 751 TTAATGAATA CGATTTTGTG CCAGAGTCCT TCGATAGGGA CAAGACAATT 801 GCACTGATCA TGAACTCCTC TGGATCTACT GGTCTGCCTA AAGGTGTCGC 851 TCTGCCTCAT AGAACTGCCT GCGTGAGATT CTCGCATGCC AGAGATCCTA 901 TTTTTGGCAA TCAAATCATT CCGGATACTG CGATTTTAAG TGTTGTTCCA 951 TTCCATCACG GTTTTGGAAT GTTTACTACA CTCGGATATT TGATATGTGG 1001 ATTTCGAGTC GTCTTAATGT ATAGATTTGA AGAAGAGCTG TTTCTGAGGA 1051 GCCTTCAGGA TTACAAGATT CAAAGTGCGC TGCTGGTGCC AACCCTATTC 1101 TCCTTCTTCG CCAAAAGCAC TCTGATTGAC AAATACGATT TATCTAATTT 1151 ACACGAAATT GCTTCTGGTG GCGCTCCCCT CTCTAAGGAA GTCGGGGAAG 1201 CGGTTGCCAA GAGGTTCCAT CTGCCAGGTA TCAGGCAAGG ATATGGGCTC 1251 ACTGAGACTA CATCAGCTAT TCTGATTACA CCCGAGGGGG ATGATAAACC 1301 GGGCGCGGTC GGTAAAGTTG TTCCATTTTT TGAAGCGAAG GTTGTGGATC 1351 TGGATACCGG GAAAACGCTG GGCGTTAATC AAAGAGGCGA ACTGTGTGTG 1401 AGAGGTCCTA TGATTATGTC CGGTTATGTA AACAATCCGG AAGCGACCAA 1451 CGCCTTGATT GACAAGGATG GATGGCTACA TTCTGGAGAC ATAGCTTACT 1501 GGGACGAAGA CGAACACTTC TTCATCGTTG ACCGCCTGAA GTCTCTGATT 1551 AAGTACAAAG GCTATCAGGT GGCTCCCGCT GAATTGGAAT CCATCTTGCT 1601 CCAACACCCC AACATCTTCG ACGCAGGTGT CGCAGGTCTT CCCGACGATG 1651 ACGCCGGTGA ACTTCCCGCC GCCGTTGTTG TTTTGGAGCA CGGAAAGACG 1701 ATGACGGAAA AAGAGATCGT GGATTACGTC GCCAGTCAAG TAACAACCGC 1751 GAAAAAGTTG CGCGGAGGAG TTGTGTTTGT GGACGAAGTA CCGAAAGGTC 1801 TTACCGGAAA ACTCGACGCA AGAAAAATCA GAGAGATCCT CATAAAGGCC 1851 AAGAAGGGCG GAAAGATCGC CGTGTAATTC TAGAGTCGGG GCGGCCGGCC 1901 GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA 1951 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT 2001 GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA 2051 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT 2101 AAAGCAAGTA AAACCTCTAC AAATGTGGTA AAATCGATAA GGATCCGTCG 2151 ACCGATGCCC TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC 2201 GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA 2251 CTCGTAGGAC AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT 2301 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG 2351 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 2401 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 2451 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 2501 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 2551 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 2601 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 2651 CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 2701 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 2751 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 2801 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 2851 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA 2901 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 2951 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 3001 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 3051 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 3101 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 3151 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 3201 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 3251 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA 3301 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 3351 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 3401 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 3451 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 3501 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA 3551 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG 3601 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 3651 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG 3701 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 3751 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 3801 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG 3851 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG 3901 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 3951 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT 4001 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 4051 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 4101 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 4151 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT 4201 GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 4251 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 4301 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA 4351 AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 4401 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 4451 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT 4501 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 4551 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 4601 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT 4651 AACGTTTACA ATTTCCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 4701 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCCCAA GCTACCATGA 4751 TAAGTAAGTA ATATTAAGGT ACGGGAGGTA CTTGGAGCGG CCGCAATAAA 4801 ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGAATCGAT 4851 AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA AACAAACTAG 4901 CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC ATTTCTCTAT 4951 CGATA
