pFN6A (HQ)
At a Glance
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pFN6A (HQ), Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
ColE1 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number | |
| edit table |
</protect>
Notes
This vector is one of two vectors, designed for expressing N-terminal, HisGln (HQ) metal-binding peptide fusion proteins in bacteria and in vitro protein expression systems. It contains a HQ tag that is used to purify protein expression using the MagneHis, MagZ, HisLink 96 or HisLink Protein Purification Systems. This vector is designed specifically for bacterial or in vitro protein putification through the T7 RNA polymerase promoter. Compared to pFN6K (HQ), this vector is ampicilin resistance. In addition, it functions in protein purification via a metal affinity resin.
History
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Sources
References
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Sequence
1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGC AATTTTAACT 51 TTACTAAGGA GAATTCACCA TGAAACATCA ACATCAGCAC CAAGCGATCG 101 CCATGGAATA AGTAAGGAAT CCACATGGCA CAGGTTATCA ACACGTTTGA 151 CGGGGTTGCG GATTATCTTC AGACATATCA TAAGCTACCT GATAATTACA 201 TTACAAAATC AGAAGCACAA GCCCTCGGCT GGGTGGCATC AAAAGGGAAC 251 CTTGCAGACG TCGCTCCGGG GAAAAGCATC GGCGGAGACA TCTTCTCAAA 301 CAGGGAAGGC AAACTCCCGG GCAAAAGCGG ACGAACATGG CGTGAAGCGG 351 ATATTAACTA TACATCAGGC TTCAGAAATT CAGACCGGAT TCTTTACTCA 401 AGCGACTGGC TGATTTACAA AACAACGGAC CATTATCAGA CCTTTACAAA 451 AATCAGATAA TGTTTAAACG AATTCGGGCT CGGTACCCGG GGATCCTCTA 501 GAGTCGACCT GCAGGCATGC AAGCTGATCC GGCTGCTAAC AAAGCCCGAA 551 AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC 601 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC 651 TATATCCGGT TCGCTTGCTG TCCATAAAAC CGCCCAGTCT AGCTATCGCC 701 ATGTAAGCCC ACTGCAAGCT ACCTGCTTTC TCTTTGCGCT TGCGTTTTCC 751 CTTGTCCAGA TAGCCCAGTA GCTGACATTC ATCCGGGGTC AGCACCGTTT 801 CTGCGGACTG GCTTTCTACG TAATGGTTTC TTAGACGTCA GGTGGCACTT 851 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT 901 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA 951 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA 1001 TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 1051 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA 1101 CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 1151 AAGAACGTTT TCCAATGATG AGCACTTTCA AAGTTCTGCT ATGTGGCGCG 1201 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA 1251 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC 1301 TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 1351 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACTATCG GAGGACCGAA 1401 GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 1451 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC 1501 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG 1551 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG 1601 CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 1651 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT 1701 TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 1751 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG 1801 ATAGGTGCCT CACTGATTAA GCATTGGTAA TTCGAAATGA CCGACCAAGC 1851 GACGCCCAAC CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 1901 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 1951 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 2001 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 2051 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 2101 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 2151 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 2201 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 2251 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 2301 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 2351 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 2401 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 2451 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 2501 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 2551 TTACGCGCAG AAAAAAAGGA TTTCAAGAAG ATCCTTTGAT CTTTTCTACG 2601 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 2651 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTATAG TCCGGAAATA 2701 CAGGAACGCA CGCTGGATGG CCCTTCGCTG GGATGGTGAA ACCATGAAAA 2751 ATGGCAGCTT CAGTGGATTA AGTGGGGGTA ATGTGGCCTG TACCCTCTGG 2801 TTGCATAGGT ATTCATACGG TTAAAATTTA TCAGGCGCGA TTGCGGCAGT 2851 TTTTCGGGTG GTTTGTTGCC ATTTTTACCT GTCTGCTGCC GTGATCGCGC 2901 TGAACGCGTT TTAGCGGTGC GTACAATTAA GGGATTATGG TAAATCCACT 2951 TACTGTCTGC CCTCGTAGCC ATCGAGATAA ACCGCAGTAC TCCGGCCACG 3001 ATGCGTCCGG CGTAGAGGAT CGAGATCTTT TCAGCCTGAT ACAGATTAAA 3051 TCAGAACGCA GAAGCGGTCT GATAAAACAG AATTTGCCTG GCGGCAGTAG 3101 CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG AAACGCCGTA 3151 GCGCCGATGG TAGTGTGGGG TCTCCCCATG CGAGAGTAGG GAACTGCCAG 3201 GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA 3251 TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA 3301 GCGGATTTGA ACGTTGCGAA GCAACGGCCC GGAGGGTGGC GGGCAGGACG 3351 CCCGCCATAA ACTGCCAGGC ATCAAATTAA GCAGAAGGCC ATCCTGACGG 3401 ATGGCCTTTT TGCGTTTCTA CAAACTCTTT TGTTTATTTT TCTAAATACA 3451 TTCAAATATG TATCCGCTCA T
