pFN2A (GST)
At a Glance
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pFN2A (GST), Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
ColE1 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 RNA polymerase promoter |
| Accession Number | |
| edit table |
</protect>
Notes
The pFN2A (GST) vector is part of a group of plasmids that are all grouped under the Flexi Vector System. This system is a powerful directional cloning methof for protein-coding sequences. The basis for the method is the two restriction enzymes SgfI(d) and PmeI(c). These are two rare-cutting restriction enzy,es that provide a rapid, efficient way to transfer protein-coding regions back and forth between the Flexi vectors. Using these restriction enzymes elimates the need to resequence. By replacing the DNA sequence of interest by means of the lethal barnase gene contained in each vector, it acts as a positive selection for he successful ligation of the insert.
Use of the Flexi Vectors eliminates the need for appending multiple amino acids to the N- or C-termini of the protein of interest like so many other vectors require. This vector system also does not require the use of an archival entry vector.
History
There are several vectors in this group. They differ by their resistance genes and several other very small differences.
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG AATTTTAACT 51 TTACTAAGGA GAATTTATCA TGTCCCCTAT ACTAGGTTAT TGGAAAATTA 101 AGGGCCTTGT GCAACCCACT CGACTTCTTT TGGAATATCT TGAAGAAAAA 151 TATGAAGAGC ATTTGTATGA GCGCGATGAA GGTGATAAAT GGCGAAACAA 201 AAAGTTTGAA TTGGGTTTGG AGTTTCCCAA TCTTCCTTAT TATATTGATG 251 GTGATGTTAA ATTAACACAG TCTATGGCCA TCATACGTTA TATAGCTGAC 301 AAGCACAACA TGTTGGGTGG TTGTCCAAAA GAGCGTGCAG AGATTTCAAT 351 GCTTGAAGGA GCGGTTTTGG ATATTAGATA CGGTGTTTCG AGAATTGCAT 401 ATAGTAAAGA CTTTGAAACT CTCAAAGTTG ATTTTCTTAG CAAGCTACCT 451 GAAATGCTGA AAATGTTCGA AGATCGTTTA TGTCATAAAA CATATTTGAA 501 TGGTGATCAT GTAACCCATC CTGACTTCAT GTTGTATGAC GCTCTTGATG 551 TTGTTTTATA CATGGACCCA ATGTGCCTGG ATGCGTTCCC AAAATTAGTT 601 TGTTTCAAAA AACGTATTGA AGCTATCCCA CAAATTGATA AGTACTTGAA 651 ATCCAGCAAG TATATAGCAT GGCCTTTGCA GGGCTGGCAA GCCACGTTTG 701 GTGGTGGCGA CCATCCTCCA AAATCCGGAG GTGGTGGCGG AGAAAACCTG 751 TACTTCCAAG CGATCGCCAT GGAATAAGTA AAGGAATCAC ATGGCACAGG 801 TTATCAACAC GTTTGACGGG GTTGCGGATT ATCTTCAGAC ATATCATAAG 851 CTACCTGATA ATTACATTAC AAAATCAGAA GCACAAGCCC TCGGCTGGGT 901 GGCATCAAAA GGGAACCTTG CAGACGTCGC TCCGGGGAAA AGCATCGGCG 951 GAGACATCTT CTCAAACAGG GAAGGCAAAC TCCAGGGCAA AAGCGGACGA 1001 ACATGGCGTG AAGCGGATAT TAACTATACA TCAGGCTTCA GAAATTCAGA 1051 CCGGATTCTT TACTCAAGCG ACTGGCTGAT TTACAAAACA ACGGACCATT 1101 ATCAGACCTT TACAAAAATC AGATAATGTT TAAACGAATT CGAGCTCGGT 1151 ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TGATCCGGCT 1201 GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA 1251 ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT 1301 TGCTGAAAGG AGGAACTATA TCCGGTTCGC TTGCTGTCCA TAAAACCGCC 1351 CAGTCTAGCT ATCGCCATGT AAGCCCACTG CAAGCTACCT GCTTTCTCTT 1401 TGCGCTTGCG TTTTCCCTTG TCCAGATAGC CCAGTAGCTG ACATTCATCC 1451 GGGGTCAGCA CCGTTTCTGC GGACTGGCTT TCTACGTAAT GGTTTCTTAG 1501 ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT 1551 ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT 1601 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT 1651 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 1701 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG 1751 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT 1801 GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTCAAAGT 1851 TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC 1901 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA 1951 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 2001 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA 2051 CTATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT 2101 CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC 2151 AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC 2201 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA 2251 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 2301 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG 2351 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT 2401 ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA 2451 TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAATTCG 2501 AAATGACCGA CCAAGCGACG CCCAACCGGT ATCAGCTCAC TCAAAGGCGG 2551 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 2601 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 2651 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 2701 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 2751 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 2801 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 2851 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 2901 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 2951 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 3001 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 3051 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT 3101 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 3151 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 3201 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATTTC AAGAAGATCC 3251 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 3301 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 3351 TTATAGTCCG GAAATACAGG AACGCACGCT GGATGGCCCT TCGCTGGGAT 3401 GGTGAAACCA TGAAAAATGG CAGCTTCAGT GGATTAAGTG GGGGTAATGT 3451 GGCCTGTACC CTCTGGTTGC ATAGGTATTC ATACGGTTAA AATTTATCAG 3501 GCGCGATTGC GGCAGTTTTT CGGGTGGTTT GTTGCCATTT TTACCTGTCT 3551 GCTGCCGTGA TCGCGCTGAA CGCGTTTTAG CGGTGCGTAC AATTAAGGGA 3601 TTATGGTAAA TCCACTTACT GTCTGCCCTC GTAGCCATCG AGATAAACCG 3651 CAGTACTCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTTTTCAG 3701 CCTGATACAG ATTAAATCAG AACGCAGAAG CGGTCTGATA AAACAGAATT 3751 TGCCTGGCGG CAGTAGCGCG GTGGTCCCAC CTGACCCCAT GCCGAACTCA 3801 GAAGTGAAAC GCCGTAGCGC CGATGGTAGT GTGGGGTCTC CCCATGCGAG 3851 AGTAGGGAAC TGCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC 3901 TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG 3951 GACAAATCCG CCGGGAGCGG ATTTGAACGT TGCGAAGCAA CGGCCCGGAG 4001 GGTGGCGGGC AGGACGCCCG CCATAAACTG CCAGGCATCA AATTAAGCAG 4051 AAGGCCATCC TGACGGATGG CCTTTTTGCG TTTCTACAAA CTCTTTTGTT 4101 TATTTTTCTA AATACATTCA AATATGTATC CGCTCAT
