pFN11A (BIND)

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At a Glance

pFN11A (BIND), Image made with PlasMapper

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Type

Cloning

Origin of Replication

ColE1 derivative

Copy #
Markers

Ampicillin

Link to Sequence

PubMed

Features

CMV promoter
T7 Promoter
SV40 late poly(A)signal
SV40 promoter

Accession Number

DQ487212

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Notes

The pFN10A (ACT) vector is designed to enable confirmation, validation and analysis of supposed interactions between two proteins or domains. This vector possesses the barnase gene, a lethal gene resulting in positive selection for insert ligation, as it is replaced by the DNA fragment of interest. This Flexi vector also expresses the Renilla reniformis luciferase inder the conrol of the SV40 promoter, enabling normalization for variation in transfection efficiency.

History

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Sources

Promega

References

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Sequence

       1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
      61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
     121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
     181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
     421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
     481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
     541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
     661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
     781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
     841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
     901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
     961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
    1021 aggctagagt attaatacga ctcactatag ggctagccag cttgaagcaa gcctcctgaa
    1081 agatgaagct actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca
    1141 agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct
    1201 actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa
    1261 ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga
    1321 ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag
    1381 ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc
    1441 taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag
    1501 gtcaaagaca gttgactgta gcgattccgt cgacaccacc tactccctct ccagcgatcg
    1561 ccatggaata agtaaggaat ccacatggca caggttatca acacgtttga cggggttgcg
    1621 gattatcttc agacatatca taagctacct gataattaca ttacaaaatc agaagcacaa
    1681 gccctcggct gggtggcatc aaaagggaac cttgcagacg tcgctccggg gaaaagcatc
    1741 ggcggagaca tcttctcaaa cagggaaggc aaactcccgg gcaaaagcgg acgaacatgg
    1801 cgtgaagcgg atattaacta tacatcaggc ttcagaaatt cagaccggat tctttactca
    1861 agcgactggc tgatttacaa aacaacggac cattatcaga cctttacaaa aatcagataa
    1921 tgtttaaacg aattcgggct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
    1981 aagctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
    2041 agcaataact agcataaccc cttggggcgg ccgcttcgag cagacatgat aagatacatt
    2101 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
    2161 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
    2221 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt tttaagcaag
    2281 taaaacctct acaaatgtgg taaaatcgaa ttttaacaaa atattaacgc ttacaatttc
    2341 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgcggatct
    2401 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
    2461 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
    2521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
    2581 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
    2641 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
    2701 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    2761 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
    2821 attcttctga cacaacagtc tcgaacttaa ggctagaatt ctggctacag gtaagcgccc
    2881 ctaaaatccc tttgggcaca atgtgtcctg aggggagagg cagcgacctg tagatgggac
    2941 gggggcacta accctcaggt ttggggcttc tgaatgtgag tatcgccatg taagcccagt
    3001 atttggccaa tctcagaaag ctcctggtcc ctggagggat ggagagagaa aaacaaacag
    3061 ctcctggagc agggagagtg ctggcctctt gctctccggc tccctctgtt gccctctggt
    3121 ttctccccag gctcccggac gtcctctagc caccatgact tcgaaagttt atgatccaga
    3181 acaaaggaaa cggatgataa ctggtccgca gtggtgggcc agatgtaaac aaatgaatgt
    3241 tcttgattca tttattaatt attatgattc agaaaaacat gcagaaaatg ctgttatttt
    3301 tttacatggt aacgcggcct cttcttattt atggcgacat gttgtgccac atattgagcc
    3361 agtagcgcgg tgtattatac cagaccttat tggtatgggc aaatcaggca aatctggtaa
    3421 tggttcttat aggttacttg atcattacaa atatcttact gcatggtttg aacttcttaa
    3481 tttaccaaag aagatcattt ttgtcggcca tgattggggt gcttgtttgg catttcatta
    3541 tagctatgag catcaagata agatcaaagc aatagttcac gctgaaagtg tagtagatgt
    3601 gattgaatca tgggatgaat ggcctgatat tgaagaagat attgcgttga tcaaatctga
    3661 agaaggagaa aaaatggttt tggagaataa cttcttcgtg gaaaccatgt tgccatcaaa
    3721 aatcatgaga aagttagaac cagaagaatt tgcagcatat cttgaaccat tcaaagagaa
    3781 aggtgaagtt cgtcgtccaa cattatcatg gcctcgtgaa atcccgttag taaaaggtgg
    3841 taaacctgac gttgtacaaa ttgttaggaa ttataatgct tatctacgtg caagtgatga
    3901 tttaccaaaa atgtttattg aatcggaccc aggattcttt tccaatgcta ttgttgaagg
    3961 tgccaagaag tttcctaata ctgaatttgt caaagtaaaa ggtcttcatt tttcgcaaga
    4021 agatgcacct gatgaaatgg gaaaatatat caaatcgttc gttgagcgag ttctcaaaaa
    4081 tgaacaataa ttctagccct gaataagtga taataagcgg atgaatggca gaaattcgtc
    4141 gaagcgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
    4201 gatagcgata aggatcctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
    4261 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtaa tggtttctta
    4321 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
    4381 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    4441 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
    4501 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
    4561 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
    4621 tgagagtttt cgccccgaag aacgttttcc aatgatgagc actttcaaag ttctgctatg
    4681 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
    4741 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
    4801 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
    4861 acttctgaca actatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
    4921 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
    4981 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
    5041 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
    5101 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
    5161 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    5221 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    5281 cgctgagata ggtgcctcac tgattaagca ttggtaattc gaaatgaccg accaagcgac
    5341 gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    5401 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    5461 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    5521 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    5581 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    5641 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    5701 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    5761 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    5821 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    5881 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
    5941 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    6001 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggattt
    6061 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    6121 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttatagtcc
    6181 ggaaatacag gaacgcacgc tggatggccc ttcgctggga tggtgaaacc atgaaaaatg
    6241 gcagcttcag tggattaagt gggggtaatg tggcctgtac cctctggttg cataggtatt
    6301 catacggtta aaatttatca ggcgcgattg cggcagtttt tcgggtggtt tgttgccatt
    6361 tttacctgtc tgctgccgtg atcgcgctga acgcgtttta gcggtgcgta caattaaggg
    6421 attatggtaa atccacttac tgtctgccct cgtagccatc gagataaacc gcagtactcc
    6481 ggccacgatg cgtccggcgt agaggatcga gatct