pFN11A (BIND)
At a Glance
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pFN11A (BIND), Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
ColE1 derivative |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
CMV promoter |
| Accession Number | |
| edit table |
</protect>
Notes
The pFN10A (ACT) vector is designed to enable confirmation, validation and analysis of supposed interactions between two proteins or domains. This vector possesses the barnase gene, a lethal gene resulting in positive selection for insert ligation, as it is replaced by the DNA fragment of interest. This Flexi vector also expresses the Renilla reniformis luciferase inder the conrol of the SV40 promoter, enabling normalization for variation in transfection efficiency.
History
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Sources
References
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Sequence
1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
1021 aggctagagt attaatacga ctcactatag ggctagccag cttgaagcaa gcctcctgaa
1081 agatgaagct actgtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca
1141 agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct
1201 actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa
1261 ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga
1321 ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag
1381 ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc
1441 taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag
1501 gtcaaagaca gttgactgta gcgattccgt cgacaccacc tactccctct ccagcgatcg
1561 ccatggaata agtaaggaat ccacatggca caggttatca acacgtttga cggggttgcg
1621 gattatcttc agacatatca taagctacct gataattaca ttacaaaatc agaagcacaa
1681 gccctcggct gggtggcatc aaaagggaac cttgcagacg tcgctccggg gaaaagcatc
1741 ggcggagaca tcttctcaaa cagggaaggc aaactcccgg gcaaaagcgg acgaacatgg
1801 cgtgaagcgg atattaacta tacatcaggc ttcagaaatt cagaccggat tctttactca
1861 agcgactggc tgatttacaa aacaacggac cattatcaga cctttacaaa aatcagataa
1921 tgtttaaacg aattcgggct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
1981 aagctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
2041 agcaataact agcataaccc cttggggcgg ccgcttcgag cagacatgat aagatacatt
2101 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
2161 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
2221 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt tttaagcaag
2281 taaaacctct acaaatgtgg taaaatcgaa ttttaacaaa atattaacgc ttacaatttc
2341 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgcggatct
2401 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
2461 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
2521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
2581 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
2641 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
2701 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
2761 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
2821 attcttctga cacaacagtc tcgaacttaa ggctagaatt ctggctacag gtaagcgccc
2881 ctaaaatccc tttgggcaca atgtgtcctg aggggagagg cagcgacctg tagatgggac
2941 gggggcacta accctcaggt ttggggcttc tgaatgtgag tatcgccatg taagcccagt
3001 atttggccaa tctcagaaag ctcctggtcc ctggagggat ggagagagaa aaacaaacag
3061 ctcctggagc agggagagtg ctggcctctt gctctccggc tccctctgtt gccctctggt
3121 ttctccccag gctcccggac gtcctctagc caccatgact tcgaaagttt atgatccaga
3181 acaaaggaaa cggatgataa ctggtccgca gtggtgggcc agatgtaaac aaatgaatgt
3241 tcttgattca tttattaatt attatgattc agaaaaacat gcagaaaatg ctgttatttt
3301 tttacatggt aacgcggcct cttcttattt atggcgacat gttgtgccac atattgagcc
3361 agtagcgcgg tgtattatac cagaccttat tggtatgggc aaatcaggca aatctggtaa
3421 tggttcttat aggttacttg atcattacaa atatcttact gcatggtttg aacttcttaa
3481 tttaccaaag aagatcattt ttgtcggcca tgattggggt gcttgtttgg catttcatta
3541 tagctatgag catcaagata agatcaaagc aatagttcac gctgaaagtg tagtagatgt
3601 gattgaatca tgggatgaat ggcctgatat tgaagaagat attgcgttga tcaaatctga
3661 agaaggagaa aaaatggttt tggagaataa cttcttcgtg gaaaccatgt tgccatcaaa
3721 aatcatgaga aagttagaac cagaagaatt tgcagcatat cttgaaccat tcaaagagaa
3781 aggtgaagtt cgtcgtccaa cattatcatg gcctcgtgaa atcccgttag taaaaggtgg
3841 taaacctgac gttgtacaaa ttgttaggaa ttataatgct tatctacgtg caagtgatga
3901 tttaccaaaa atgtttattg aatcggaccc aggattcttt tccaatgcta ttgttgaagg
3961 tgccaagaag tttcctaata ctgaatttgt caaagtaaaa ggtcttcatt tttcgcaaga
4021 agatgcacct gatgaaatgg gaaaatatat caaatcgttc gttgagcgag ttctcaaaaa
4081 tgaacaataa ttctagccct gaataagtga taataagcgg atgaatggca gaaattcgtc
4141 gaagcgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
4201 gatagcgata aggatcctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
4261 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtaa tggtttctta
4321 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
4381 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
4441 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
4501 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
4561 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
4621 tgagagtttt cgccccgaag aacgttttcc aatgatgagc actttcaaag ttctgctatg
4681 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
4741 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
4801 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
4861 acttctgaca actatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
4921 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
4981 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
5041 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
5101 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
5161 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
5221 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
5281 cgctgagata ggtgcctcac tgattaagca ttggtaattc gaaatgaccg accaagcgac
5341 gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
5401 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
5461 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
5521 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
5581 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
5641 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
5701 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
5761 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
5821 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
5881 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
5941 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
6001 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggattt
6061 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
6121 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttatagtcc
6181 ggaaatacag gaacgcacgc tggatggccc ttcgctggga tggtgaaacc atgaaaaatg
6241 gcagcttcag tggattaagt gggggtaatg tggcctgtac cctctggttg cataggtatt
6301 catacggtta aaatttatca ggcgcgattg cggcagtttt tcgggtggtt tgttgccatt
6361 tttacctgtc tgctgccgtg atcgcgctga acgcgtttta gcggtgcgta caattaaggg
6421 attatggtaa atccacttac tgtctgccct cgtagccatc gagataaacc gcagtactcc
6481 ggccacgatg cgtccggcgt agaggatcga gatct
