pFN10A (ACT)
At a Glance
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pFN10A (ACT), Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
ColE1 derivative |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
CMV promoter |
| Accession Number | |
| edit table |
</protect>
Notes
The pFN10A (ACT) vector is designed to enable confirmation, validation and analysis of supposed interactions between two proteins or domains. This vector possesses the barnase gene, a lethal gene resulting in positive selection for insert ligation, as it is replaced by the DNA fragment of interest.
History
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Sources
References
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Sequence
1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
1021 aggctagagt attaatacga ctcactatag ggctagccag cttgaagcaa gcctcctgaa
1081 agatgaagct actgtcttct atcgaacaag catgcccaaa aaagaagaga aaggtagatg
1141 aatttcctgg gatctctact gctcctccaa ccgatgtcag cctgggcgac gaactccact
1201 tagacggcga ggacgtggcg atggcgcatg ccgacgcgct agacgatttc gatctggaca
1261 tgttggggga cggggattcc ccgggtccgg gatctccagc gattccgtcg acaccaccta
1321 ctccctctcc agcgatcgcc atggaataag taaggaatcc acatggcaca ggttatcaac
1381 acgtttgacg gggttgcgga ttatcttcag acatatcata agctacctga taattacatt
1441 acaaaatcag aagcacaagc cctcggctgg gtggcatcaa aagggaacct tgcagacgtc
1501 gctccgggga aaagcatcgg cggagacatc ttctcaaaca gggaaggcaa actcccgggc
1561 aaaagcggac gaacatggcg tgaagcggat attaactata catcaggctt cagaaattca
1621 gaccggattc tttactcaag cgactggctg atttacaaaa caacggacca ttatcagacc
1681 tttacaaaaa tcagataatg tttaaacgaa ttcgggctcg gtacccgggg atcctctaga
1741 gtcgacctgc aggcatgcaa gctgatccgg ctgctaacaa agcccgaaag gaagctgagt
1801 tggctgctgc caccgctgag caataactag cataacccct tggggcggcc gcttcgagca
1861 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
1921 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
1981 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
2041 gaggtttttt taagcaagta aaacctctac aaatgtggta aaatcgaatt ttaacaaaat
2101 attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
2161 caccgcatac gcggatctgc gcagcaccat ggcctgaaat aacctctgaa agaggaactt
2221 ggttaggtac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
2281 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
2341 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
2401 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
2461 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
2521 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
2581 gcttttgcaa aaagcttgat tcttctgaca caacagtctc gaacttaagg ctagagccac
2641 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
2701 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
2761 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
2821 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
2881 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
2941 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
3001 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
3061 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
3121 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
3181 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
3241 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
3301 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
3361 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
3421 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
3481 ctgccatcac gatggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
3541 tttgtgtgaa tcgatagcga taaggatcct ctttgcgctt gcgttttccc ttgtccagat
3601 agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt
3661 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
3721 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
3781 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
3841 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
3901 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
3961 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcactttcaa
4021 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
4081 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
4141 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
4201 tgcggccaac ttacttctga caactatcgg aggaccgaag gagctaaccg cttttttgca
4261 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
4321 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
4381 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
4441 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
4501 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
4561 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
4621 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaat tcgaaatgac
4681 cgaccaagcg acgcccaacc ggtatcagct cactcaaagg cggtaatacg gttatccaca
4741 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
4801 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
4861 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
4921 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
4981 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
5041 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
5101 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
5161 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
5221 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
5281 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
5341 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
5401 aaaaaaggat ttcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
5461 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
5521 cttttatagt ccggaaatac aggaacgcac gctggatggc ccttcgctgg gatggtgaaa
5581 ccatgaaaaa tggcagcttc agtggattaa gtgggggtaa tgtggcctgt accctctggt
5641 tgcataggta ttcatacggt taaaatttat caggcgcgat tgcggcagtt tttcgggtgg
5701 tttgttgcca tttttacctg tctgctgccg tgatcgcgct gaacgcgttt tagcggtgcg
5761 tacaattaag ggattatggt aaatccactt actgtctgcc ctcgtagcca tcgagataaa
5821 ccgcagtact ccggccacga tgcgtccggc gtagaggatc gagatct
