pFC8K (HaloTag)
At a Glance
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pFC8K (HaloTag), Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
ColE1 derived |
| Copy # | |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
CMV promoter |
| Accession Number | |
| edit table |
</protect>
Notes
- The HaloTag® is a "recombinant protein tag that affords flexibility between protein purification, expression and localization, protein interaction discovery, screening and further functional analysis. The technology is based on the formation of a covalent bond between a protein tag and synthetic ligands."
- The pFC8K(HaloTag) vector is the same as pFC8A(HaloTag) vector with the exception that pFC8K has Kanamycin resistance instead of Ampicillin.
History
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Sources
Promega. Discontinued.
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggaa taagtaagga
1081 atccacatgg cacaggttat caacacgttt gacggggttg cggattatct tcagacatat
1141 cataagctac ctgataatta cattacaaaa tcagaagcac aagccctcgg ctgggtggca
1201 tcaaaaggga accttgcaga cgtcgctccg gggaaaagca tcggcggaga catcttctca
1261 aacagggaag gcaaactccc gggcaaaagc ggacgaacat ggcgtgaagc ggatattaac
1321 tatacatcag gcttcagaaa ttcagaccgg attctttact caagcgactg gctgatttac
1381 aaaacaacgg accattatca gacctttaca aaaatcagat aatgtttaat gaccccgtgt
1441 cgagctctcg atctgatcga aggtcgtggt atccctcgta actctcgtgt tgatgcggga
1501 tccgaaatcg gtacaggctt ccccttcgac ccccattatg tggaagtcct gggcgagcgt
1561 atgcactacg tcgatgttgg accgcgggat ggcacgcctg tgctgttcct gcacggtaac
1621 ccgacctcgt cctacctgtg gcgcaacatc atcccgcatg tagcaccgag tcatcggtgc
1681 attgctccag acctgatcgg gatgggaaaa tcggacaaac cagacctcga ttatttcttc
1741 gacgaccacg tccgctacct cgatgccttc atcgaagcct tgggtttgga agaggtcgtc
1801 ctggtcatcc acgactgggg ctcagctctc ggattccact gggccaagcg caatccggaa
1861 cgggtcaaag gtattgcatg tatggaattc atccggccta tcccgacgtg ggacgaatgg
1921 ccagaattcg cccgtgagac cttccaggcc ttccggaccg ccgacgtcgg ccgagagttg
1981 atcatcgatc agaacgcttt catcgagggt gcgctcccga tgggggtcgt ccgtccgctt
2041 acggaggtcg agatggacca ctatcgcgag cccttcctca agcctgttga ccgagagcca
2101 ctgtggcgat tccccaacga gctgcccatc gccggtgagc ccgcgaacat cgtcgcgctc
2161 gtcgaggcat acatgaactg gctgcaccag tcacctgtcc cgaagttgtt gttctggggc
2221 acacccggcg tactgatccc cccggccgaa gccgcgagac ttgccgaaag cctccccaac
2281 tgcaagacag tggacatcgg cccgggattg ttcttgctcc aggaagacaa cccggacctt
2341 atcggcagtg agatcgcgcg ctggctcccg gcactctaat ctagagtcga cctgcaggca
2401 tgcaagctga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
2461 ctgagcaata actagcataa ccccttgggg cggccgcttc gagcagacat gataagatac
2521 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
2581 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
2641 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt ttttttaagc
2701 aagtaaaacc tctacaaatg tggtaaaatc gaattctaat ggatcctctt tgcgcttgcg
2761 ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc
2821 ggactggctt tctacgtgtt ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg
2881 cagcgtgagc ttcaaaagaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
2941 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
3001 ctgatcaaga gacaggatga cggtcgtttc gcatgcttga acaagatgga ttgcacgcag
3061 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
3121 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
3181 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
3241 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
3301 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
3361 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
3421 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgcact cggatggaag
3481 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
3541 tgttcgccag gctcaaggcg cgtatgccgg atggtgagga tctcgtcgtg actcatggcg
3601 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
3661 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
3721 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
3781 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
3841 gttcgaaatg accgaccaag cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata
3901 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
3961 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
4021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
4081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
4141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
4201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
4261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
4321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
4381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
4441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
4501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
4561 attacgcgca gaaaaaaagg atttcaagaa gatcctttga tcttttctac ggggtctgac
4621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
4681 ttcacctaga tccttttata gtccggaaat acaggaacgc acgctggatg gcccttcgct
4741 gggatggtga aaccatgaaa aatggcagct tcagtggatt aagtgggggt aatgtggcct
4801 gtaccctctg gttgcatagg tattcatacg gttaaaattt atcaggcgcg attgcggcag
4861 tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt
4921 tttagcggtg cgtacaatta agggattatg gtaaatccac ttactgtctg ccctcgtagc
4981 catcgagata aaccgcagta ctccggccac gatgcgtccg gcgtagagga tcgagatct
