pFC8A (HaloTag)
At a Glance
pFC8A, Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
ColE1 derived |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
CMV promoter |
| Accession Number | |
| edit table |
</protect>
Notes
- The HaloTag® is a "recombinant protein tag that affords flexibility between protein purification, expression and localization, protein interaction discovery, screening and further functional analysis. The technology is based on the formation of a covalent bond between a protein tag and synthetic ligands."
- pFC8A employs Sfg I, a rare endonuclease, for simple directional cloning. Various expression or peptide tag options to enable expression for protein analysis. All Flexi® Vectors possess the barnase gene, a lethal gene resulting in positive selection for insert ligation, as it is replaced by the DNA fragment of interest.
History
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Sources
Promega. Discontinued.
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggaa taagtaagga
1081 atccacatgg cacaggttat caacacgttt gacggggttg cggattatct tcagacatat
1141 cataagctac ctgataatta cattacaaaa tcagaagcac aagccctcgg ctgggtggca
1201 tcaaaaggga accttgcaga cgtcgctccg gggaaaagca tcggcggaga catcttctca
1261 aacagggaag gcaaactccc gggcaaaagc ggacgaacat ggcgtgaagc ggatattaac
1321 tatacatcag gcttcagaaa ttcagaccgg attctttact caagcgactg gctgatttac
1381 aaaacaacgg accattatca gacctttaca aaaatcagat aatgtttaat gaccccgtgt
1441 cgagctctcg atctgatcga aggtcgtggt atccctcgta actctcgtgt tgatgcggga
1501 tccgaaatcg gtacaggctt ccccttcgac ccccattatg tggaagtcct gggcgagcgt
1561 atgcactacg tcgatgttgg accgcgggat ggcacgcctg tgctgttcct gcacggtaac
1621 ccgacctcgt cctacctgtg gcgcaacatc atcccgcatg tagcaccgag tcatcggtgc
1681 attgctccag acctgatcgg gatgggaaaa tcggacaaac cagacctcga ttatttcttc
1741 gacgaccacg tccgctacct cgatgccttc atcgaagcct tgggtttgga agaggtcgtc
1801 ctggtcatcc acgactgggg ctcagctctc ggattccact gggccaagcg caatccggaa
1861 cgggtcaaag gtattgcatg tatggaattc atccggccta tcccgacgtg ggacgaatgg
1921 ccagaattcg cccgtgagac cttccaggcc ttccggaccg ccgacgtcgg ccgagagttg
1981 atcatcgatc agaacgcttt catcgagggt gcgctcccga tgggggtcgt ccgtccgctt
2041 acggaggtcg agatggacca ctatcgcgag cccttcctca agcctgttga ccgagagcca
2101 ctgtggcgat tccccaacga gctgcccatc gccggtgagc ccgcgaacat cgtcgcgctc
2161 gtcgaggcat acatgaactg gctgcaccag tcacctgtcc cgaagttgtt gttctggggc
2221 acacccggcg tactgatccc cccggccgaa gccgcgagac ttgccgaaag cctccccaac
2281 tgcaagacag tggacatcgg cccgggattg ttcttgctcc aggaagacaa cccggacctt
2341 atcggcagtg agatcgcgcg ctggctcccg gcactctaat ctagagtcga cctgcaggca
2401 tgcaagctga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
2461 ctgagcaata actagcataa ccccttgggg cggccgcttc gagcagacat gataagatac
2521 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
2581 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
2641 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt ttttttaagc
2701 aagtaaaacc tctacaaatg tggtaaaatc gaattctaat ggatcctctt tgcgcttgcg
2761 ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc
2821 ggactggctt tctacgtaat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
2881 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
2941 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
3001 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
3061 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
3121 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
3181 atgatgagca ctttcaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
3241 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
3301 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
3361 accatgagtg ataacactgc ggccaactta cttctgacaa ctatcggagg accgaaggag
3421 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
3481 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
3541 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
3601 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
3661 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
3721 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
3781 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
3841 tggtaattcg aaatgaccga ccaagcgacg cccaaccggt atcagctcac tcaaaggcgg
3901 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
3961 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
4021 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
4081 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
4141 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
4201 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
4261 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
4321 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
4381 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
4441 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
4501 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
4561 agcagattac gcgcagaaaa aaaggatttc aagaagatcc tttgatcttt tctacggggt
4621 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
4681 ggatcttcac ctagatcctt ttatagtccg gaaatacagg aacgcacgct ggatggccct
4741 tcgctgggat ggtgaaacca tgaaaaatgg cagcttcagt ggattaagtg ggggtaatgt
4801 ggcctgtacc ctctggttgc ataggtattc atacggttaa aatttatcag gcgcgattgc
4861 ggcagttttt cgggtggttt gttgccattt ttacctgtct gctgccgtga tcgcgctgaa
4921 cgcgttttag cggtgcgtac aattaaggga ttatggtaaa tccacttact gtctgccctc
4981 gtagccatcg agataaaccg cagtactccg gccacgatgc gtccggcgta gaggatcgag
5041 atct
