pFC7K (HQ)
At a Glance
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pFC7K (HQ) Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
ColE1 |
| Copy # | |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number | |
| edit table |
</protect>
Notes
This vector is designed for for expressing C-terminal, HisGln (HQ) metal-binding peptide fusion proteins in bacteria and in vitro protein expression systems via the T7 RNA promoter. There is a HQ tag that attaches to the C-terminus of a protein and can be used to purify the expressed protein using the MagneHis, MagZ, HisLink 96 or HisLink Protein Purification Systems. This vector, lacks the Pme I site, but contains the Sgf I site. In addition, it also uses the blunt-end site EcoICR I to create a readthrough sequence when joined to a protein-coding region connected by Sgf I and Pme I. Unlike pFC7A (HQ), this vector is kanamycin resistant. It also purifies protein through a metal affinity resin.
History
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Sources
References
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Sequence
1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGA AATTTTAACT 51 TTACTAAGGA GCGATCGCCA TGGCACAGGT TATCAACACG TTTGACGGGG 101 TTGCGGATTA TCTTCAGACA TATCATAAGC TACCTGATAA TTACATTACA 151 AAATCAGAAG CACAAGCCCT CGGCTGGGTG GCATCAAAAG GGAACCTTGC 201 AGACGTCGCT CCGGGGAAAA GCATCGGCGG AGACATCTTC TCAAACAGGG 251 AAGGCAAACT CCCGGGCAAA AGCGGACGAA CATGGCGTGA AGCGGATATT 301 AACTATACAT CAGGCTTCAG AAATTCAGAC CGGATTCTTT ACTCAAGCGA 351 CTGGCTGATT TACAAAACAA CGGACCATTA TCAGACCTTT ACAAAAATCA 401 GATAATGTTT AATGACCCCG TGTCGAGCTC ATCAACACCA ACATCAATAA 451 TCTAGAGTCG ACCTGCAGGC ATGCAAGCTG ATCCGGCTGC TAACAAAGCC 501 CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA 551 ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG 601 GAACTATATC CGGTTCGCTT GCTGTCCATA AAACCGCCCA GTCTAGCTAT 651 CGCCATGTAA GCCCACTGCA AGCTACCTGC TTTCTCTTTG CGCTTGCGTT 701 TTCCCTTGTC CAGATAGCCC AGTAGCTGAC ATTCATCCGG GGTCAGCACC 751 GTTTCTGCGG ACTGGCTTTC TACGTGTTCC GCTTCCTTTA GCAGCCCTTG 801 CGCCCTGAGT GCTTGCGGCA GCGTGAGCTT CAAAAGAATT GCCAGCTGGG 851 GCGCCCTCTG GTAAGGTTGG GAAGCCCTGC AAAGTAAACT GGATGGCTTT 901 CTTGCCGCCA AGGATCTGAT GGCGCAGGGG ATCAAGATCT GATCAAGAGA 951 CAGGATGACG GTCGTTTCGC ATGCTTGAAC AAGATGGATT GCACGCAGGT 1001 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA 1051 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC 1101 GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 1151 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 1201 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT 1251 TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 1301 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA 1351 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG 1401 CACGCACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 1451 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG 1501 TATGCCGGAT GGTGAGGATC TCGTCGTGAC TCATGGCGAT GCCTGCTTGC 1551 CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 1601 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA 1651 TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT 1701 ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 1751 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG 1801 ACGCCCAACC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 1851 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 1901 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 1951 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 2001 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 2051 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 2101 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 2151 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 2201 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 2251 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 2301 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 2351 GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC 2401 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 2451 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 2501 TACGCGCAGA AAAAAAGGAT TTCAAGAAGA TCCTTTGATC TTTTCTACGG 2551 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 2601 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTATAGT CCGGAAATAC 2651 AGGAACGCAC GCTGGATGGC CCTTCGCTGG GATGGTGAAA CCATGAAAAA 2701 TGGCAGCTTC AGTGGATTAA GTGGGGGTAA TGTGGCCTGT ACCCTCTGGT 2751 TGCATAGGTA TTCATACGGT TAAAATTTAT CAGGCGCGAT TGCGGCAGTT 2801 TTTCGGGTGG TTTGTTGCCA TTTTTACCTG TCTGCTGCCG TGATCGCGCT 2851 GAACGCGTTT TAGCGGTGCG TACAATTAAG GGATTATGGT AAATCCACTT 2901 ACTGTCTGCC CTCGTAGCCA TCGAGATAAA CCGCAGTACT CCGGCCACGA 2951 TGCGTCCGGC GTAGAGGATC GAGATCTTTT CAGCCTGATA CAGATTAAAT 3001 CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG CGGCAGTAGC 3051 GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG 3101 CGCCGATGGT AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG 3151 CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT 3201 CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG 3251 CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG GGCAGGACGC 3301 CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA 3351 TGGCCTTTTT GCGTTTCTAC AAACTCTTTT GTTTATTTTT CTAAATACAT 3401 TCAAATATGT ATCCGCTCAT
