pFC7A (HQ)
At a Glance
.jpg)
pFC7A (HQ), Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
ColE1 |
| Copy # | |
| Markers |
Ampicillin, Kanamycin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number | |
| edit table |
</protect>
Notes
This vector is designed for for expressing C-terminal, HisGln (HQ) metal-binding peptide fusion proteins in bacteria and in vitro protein expression systems via the T7 RNA promoter. There is a HQ tag that attaches to the C-terminus of a protein and can be used to purify the expressed protein using the MagneHis, MagZ, HisLink 96 or HisLink Protein Purification Systems. This vector, lacks the Pme I site, but contains the Sgf I site. In addition, it also uses the blunt-end site EcoICR I to create a readthrough sequence when joined to a protein-coding region connected by Sgf I and Pme I. Unlike pFC7K (HQ), this vector is ampicilin resistant. It also purifies protein through a metal affinity resin.
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGA AATTTTAACT 51 TTACTAAGGA GCGATCGCCA TGGCACAGGT TATCAACACG TTTGACGGGG 101 TTGCGGATTA TCTTCAGACA TATCATAAGC TACCTGATAA TTACATTACA 151 AAATCAGAAG CACAAGCCCT CGGCTGGGTG GCATCAAAAG GGAACCTTGC 201 AGACGTCGCT CCGGGGAAAA GCATCGGCGG AGACATCTTC TCAAACAGGG 251 AAGGCAAACT CCCGGGCAAA AGCGGACGAA CATGGCGTGA AGCGGATATT 301 AACTATACAT CAGGCTTCAG AAATTCAGAC CGGATTCTTT ACTCAAGCGA 351 CTGGCTGATT TACAAAACAA CGGACCATTA TCAGACCTTT ACAAAAATCA 401 GATAATGTTT AATGACCCCG TGTCGAGCTC ATCAACACCA ACATCAATAA 451 TCTAGAGTCG ACCTGCAGGC ATGCAAGCTG ATCCGGCTGC TAACAAAGCC 501 CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA 551 ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG 601 GAACTATATC CGGTTCGCTT GCTGTCCATA AAACCGCCCA GTCTAGCTAT 651 CGCCATGTAA GCCCACTGCA AGCTACCTGC TTTCTCTTTG CGCTTGCGTT 701 TTCCCTTGTC CAGATAGCCC AGTAGCTGAC ATTCATCCGG GGTCAGCACC 751 GTTTCTGCGG ACTGGCTTTC TACGTAATGG TTTCTTAGAC GTCAGGTGGC 801 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT 851 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC 901 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC 951 CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA 1001 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 1051 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC 1101 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTCAAAGTTC TGCTATGTGG 1151 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA 1201 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG 1251 CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC 1301 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACT ATCGGAGGAC 1351 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC 1401 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG 1451 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA 1501 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG 1551 GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG 1601 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 1651 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC 1701 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC 1751 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAATTCGAA ATGACCGACC 1801 AAGCGACGCC CAACCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 1851 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG 1901 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 1951 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 2001 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 2051 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 2101 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 2151 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 2201 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 2251 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 2301 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 2351 AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG 2401 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 2451 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 2501 CAGATTACGC GCAGAAAAAA AGGATTTCAA GAAGATCCTT TGATCTTTTC 2551 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 2601 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT ATAGTCCGGA 2651 AATACAGGAA CGCACGCTGG ATGGCCCTTC GCTGGGATGG TGAAACCATG 2701 AAAAATGGCA GCTTCAGTGG ATTAAGTGGG GGTAATGTGG CCTGTACCCT 2751 CTGGTTGCAT AGGTATTCAT ACGGTTAAAA TTTATCAGGC GCGATTGCGG 2801 CAGTTTTTCG GGTGGTTTGT TGCCATTTTT ACCTGTCTGC TGCCGTGATC 2851 GCGCTGAACG CGTTTTAGCG GTGCGTACAA TTAAGGGATT ATGGTAAATC 2901 CACTTACTGT CTGCCCTCGT AGCCATCGAG ATAAACCGCA GTACTCCGGC 2951 CACGATGCGT CCGGCGTAGA GGATCGAGAT CTTTTCAGCC TGATACAGAT 3001 TAAATCAGAA CGCAGAAGCG GTCTGATAAA ACAGAATTTG CCTGGCGGCA 3051 GTAGCGCGGT GGTCCCACCT GACCCCATGC CGAACTCAGA AGTGAAACGC 3101 CGTAGCGCCG ATGGTAGTGT GGGGTCTCCC CATGCGAGAG TAGGGAACTG 3151 CCAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT 3201 TTTATCTGTT GTTTGTCGGT GAACGCTCTC CTGAGTAGGA CAAATCCGCC 3251 GGGAGCGGAT TTGAACGTTG CGAAGCAACG GCCCGGAGGG TGGCGGGCAG 3301 GACGCCCGCC ATAAACTGCC AGGCATCAAA TTAAGCAGAA GGCCATCCTG 3351 ACGGATGGCC TTTTTGCGTT TCTACAAACT CTTTTGTTTA TTTTTCTAAA 3401 TACATTCAAA TATGTATCCG CTCAT
