pF9A CMV hRluc-neo
At a Glance
![](/colipedia/images/thumb/b/b9/PF9A_CMV_hRluc-neo.jpg/450px-PF9A_CMV_hRluc-neo.jpg)
pF9A CMV hRluc-neo, Image made with PlasMapper
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Type |
Cloning |
---|---|
Origin of Replication |
ColE1 derived |
Copy # | |
Markers |
Ampicillin |
Link to Sequence | |
Features |
CMV promoter |
Accession Number | |
edit table |
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Notes
pF9A CMV hRluc-neo employs Sfg I & Pme I, a rare endonuclease, for simple directional cloning. Various expression or peptide tag options to enable expression for protein analysis. All Flexi® Vectors possess the barnase gene, a lethal gene resulting in positive selection for insert ligation, as it is replaced by the DNA fragment of interest.
History
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Sources
References
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Sequence
1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat 781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc 841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa 901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac 961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta 1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggaa taagtaagga 1081 atccacatgg cacaggttat caacacgttt gacggggttg cggattatct tcagacatat 1141 cataagctac ctgataatta cattacaaaa tcagaagcac aagccctcgg ctgggtggca 1201 tcaaaaggga accttgcaga cgtcgctccg gggaaaagca tcggcggaga catcttctca 1261 aacagggaag gcaaactccc gggcaaaagc ggacgaacat ggcgtgaagc ggatattaac 1321 tatacatcag gcttcagaaa ttcagaccgg attctttact caagcgactg gctgatttac 1381 aaaacaacgg accattatca gacctttaca aaaatcagat aatgtttaaa cgaattcggg 1441 ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagctgat ccggctgcta 1501 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac 1561 cccttggggc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc 1621 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta 1681 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg 1741 tttcaggttc agggggagat gtgggaggtt tttttaagca agtaaaacct ctacaaatgt 1801 ggtaaaatcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc 1861 cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca ccatggcctg 1921 aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt 1981 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 2041 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 2101 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 2161 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 2221 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 2281 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tgattcttct gacacaacag 2341 tctcgaacca aaggctggag ccaccatggc ttccaaggtg tacgaccccg agcaacgcaa 2401 acgcatgatc actgggcctc agtggtgggc tcgctgcaag caaatgaacg tgctggactc 2461 cttcatcaac tactatgatt ccgagaagca cgccgagaac gccgtgattt ttctgcatgg 2521 taacgctgcc tccagctacc tgtggaggca cgtcgtgcct cacatcgagc ccgtggctag 2581 atgcatcatc cctgatctga tcggaatggg taagtccggc aagagcggga atggctcata 2641 tcgcctcctg gatcactaca agtacctcac cgcttggttc gagctgctga accttccaaa 2701 gaaaatcatc tttgtgggcc acgactgggg ggcttgtctg gcctttcact actcctacga 2761 gcaccaagac aagatcaagg ccatcgtcca tgctgagagt gtcgtggacg tgatcgagtc 2821 ctgggacgag tggcctgaca tcgaggagga tatcgccctg atcaagagcg aagagggcga 2881 gaaaatggtg cttgagaata acttcttcgt cgagaccatg ctcccaagca agatcatgcg 2941 gaaactggag cctgaggagt tcgctgccta cctggagcca ttcaaggaga agggcgaggt 3001 tagacggcct accctctcct ggcctcgcga gatccctctc gttaagggag gcaagcccga 3061 cgtcgtccag attgtccgca actacaacgc ctaccttcgg gccagcgacg atctgcctaa 3121 gatgttcatc gagtccgacc ctgggttctt ttccaacgct attgtcgagg gagctaagaa 3181 gttccctaac accgagttcg tgaaggtgaa gggcctccac ttcagccagg aggacgctcc 3241 agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc gtgctgaaga acgagcagac 3301 cggtggtggg agcggaggtg gcggatcagg tggcggaggc tccggaggga ttgaacaaga 3361 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 3421 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 3481 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 3541 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 3601 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 3661 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 3721 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 3781 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 3841 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 3901 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 3961 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 4021 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 4081 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 4141 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgatgg 4201 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat 4261 agcgataagg atcctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac 4321 attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtaatgg tttcttagac 4381 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 4441 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 4501 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 4561 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 4621 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 4681 gagttttcgc cccgaagaac gttttccaat gatgagcact ttcaaagttc tgctatgtgg 4741 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 4801 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 4861 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 4921 tctgacaact atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 4981 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 5041 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 5101 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 5161 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 5221 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 5281 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 5341 tgagataggt gcctcactga ttaagcattg gtaattcgaa atgaccgacc aagcgacgcc 5401 caaccggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 5461 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 5521 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 5581 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 5641 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 5701 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 5761 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 5821 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 5881 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 5941 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 6001 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 6061 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatttcaa 6121 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 6181 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt atagtccgga 6241 aatacaggaa cgcacgctgg atggcccttc gctgggatgg tgaaaccatg aaaaatggca 6301 gcttcagtgg attaagtggg ggtaatgtgg cctgtaccct ctggttgcat aggtattcat 6361 acggttaaaa tttatcaggc gcgattgcgg cagtttttcg ggtggtttgt tgccattttt 6421 acctgtctgc tgccgtgatc gcgctgaacg cgttttagcg gtgcgtacaa ttaagggatt 6481 atggtaaatc cacttactgt ctgccctcgt agccatcgag ataaaccgca gtactccggc 6541 cacgatgcgt ccggcgtaga ggatcgagat ct