pF9A CMV hRluc-neo
At a Glance
pF9A CMV hRluc-neo, Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
ColE1 derived |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
CMV promoter |
| Accession Number | |
| edit table |
</protect>
Notes
pF9A CMV hRluc-neo employs Sfg I & Pme I, a rare endonuclease, for simple directional cloning. Various expression or peptide tag options to enable expression for protein analysis. All Flexi® Vectors possess the barnase gene, a lethal gene resulting in positive selection for insert ligation, as it is replaced by the DNA fragment of interest.
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Sources
References
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Sequence
1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggaa taagtaagga
1081 atccacatgg cacaggttat caacacgttt gacggggttg cggattatct tcagacatat
1141 cataagctac ctgataatta cattacaaaa tcagaagcac aagccctcgg ctgggtggca
1201 tcaaaaggga accttgcaga cgtcgctccg gggaaaagca tcggcggaga catcttctca
1261 aacagggaag gcaaactccc gggcaaaagc ggacgaacat ggcgtgaagc ggatattaac
1321 tatacatcag gcttcagaaa ttcagaccgg attctttact caagcgactg gctgatttac
1381 aaaacaacgg accattatca gacctttaca aaaatcagat aatgtttaaa cgaattcggg
1441 ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagctgat ccggctgcta
1501 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
1561 cccttggggc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
1621 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
1681 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
1741 tttcaggttc agggggagat gtgggaggtt tttttaagca agtaaaacct ctacaaatgt
1801 ggtaaaatcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
1861 cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca ccatggcctg
1921 aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt
1981 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
2041 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
2101 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
2161 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
2221 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
2281 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tgattcttct gacacaacag
2341 tctcgaacca aaggctggag ccaccatggc ttccaaggtg tacgaccccg agcaacgcaa
2401 acgcatgatc actgggcctc agtggtgggc tcgctgcaag caaatgaacg tgctggactc
2461 cttcatcaac tactatgatt ccgagaagca cgccgagaac gccgtgattt ttctgcatgg
2521 taacgctgcc tccagctacc tgtggaggca cgtcgtgcct cacatcgagc ccgtggctag
2581 atgcatcatc cctgatctga tcggaatggg taagtccggc aagagcggga atggctcata
2641 tcgcctcctg gatcactaca agtacctcac cgcttggttc gagctgctga accttccaaa
2701 gaaaatcatc tttgtgggcc acgactgggg ggcttgtctg gcctttcact actcctacga
2761 gcaccaagac aagatcaagg ccatcgtcca tgctgagagt gtcgtggacg tgatcgagtc
2821 ctgggacgag tggcctgaca tcgaggagga tatcgccctg atcaagagcg aagagggcga
2881 gaaaatggtg cttgagaata acttcttcgt cgagaccatg ctcccaagca agatcatgcg
2941 gaaactggag cctgaggagt tcgctgccta cctggagcca ttcaaggaga agggcgaggt
3001 tagacggcct accctctcct ggcctcgcga gatccctctc gttaagggag gcaagcccga
3061 cgtcgtccag attgtccgca actacaacgc ctaccttcgg gccagcgacg atctgcctaa
3121 gatgttcatc gagtccgacc ctgggttctt ttccaacgct attgtcgagg gagctaagaa
3181 gttccctaac accgagttcg tgaaggtgaa gggcctccac ttcagccagg aggacgctcc
3241 agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc gtgctgaaga acgagcagac
3301 cggtggtggg agcggaggtg gcggatcagg tggcggaggc tccggaggga ttgaacaaga
3361 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
3421 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
3481 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
3541 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
3601 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
3661 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
3721 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
3781 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
3841 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
3901 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
3961 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
4021 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
4081 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
4141 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgatgg
4201 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
4261 agcgataagg atcctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac
4321 attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtaatgg tttcttagac
4381 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
4441 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
4501 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
4561 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
4621 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
4681 gagttttcgc cccgaagaac gttttccaat gatgagcact ttcaaagttc tgctatgtgg
4741 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
4801 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
4861 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
4921 tctgacaact atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
4981 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
5041 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
5101 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
5161 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
5221 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
5281 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
5341 tgagataggt gcctcactga ttaagcattg gtaattcgaa atgaccgacc aagcgacgcc
5401 caaccggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
5461 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
5521 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
5581 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
5641 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
5701 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
5761 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
5821 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
5881 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
5941 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
6001 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
6061 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatttcaa
6121 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
6181 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt atagtccgga
6241 aatacaggaa cgcacgctgg atggcccttc gctgggatgg tgaaaccatg aaaaatggca
6301 gcttcagtgg attaagtggg ggtaatgtgg cctgtaccct ctggttgcat aggtattcat
6361 acggttaaaa tttatcaggc gcgattgcgg cagtttttcg ggtggtttgt tgccattttt
6421 acctgtctgc tgccgtgatc gcgctgaacg cgttttagcg gtgcgtacaa ttaagggatt
6481 atggtaaatc cacttactgt ctgccctcgt agccatcgag ataaaccgca gtactccggc
6541 cacgatgcgt ccggcgtaga ggatcgagat ct
