pF3K WG (BYDV)
At a Glance
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pF3K WG (BYDV), Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication | |
| Copy # | |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
SP6 promoter |
| Accession Number | |
| edit table |
</protect>
Notes
This vector is one of two vectors designed specifically for in vitro expression of native protein in wheat germ extracts. The Wheat Germ Extrast Plus is a single tube protein, and is proved highly efficient in it's function. There are tRNA, ribosomes, amino acids initiation factos, elongation factors and termination factors that play a role in protein synthesis. In addition, potassium acetate is added at certain concentrations to enhance the translation of a wide range of RNA's. The addition of an mRNA template and incubation of the reaction initiates the protein synthesis reaction. This vector contains sequences from barley yellow dwarf virus (BYDV) and RNA plant virus located both downstream and upstream of the protein coding region of interest.
History
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Sources
References
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Sequence
1 GGATCTCGAT CCCGCGAAAT TAATTTAGGT GACACTATAG AAGGGTAATA 51 CGACTCACTA TAGCAGTGAA GATTGACCAT CTCACAAAAG CTGTTACGTG 101 CTTGTAACAC ACTACACACT CGTTTTGTAT TCGAGAAGTA GTTGCAACAA 151 CGGTCCCCTT ATTGCCTGAC AAGCTGAGGG CCACCCTTCT ATCCCCACCG 201 CGCGATCGCC ATGGAATAAG TAAGGAATCC ACATGGCACA GGTTATCAAC 251 ACGTTTGACG GGGTTGCGGA TTATCTTCAG ACATATCATA AGCTACCTGA 301 TAATTACATT ACAAAATCAG AAGCACAAGC CCTCGGCTGG GTGGCATCAA 351 AAGGGAACCT TGCAGACGTC GCTCCGGGGA AAAGCATCGG CGGAGACATC 401 TTCTCAAACA GGGAAGGCAA ACTCCAGGGC AAAAGCGGAC GAACATGGCG 451 TGAAGCGGAT ATTAACTATA CATCAGGCTT CAGAAATTCA GACCGGATTC 501 TTTACTCAAG CGACTGGCTG ATTTACAAAA CAACGGACCA TTATCAGACC 551 TTTACAAAAA TCAGATAATG TTTAAACGAA TTCGAGCTCG GTACCAGTGA 601 AGACAACACC ACTAGCACAA ATCGGATCCT GGGAAACAGG CAGAACTTCG 651 GTTCATAAGC TCGGGTAGGC TGTCAACCTA CCGCCGTATC GTATTGTGTT 701 TGTCTAGATA CGTATCGCGA GTCGACCTGC AGGCATGCAA GCTGATCCGG 751 CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG 801 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT 851 TTTGCTGAAA GGAGGAACTA TATCCGGTTC GCTTGCTGTC CATAAAACCG 901 CCCAGTCTAG CTATCGCCAT GTAAGCCCAC TGCAAGCTAC CTGCTTTCTC 951 TTTGCGCTTG CGTTTTCCCT TGTCCAGATA GCCCAGTAGC TGACATTCAT 1001 CCGGGGTCAG CACCGTTTCT GCGGACTGGC TTTCTACGTG TTCCGCTTCC 1051 TTTAGCAGCC CTTGCGCCCT GAGTGCTTGC GGCAGCGTGA GCTTCAAAAG 1101 AATTGCCAGC TGGGGCGCCC TCTGGTAAGG TTGGGAAGCC CTGCAAAGTA 1151 AACTGGATGG CTTTCTTGCC GCCAAGGATC TGATGGCGCA GGGGATCAAG 1201 ATCTGATCAA GAGACAGGAT GACGGTCGTT TCGCATGCTT GAACAAGATG 1251 GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 1301 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT 1351 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG 1401 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 1451 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG 1501 GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC 1551 ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 1601 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA 1651 TCGCATCGAG CGAGCACGCA CTCGGATGGA AGCCGGTCTT GTCGATCAGG 1701 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 1751 AGGCTCAAGG CGCGTATGCC GGATGGTGAG GATCTCGTCG TGACTCATGG 1801 CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT 1851 TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG 1901 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG 1951 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT 2001 TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 2051 TGACCGACCA AGCGACGCCC AACCGGTATC AGCTCACTCA AAGGCGGTAA 2101 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA 2151 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 2201 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 2251 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 2301 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 2351 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 2401 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC 2451 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 2501 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 2551 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 2601 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT 2651 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 2701 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT 2751 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATTTCAAG AAGATCCTTT 2801 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 2851 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 2901 TAGTCCGGAA ATACAGGAAC GCACGCTGGA TGGCCCTTCG CTGGGATGGT 2951 GAAACCATGA AAAATGGCAG CTTCAGTGGA TTAAGTGGGG GTAATGTGGC 3001 CTGTACCCTC TGGTTGCATA GGTATTCATA CGGTTAAAAT TTATCAGGCG 3051 CGATTGCGGC AGTTTTTCGG GTGGTTTGTT GCCATTTTTA CCTGTCTGCT 3101 GCCGTGATCG CGCTGAACGC GTTTTAGCGG TGCGTACAAT TAAGGGATTA 3151 TGGTAAATCC ACTTACTGTC TGCCCTCGTA GCCATCGAGA TAAACCGCAG 3201 TACTCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TTTTCAGCCT 3251 GATACAGATT AAATCAGAAC GCAGAAGCGG TCTGATAAAA CAGAATTTGC 3301 CTGGCGGCAG TAGCGCGGTG GTCCCACCTG ACCCCATGCC GAACTCAGAA 3351 GTGAAACGCC GTAGCGCCGA TGGTAGTGTG GGGTCTCCCC ATGCGAGAGT 3401 AGGGAACTGC CAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG 3451 GCCTTTCGTT TTATCTGTTG TTTGTCGGTG AACGCTCTCC TGAGTAGGAC 3501 AAATCCGCCG GGAGCGGATT TGAACGTTGC GAAGCAACGG CCCGGAGGGT 3551 GGCGGGCAGG ACGCCCGCCA TAAACTGCCA GGCATCAAAT TAAGCAGAAG 3601 GCCATCCTGA CGGATGGCCT TTTTGCGTTT CTACAAACTC TTTTGTTTAT 3651 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCAT
