pF3A WG (BYDV)
At a Glance
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<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication | |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
SP6 promoter |
| Accession Number | |
| edit table |
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Notes
The pF3A WG(BYDV) vector is one of two vectors inthe Wheat Germ Extract Plus. The Wheat Germ Extrast Plus is a highly efficient, single tube protein expression. In the extract exists all cellular substances that are required for protein synthesis including tRNA, ribosomes, amino acids, initiation factors, elongation factors, and termination factors.To enhance the translation of a wide range of RNAs potassium acetate is added at a certain concentration. The initation of protein synthesis is started by the addition of an mRNA template and the reaction is then incubated for 2 hours.
The vectors pF3A WG (BYDV) and pF3K WG (BYDV) are both designed for in vitro expression in wheat germ extracts. Contained in vectors are sequences from barley yellow dwarf virus (BYDV) and RNA plant virus. They are located downstream and upstream of the protein coding region of interest.
History
This vector is also part of the Flexi Cloning System. It is including in this group of vectors.
Sources
References
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Sequence
1 GGATCTCGAT CCCGCGAAAT TAATTTAGGT GACACTATAG AAGGGTAATA 51 CGACTCACTA TAGCAGTGAA GATTGACCAT CTCACAAAAG CTGTTACGTG 101 CTTGTAACAC ACTACACACT CGTTTTGTAT TCGAGAAGTA GTTGCAACAA 151 CGGTCCCCTT ATTGCCTGAC AAGCTGAGGG CCACCCTTCT ATCCCCACCG 201 CGCGATCGCC ATGGAATAAG TAAGGAATCC ACATGGCACA GGTTATCAAC 251 ACGTTTGACG GGGTTGCGGA TTATCTTCAG ACATATCATA AGCTACCTGA 301 TAATTACATT ACAAAATCAG AAGCACAAGC CCTCGGCTGG GTGGCATCAA 351 AAGGGAACCT TGCAGACGTC GCTCCGGGGA AAAGCATCGG CGGAGACATC 401 TTCTCAAACA GGGAAGGCAA ACTCCAGGGC AAAAGCGGAC GAACATGGCG 451 TGAAGCGGAT ATTAACTATA CATCAGGCTT CAGAAATTCA GACCGGATTC 501 TTTACTCAAG CGACTGGCTG ATTTACAAAA CAACGGACCA TTATCAGACC 551 TTTACAAAAA TCAGATAATG TTTAAACGAA TTCGAGCTCG GTACCAGTGA 601 AGACAACACC ACTAGCACAA ATCGGATCCT GGGAAACAGG CAGAACTTCG 651 GTTCATAAGC TCGGGTAGGC TGTCAACCTA CCGCCGTATC GTATTGTGTT 701 TGTCTAGATA CGTATCGCGA GTCGACCTGC AGGCATGCAA GCTGATCCGG 751 CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG 801 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT 851 TTTGCTGAAA GGAGGAACTA TATCCGGTTC GCTTGCTGTC CATAAAACCG 901 CCCAGTCTAG CTATCGCCAT GTAAGCCCAC TGCAAGCTAC CTGCTTTCTC 951 TTTGCGCTTG CGTTTTCCCT TGTCCAGATA GCCCAGTAGC TGACATTCAT 1001 CCGGGGTCAG CACCGTTTCT GCGGACTGGC TTTCTACGTA ATGGTTTCTT 1051 AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 1101 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 1151 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC 1201 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT 1251 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 1301 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC 1351 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTCAAA 1401 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA 1451 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC 1501 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC 1551 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC 1601 AACTATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG 1651 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 1701 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 1751 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT 1801 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG 1851 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG 1901 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC 1951 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 2001 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAATT 2051 CGAAATGACC GACCAAGCGA CGCCCAACCG GTATCAGCTC ACTCAAAGGC 2101 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 2151 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 2201 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 2251 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 2301 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 2351 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 2401 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 2451 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 2501 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 2551 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 2601 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA 2651 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 2701 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT 2751 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATT TCAAGAAGAT 2801 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 2851 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 2901 TTTTATAGTC CGGAAATACA GGAACGCACG CTGGATGGCC CTTCGCTGGG 2951 ATGGTGAAAC CATGAAAAAT GGCAGCTTCA GTGGATTAAG TGGGGGTAAT 3001 GTGGCCTGTA CCCTCTGGTT GCATAGGTAT TCATACGGTT AAAATTTATC 3051 AGGCGCGATT GCGGCAGTTT TTCGGGTGGT TTGTTGCCAT TTTTACCTGT 3101 CTGCTGCCGT GATCGCGCTG AACGCGTTTT AGCGGTGCGT ACAATTAAGG 3151 GATTATGGTA AATCCACTTA CTGTCTGCCC TCGTAGCCAT CGAGATAAAC 3201 CGCAGTACTC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTTTTC 3251 AGCCTGATAC AGATTAAATC AGAACGCAGA AGCGGTCTGA TAAAACAGAA 3301 TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC ACCTGACCCC ATGCCGAACT 3351 CAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC TCCCCATGCG 3401 AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG 3451 ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT 3501 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG 3551 AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAATTAAGC 3601 AGAAGGCCAT CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTTTG 3651 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCAT
