pF1K T7
At a Glance
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
ColE1 |
| Copy # | |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 promoter |
| Accession Number | |
| edit table |
</protect>
Notes
The pF1K T7 vector is part of a group of plasmids that are all grouped under the Flexi Vector System. This system is a powerful directional cloning methof for protein-coding sequences. The basis for the method is the two restriction enzymes SgfI(d) and PmeI(c). These are two rare-cutting restriction enzy,es that provide a rapid, efficient way to transfer protein-coding regions back and forth between the Flexi vectors. Using these restriction enzymes elimates the need to resequence. By replacing the DNA sequence of interest by means of the lethal barnase gene contained in each vector, it acts as a positive selection for he successful ligation of the insert.
Use of the Flexi Vectors eliminates the need for appending multiple amino acids to the N- or C-termini of the protein of interest like so many other vectors require. This vector system also does not require the use of an archival entry vector.
History
The Flexi vector system differs from plasmid to plasmid in the resistant genes and other very small factors.
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG
51 CGGATAACAA TTCCCCACTA GTAATAATTT TCTTTAACTT TAGTAAGGAG
101 CGATCGCCAT GGCACAGGTT ATCAACACGT TTGACGGGGT TGCGGATTAT
151 CTTCAGACAT ATCATAAGCT ACCTGATAAT TACATTACAA AATCAGAAGC
201 ACAAGCCCTC GGCTGGGTGG CATCAAAAGG GAACCTTGCA GACGTCGCTC
251 CGGGGAAAAG CATCGGCGGA GACATCTTCT CAAACAGGGA AGGCAAACTC
301 CAGGGCAAAA GCGGACGAAC ATGGCGTGAA GCGGATATTA ACTATACATC
351 AGGCTTCAGA AATTCAGACC GGATTCTTTA CTCAAGCGAC TGGCTGATTT
401 ACAAAACAAC GGACCATTAT CAGACCTTTA CAAAAATCAG ATAATGTTTA
451 AACGAATTCG AGCTCGGTAC CCGGGGATCC TCTAGAGTCG ACCTGCAGGC
501 ATGCAAGCTG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC
551 TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC
601 GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGTTCGCTT
651 GCTGTCCATA AAACCGCCCA GTCTAGCTAT CGCCATGTAA GCCCACTGCA
701 AGCTACCTGC TTTCTCTTTG CGCTTGCGTT TTCCCTTGTC CAGATAGCCC
751 AGTAGCTGAC ATTCATCCGG GGTCAGCACC GTTTCTGCGG ACTGGCTTTC
801 TACGTGTTCC GCTTCCTTTA GCAGCCCTTG CGCCCTGAGT GCTTGCGGCA
851 GCGTGAGCTT CAAAAGAATT GCCAGCTGGG GCGCCCTCTG GTAAGGTTGG
901 GAAGCCCTGC AAAGTAAACT GGATGGCTTT CTTGCCGCCA AGGATCTGAT
951 GGCGCAGGGG ATCAAGATCT GATCAAGAGA CAGGATGACG GTCGTTTCGC
1001 ATGCTTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
1051 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG
1101 CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
1151 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT
1201 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG
1251 TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG
1301 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
1351 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG
1401 ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGCACTCG GATGGAAGCC
1451 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC
1501 AGCCGAACTG TTCGCCAGGC TCAAGGCGCG TATGCCGGAT GGTGAGGATC
1551 TCGTCGTGAC TCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT
1601 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
1651 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG
1701 GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT
1751 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG
1801 ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC GGTATCAGCT
1851 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
1901 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG
1951 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
2001 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
2051 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
2101 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
2151 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
2201 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
2251 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
2301 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
2351 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
2401 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC
2451 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
2501 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
2551 TTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
2601 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
2651 CACCTAGATC CTTTTATAGT CCGGAAATAC AGGAACGCAC GCTGGATGGC
2701 CCTTCGCTGG GATGGTGAAA CCATGAAAAA TGGCAGCTTC AGTGGATTAA
2751 GTGGGGGTAA TGTGGCCTGT ACCCTCTGGT TGCATAGGTA TTCATACGGT
2801 TAAAATTTAT CAGGCGCGAT TGCGGCAGTT TTTCGGGTGG TTTGTTGCCA
2851 TTTTTACCTG TCTGCTGCCG TGATCGCGCT GAACGCGTTT TAGCGGTGCG
2901 TACAATTAAG GGATTATGGT AAATCCACTT ACTGTCTGCC CTCGTAGCCA
2951 TCGAGATAAA CCGCAGTACT CCGGCCACGA TGCGTCCGGC GTAGAGGATC
3001 GAGATCTTTT CAGCCTGATA CAGATTAAAT CAGAACGCAG AAGCGGTCTG
3051 ATAAAACAGA ATTTGCCTGG CGGCAGTAGC GCGGTGGTCC CACCTGACCC
3101 CATGCCGAAC TCAGAAGTGA AACGCCGTAG CGCCGATGGT AGTGTGGGGT
3151 CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATCAAATAA AACGAAAGGC
3201 TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG
3251 CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG
3301 CAACGGCCCG GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA
3351 TCAAATTAAG CAGAAGGCCA TCCTGACGGA TGGCCTTTTT GCGTTTCTAC
3401 AAACTCTTTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
