pEpiFOS-5
At a Glance
pEpiFOS-5, Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
F-factor |
| Copy # |
single |
| Markers | |
| Link to Sequence | |
| Features |
Propagation of fosmid clones at single copy results in more clones and improved clone stability compared to cloning high-copy cosmids. [1] |
| Accession Number | |
| edit table |
</protect>
Notes
History
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Sources
References
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Sequence
1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
301 cagtgaattg taatacgact cactataggg cgaattcgag ctcggtaccc ggggatccca
361 cgtgggatcc tctagagtcg acctgcaggc atgcaagctt gagtattcta tagtctcacc
421 taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
481 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
541 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
601 cgtgccagct gcattaatga atcggccaac gcgaacccct tgcggccgcc cgggccgtcg
661 accaattctc atgtttgaca gcttatcatc gaatttctgc cattcatccg cttattatca
721 cttattcagg cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac
781 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
841 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
901 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
961 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
1021 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
1081 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
1141 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
1201 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
1261 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
1321 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
1381 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
1441 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
1501 tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag
1561 ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga
1621 tcttccgtca caggtattta ttcgcgataa gctcatggag cggcgtaacc gtcgcacagg
1681 aaggacagag aaagcgcgga tctgggaagt gacggacaga acggtcagga cctggattgg
1741 ggaggcggtt gccgccgctg ctgctgacgg tgtgacgttc tctgttccgg tcacaccaca
1801 tacgttccgc cattcctatg cgatgcacat gctgtatgcc ggtataccgc tgaaagttct
1861 gcaaagcctg atgggacata agtccatcag ttcaacggaa gtctacacga aggtttttgc
1921 gctggatgtg gctgcccggc accgggtgca gtttgcgatg ccggagtctg atgcggttgc
1981 gatgctgaaa caattatcct gagaataaat gccttggcct ttatatggaa atgtggaact
2041 gagtggatat gctgtttttg tctgttaaac agagaagctg gctgttatcc actgagaagc
2101 gaacgaaaca gtcgggaaaa tctcccatta tcgtagagat ccgcattatt aatctcagga
2161 gcctgtgtag cgtttatagg aagtagtgtt ctgtcatgat gcctgcaagc ggtaacgaaa
2221 acgatttgaa tatgccttca ggaacaatag aaatcttcgt gcggtgttac gttgaagtgg
2281 agcggattat gtcagcaatg gacagaacaa cctaatgaac acagaaccat gatgtggtct
2341 gtccttttac agccagtagt gctcgccgca gtcgagcgac agggcgaagc cctcgagtga
2401 gcgaggaagc accagggaac agcacttata tattctgctt acacacgatg cctgaaaaaa
2461 cttcccttgg ggttatccac ttatccacgg ggatattttt ataattattt tttttatagt
2521 ttttagatct tcttttttag agcgccttgt aggcctttat ccatgctggt tctagagaag
2581 gtgttgtgac aaattgccct ttcagtgtga caaatcaccc tcaaatgaca gtcctgtctg
2641 tgacaaattg cccttaaccc tgtgacaaat tgccctcaga agaagctgtt ttttcacaaa
2701 gttatccctg cttattgact cttttttatt tagtgtgaca atctaaaaac ttgtcacact
2761 tcacatggat ctgtcatggc ggaaacagcg gttatcaatc acaagaaacg taaaaatagc
2821 ccgcgaatcg tccagtcaaa cgacctcact gaggcggcat atagtctctc ccgggatcaa
2881 aaacgtatgc tgtatctgtt cgttgaccag atcagaaaat ctgatggcac cctacaggaa
2941 catgacggta tctgcgagat ccatgttgct aaatatgctg aaatattcgg attgacctct
3001 gcggaagcca gtaaggatat acggcaggca ttgaagagtt tcgcggggaa ggaagtggtt
3061 ttttatcgcc ctgaagagga tgccggcgat gaaaaaggct atgaatcttt tccttggttt
3121 atcaaacgtg cgcacagtcc atccagaggg ctttacagtg tacatatcaa cccatatctc
3181 attcccttct ttatcgggtt acagaaccgg tttacgcagt ttcggcttag tgaaacaaaa
3241 gaaatcacca atccgtatgc catgcgttta tacgaatccc tgtgtcagta tcgtaagccg
3301 gatggctcag gcatcgtctc tctgaaaatc gactggatca tagagcgtta ccagctgcct
3361 caaagttacc agcgtatgcc tgacttccgc cgccgcttcc tgcaggtctg tgttaatgag
3421 atcaacagca gaactccaat gcgcctctca tacattgaga aaaagaaagg ccgccagacg
3481 actcatatcg tattttcctt ccgcgatatc acttccatga cgacaggata gtctgagggt
3541 tatctgtcac agatttgagg gtggttcgtc acatttgttc tgacctactg agggtaattt
3601 gtcacagttt tgctgtttcc ttcagcctgc atggattttc tcatactttt tgaactgtaa
3661 tttttaagga agccaaattt gagggcagtt tgtcacagtt gatttccttc tctttccctt
3721 cgtcatgtga cctgatatcg ggggttagtt cgtcatcatt gatgagggtt gattatcaca
3781 gtttattact ctgaattggc tatccgcgtg tgtacctcta cctggagttt ttcccacggt
3841 ggatatttct tcttgcgctg agcgtaagag ctatctgaca gaacagttct tctttgcttc
3901 ctcgccagtt cgctcgctat gctcggttac acggctgcgg cgagcgctag tgataataag
3961 tgactgaggt atgtgctctt cttatctcct tttgtagtgt tgctcttatt ttaaacaact
4021 ttgcggtttt ttgatgactt tgcgattttg ttgttgcttt gcagtaaatt gcaagattta
4081 ataaaaaaac gcaaagcaat gattaaagga tgttcagaat gaaactcatg gaaacactta
4141 accagtgcat aaacgctggt catgaaatga cgaaggctat cgccattgca cagtttaatg
4201 atgacagccc ggaagcgagg aaaataaccc ggcgctggag aataggtgaa gcagcggatt
4261 tagttggggt ttcttctcag gctatcagag atgccgagaa agcagggcga ctaccgcacc
4321 cggatatgga aattcgagga cgggttgagc aacgtgttgg ttatacaatt gaacaaatta
4381 atcatatgcg tgatgtgttt ggtacgcgat tgcgacgtgc tgaagacgta tttccaccgg
4441 tgatcggggt tgctgcccat aaaggtggcg tttacaaaac ctcagtttct gttcatcttg
4501 ctcaggatct ggctctgaag gggctacgtg ttttgctcgt ggaaggtaac gacccccagg
4561 gaacagcctc aatgtatcac ggatgggtac cagatcttca tattcatgca gaagacactc
4621 tcctgccttt ctatcttggg gaaaaggacg atgtcactta tgcaataaag cccacttgct
4681 ggccggggct tgacattatt ccttcctgtc tggctctgca ccgtattgaa actgagttaa
4741 tgggcaaatt tgatgaaggt aaactgccca ccgatccaca cctgatgctc cgactggcca
4801 ttgaaactgt tgctcatgac tatgatgtca tagttattga cagcgcgcct aacctgggta
4861 tcggcacgat taatgtcgta tgtgctgctg atgtgctgat tgttcccacg cctgctgagt
4921 tgtttgacta cacctccgca ctgcagtttt tcgatatgct tcgtgatctg ctcaagaacg
4981 ttgatcttaa agggttcgag cctgatgtac gtattttgct taccaaatac agcaatagta
5041 atggctctca gtccccgtgg atggaggagc aaattcggga tgcctgggga agcatggttc
5101 taaaaaatgt tgtacgtgaa acggatgaag ttggtaaagg tcagatccgg atgagaactg
5161 tttttgaaca ggccattgat caacgctctt caactggtgc ctggagaaat gctctttcta
5221 tttgggaacc tgtctgcaat gaaattttcg atcgtctgat taaaccacgc tgggagatta
5281 gataatgaag cgtgcgcctg ttattccaaa acatacgctc aatactcaac cggttgaaga
5341 tacttcgtta tcgacaccag ctgccccgat ggtggattcg ttaattgcgc gcgtaggagt
5401 aatggctcgc ggtaatgcca ttactttgcc tgtatgtggt cgggatgtga agtttactct
5461 tgaagtgctc cggggtgata gtgttgagaa gacctctcgg gtatggtcag gtaatgaacg
5521 tgaccaggag ctgcttactg aggacgcact ggatgatctc atcccttctt ttctactgac
5581 tggtcaacag acaccggcgt tcggtcgaag agtatctggt gtcatagaaa ttgccgatgg
5641 gagtcgccgt cgtaaagctg ctgcacttac cgaaagtgat tatcgtgttc tggttggcga
5701 gctggatgat gagcagatgg ctgcattatc cagattgggt aacgattatc gcccaacaag
5761 tgcttatgaa cgtggtcagc gttatgcaag ccgattgcag aatgaatttg ctggaaatat
5821 ttctgcgctg gctgatgcgg aaaatatttc acgtaagatt attacccgct gtatcaacac
5881 cgccaaattg cctaaatcag ttgttgctct tttttctcac cccggtgaac tatctgcccg
5941 gtcaggtgat gcacttcaaa aagcctttac agataaagag gaattactta agcagcaggc
6001 atctaacctt catgagcaga aaaaagctgg ggtgatattt gaagctgaag aagttatcac
6061 tcttttaact tctgtgctta aaacgtcatc tgcatcaaga actagtttaa gctcacgaca
6121 tcagtttgct cctggagcga cagtattgta taagggcgat aaaatggtgc ttaacctgga
6181 caggtctcgt gttccaactg agtgtataga gaaaattgag gccattctta aggaacttga
6241 aaagccagca ccctgatgcg accacgtttt agtctacgtt tatctgtctt tacttaatgt
6301 cctttgttac aggccagaaa gcataactgg cctgaatatt ctctctgggc ccactgttcc
6361 acttgtatcg tcggtctgat aatcagactg ggaccacggt cccactcgta tcgtcggtct
6421 gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac
6481 cacggtccca ctcgtatcgt cggtctgata atcagactgg gaccacggtc ccactcgtat
6541 cgtcggtctg attattagtc tgggaccatg gtcccactcg tatcgtcggt ctgattatta
6601 gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctgga accacggtcc
6661 cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc
6721 tgattattag tctgggacca cgatcccact cgtgttgtcg gtctgattat cggtctggga
6781 ccacggtccc acttgtattg tcgatcagac tatcagcgtg agactacgat tccatcaatg
6841 cctgtcaagg gcaagtattg acatgtcgtc gtaacctgta gaacggagta acctcggtgt
6901 gcggttgtat gcctgctgtg gattgctgct gtgtcctgct tatccacaac attttgcgca
6961 cggttatgtg gacaaaatac ctggttaccc aggccgtgcc ggcacgttaa ccgggctgca
7021 tccgatgcaa gtgtgtcgct gtcgacgagc tcgcgagctc ggacatgagg ttgccccgta
7081 ttcagtgtcg ctgatttgta ttgtctgaag ttgtttttac gttaagttga tgcagatcaa
7141 ttaatacgat acctgcgtca taattgatta tttgacgtgg tttgatggcc tccacgcacg
7201 ttgtgatatg tagatgataa tcattatcac tttacgggtc ctttccggtg atccgacagg
7261 ttacggggcg gcgacctcgc gggttttcgc tatttatgaa aattttccgg tttaaggcgt
7321 ttccgttctt cttcgtcata acttaatgtt tttatttaaa ataccctctg aaaagaaagg
7381 aaacgacagg tgctgaaagc gagctttttg gcctctgtcg tttcctttct ctgtttttgt
7441 ccgtggaatg aacaatggaa gtccgagctc atcgctaata acttcgtata gcatacatta
7501 tacgaagtta tattcgat
