pET-9d
At a Glance
pET-9d Image made with PlasMapper
<protect>
| Type |
Cloning/Expression |
|---|---|
| Origin of Replication |
pBR322 |
| Copy # |
~40 |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number |
|
| edit table |
</protect>
Notes
pET-9d[1] is the same as pET-9a with the exception that pET-9b is 3bp shorter at 4338bp and within the MCS there is an NcoI site in place of the NdeI site; for the sites beyond 551 in pET-3a subtract 3bp.
History
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Sources
References
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Sequence
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
481 cagcttcctt tcgggctttg ttagcagccg gatccgaccc atttgctgtc caccagtcat
541 gctagccatg gtatatctcc ttcttaaagt taaacaaaat tatttctaga gggaaaccgt
601 tgtggtctcc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg
661 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
721 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
781 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
841 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
901 tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc aacccagtca
961 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
1021 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
1081 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
1141 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
1201 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
1261 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
1321 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
1381 cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg
1441 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct
1501 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg
1561 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag
1621 aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag
1681 cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt
1741 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga
1801 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc
1861 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc
1921 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
1981 acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg
2041 ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc
2101 atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa
2161 cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat
2221 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc
2281 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag
2341 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
2401 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
2461 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
2521 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt
2581 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
2641 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
2701 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
2761 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
2821 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
2881 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
2941 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
3001 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
3061 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
3121 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
3181 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
3241 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
3301 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
3361 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
3421 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgaacaata
3481 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
3541 acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
3601 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc
3661 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
3721 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
3781 atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc
3841 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
3901 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
3961 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
4021 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca
4081 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
4141 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
4201 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
4261 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
4321 gatgagtttt tctaagaa
