pET-9b
At a Glance
pET-9b Image made with PlasMapper
<protect>
| Type |
Cloning/Expression |
|---|---|
| Origin of Replication |
pBR322
|
| Copy # |
~40 |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number |
|
| edit table |
</protect>
Notes
pET-9b is the same as pET-9a with the exception that pET-9b is 1bp shorter at 4340bp; for the sites beyond BamH1 at 510 subtract 1bp.
History
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Sources
Novagen. Discontinued.
Promega. Discontinued.
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
481 cagcttcctt tcgggctttg ttagcagccg gatcccgacc catttgctgt ccaccagtca
541 tgctagccat atgtatatct ccttcttaaa gttaaacaaa attatttcta gagggaaacc
601 gttgtggtct ccctatagtg agtcgtatta atttcgcggg atcgagatct cgatcctcta
661 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
721 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
781 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
841 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
901 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt
961 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
1021 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
1081 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc
1141 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat
1201 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg
1261 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt
1321 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct
1381 cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc
1441 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt
1501 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc
1561 cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg
1621 agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc
1681 agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc
1741 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat
1801 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga
1861 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca
1921 gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga
1981 acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc
2041 cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca
2101 tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg
2161 aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac
2221 atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac
2281 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc
2341 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
2401 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
2461 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
2521 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatatgcg
2581 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
2641 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
2701 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
2761 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
2821 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
2881 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
2941 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
3001 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
3061 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
3121 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
3181 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
3241 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
3301 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
3361 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
3421 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgaacaa
3481 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
3541 aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
3601 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc
3661 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
3721 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
3781 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg aaaacagcat
3841 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
3901 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
3961 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
4021 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc
4081 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
4141 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
4201 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
4261 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
4321 tcgatgagtt tttctaagaa
