pET-44b(+)

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At a Glance

pET-44b(+), Image provided by Novagen

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Type

Expression and Cloning

Origin of Replication

f1
pBr322

Copy #

~40

Markers

Ampicillin

Link to Sequence

Novagen

Features

T7 Promoter
N and C Terminal His Tag
S Tag
Nus Tag
MCS
C Terminal HSV Tag
lacI

Accession Number


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Notes

The pET-44b(+) vector is specially designed for cloning and high-level expression of polypeptide sequences fused with the 495 aa NusA (Nus•Tag) protein. When E.coli cytoplasm is expressed as NusA fusions, the solubility of the various target protein in E.Coli cytoplasm is expressed. pET-43.1(+) vector series derived the pET-44(+) series which encodes an additional N-Terminal His•Tag sequence. When cloning into the PshA I or Sma I sites, the vector encoded sequence can be completely removed by the cleavage of the Nus•Tag fusion protein with enterokinase or thrombin, respectively. Note that although the vector map shows pET-44a(+), the map is still the same for pET-44b(+), with different expression sites.

History

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Sources

Novagen

References

See Help:References for how to manage references in EcoliWiki.


Sequence

       1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
      61 ggggttatgc tagttattgc tcagcggata catatttgaa tgtatttaga aaaataaaca 
     121 aaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttaatttgat 
     181 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcgcaacgt 
     241 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga 
     301 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc 
     361 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag 
     421 ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt ctgttttatc 
     481 agcctaggaa cgcccaactt aattaacatt agtggtggtg gtggtggtgc tcgagatcct 
     541 ctgggtcttc aggagcgagt tctggctggc ttgcacgtgt atacagctgt gcggccgcaa 
     601 gcttcgaacg cgtatcgatg gtaccgtcga cgtcctgcag gcgcgcctgt acagaattcg 
     661 gatccacgag ctccggactc ttgtcgtcgt catcaatcgt accagaaccc gcgctgcccc 
     721 gggggaccag accagttggc ggtggcgagt ccatgtgctg gcgttcaaat ttcgcagcag 
     781 cggtttcttt acccgcggag tgatggtgat ggtgatgacc agaaccacta gtcgcttcgt 
     841 caccgaacca gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca 
     901 acccttcgat atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa 
     961 cgccacgggc ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat 
    1021 cgtcagccgg tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg 
    1081 catttttagc acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca 
    1141 acagctcttt catcggcaca taggccaatt cttccagcgt cgagaagcct tcttctacca 
    1201 gaacagtcgc gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt 
    1261 gcgcttccgc ctgatgctta gcttgcaggt cgtcaacggt catcacgttg agttcccaac 
    1321 cgctcagttg cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccagat 
    1381 taccggcttc aacggcgatg tccatggtgt gtttatcttc atccaccacg atagaagcaa 
    1441 cgtctgccgg tgccattgcg ttaatcacga actgcgccgg gttatcatcc cacaggacga 
    1501 tatcgatacg ctcgccaccc agttcagtag acaccgcctg aacacgcgcg ccacgcatac 
    1561 ctacgcaagc acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac 
    1621 gagaacccgg atcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttctggca 
    1681 cttcaatacg gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg 
    1741 cgccacgcgc ttccgggcga acggaataga gcacgccacg aacgcggtcg ccagggcgga 
    1801 agttttcacg cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat 
    1861 ccagagagat gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt 
    1921 gttcacggaa ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa 
    1981 cctgttttgc cgtctgggta gtgatacggt caaaggtaac agactcaatc tgatcttcaa 
    2041 cgtaatcgcc caggttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct 
    2101 tggtcggctg ggtgacttca tcaacaacta accagcgacg gaaagtgtca aaatcaccgc 
    2161 ttttgcgatc gatctgtacg cggacgtcga tctcttgttc atattttttc tttgttgctg 
    2221 tcgccagcgc gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg 
    2281 atacggcttc aactacagcc aaaatttctt tgttcatact agaatggtga tggtggtgat 
    2341 ggctgctgcc catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga 
    2401 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc 
    2461 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt 
    2521 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct 
    2581 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg 
    2641 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact 
    2701 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca 
    2761 tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca 
    2821 gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt 
    2881 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa 
    2941 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg 
    3001 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt 
    3061 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt 
    3121 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc 
    3181 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg 
    3241 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca 
    3301 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat 
    3361 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc 
    3421 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg 
    3481 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca 
    3541 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca 
    3601 ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt agtgggatac gacgataccg 
    3661 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg 
    3721 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc 
    3781 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg 
    3841 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 
    3901 aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccggga 
    3961 tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 
    4021 atgactagca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt 
    4081 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa 
    4141 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg 
    4201 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc 
    4261 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg 
    4321 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt 
    4381 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt 
    4441 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa 
    4501 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa 
    4561 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct 
    4621 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 
    4681 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 
    4741 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt 
    4801 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag 
    4861 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 
    4921 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 
    4981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 
    5041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 
    5101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 
    5161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 
    5221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 
    5281 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 
    5341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 
    5401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 
    5461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 
    5521 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 
    5581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 
    5641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 
    5701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 
    5761 tttggtcatg acctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 
    5821 gacaataacc ctgataaatg cttcaatcat gattgaaaaa ggaagagtat gagtattcaa 
    5881 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 
    5941 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 
    6001 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 
    6061 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 
    6121 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 
    6181 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 
    6241 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 
    6301 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 
    6361 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 
    6421 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 
    6481 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 
    6541 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 
    6601 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 
    6661 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 
    6721 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 
    6781 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgcc atcgtgccgc 
    6841 cagaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 
    6901 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 
    6961 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 
    7021 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 
    7081 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 
    7141 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 
    7201 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 
    7261 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca