pET-44 Ek/LIC
At a Glance
pET-44 Ek/LIC, Image made with PlasMapper
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| Type |
Cloning, Expression |
|---|---|
| Origin of Replication |
pBR322, f1 |
| Copy # |
High |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number |
|
| edit table |
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Notes
The pET-44 Ek/LIC Vector is prepared for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides fused with N-terminal His•Tag®,Nus•Tag™ and S•Tag™ sequences. Inserts can cloned without the need for restriction digestion or ligation.
History
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Sources
References
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Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggata catatttgaa tgtatttaga aaaataaaca 121 aaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttaatttgat 181 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcgcaacgt 241 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga 301 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc 361 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag 421 ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt ctgttttatc 481 agcctaggaa cgcccaactt aattaacatt agtggtggtg gtggtggtgc tcgagatcct 541 ctgggtcttc aggagcgagt tctggctggc ttgcacgtgt atacagctgt gcggccgcaa 601 gcttcgaacg cgtatcgatg gtaccgtcga cgtcctgcag gcgcgcctgt acagaattcg 661 gatccacgag ctcagatatc gttgaggaga agcccggact cttgtcgtcg tcatcaatcg 721 taccagaacc cgcgctgccc cgggggacca gaccagttgg cggtggcgag tccatgtgct 781 ggcgttcaaa tttcgcagca gcggtttctt tacccgcgga gtgatggtga tggtgatgac 841 cagaaccact agtcgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg 901 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg 961 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta 1021 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg 1081 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct 1141 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccaat tcttccagcg 1201 tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg 1261 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcttgcagg tcgtcaacgg 1321 tcatcacgtt gagttcccaa ccgctcagtt gcgaagccag acgcacgttc tgaccgttac 1381 ggccaatcgc ctgcgccaga ttaccggctt caacggcgat gtccatggtg tgtttatctt 1441 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcacg aactgcgccg 1501 ggttatcatc ccacaggacg atatcgatac gctcgccacc cagttcagta gacaccgcct 1561 gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg 1621 ttttcaccgc gattttcgca cgagaacccg gatcgcgagc cgctgcttta atttcaatca 1681 cttcttcgcc gatttctggc acttcaatac ggaacagttc gatcagcatt tccggcttgg 1741 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaatag agcacgccac 1801 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg 1861 cttcagcgtt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc 1921 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt 1981 cacgcacttt ctgcacgata acctgttttg ccgtctgggt agtgatacgg tcaaaggtaa 2041 cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg 2101 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac 2161 ggaaagtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg atctcttgtt 2221 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc 2281 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatac 2341 tagaatggtg atggtggtga tggctgctgc ccatatgtat atctccttct taaagttaaa 2401 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat 2461 taatttcgcg ggatcgagat cgatctcgat cctctacgcc ggacgcatcg tggccggcat 2521 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga 2581 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 2641 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt 2701 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga 2761 gcgtcgagat cccggacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc 2821 gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg 2881 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg 2941 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca 3001 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca 3061 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac 3121 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg 3181 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg 3241 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg 3301 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg 3361 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa 3421 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa 3481 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca 3541 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg 3601 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gacatctcgg 3661 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca 3721 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg 3781 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc 3841 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 3901 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag 3961 ctcactcatt aggcaccggg atctcgaccg atgcccttga gagccttcaa cccagtcagc 4021 tccttccggt gggcgcgggg catgactagc atgatcgtgc tcctgtcgtt gaggacccgg 4141 tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt 4201 ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg 4261 atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag 4321 cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt 4381 ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc 4441 atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag 4501 gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag 4561 acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt 4621 gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat 4681 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 4741 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 4801 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat 4861 cagagcagat tgtactgaga gtgcaccata tatgcggtgt gaaataccgc acagatgcgt 4921 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 4981 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5041 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 5101 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 5161 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 5221 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 5281 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 5341 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 5401 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 5461 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 5521 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 5581 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 5641 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 5701 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 5761 cgaaaactca cgttaaggga ttttggtcat gacctatttg tttatttttc taaatacatt 5821 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaatca tgattgaaaa 5881 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 5941 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 6001 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 6061 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 6121 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 6181 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 6241 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 6301 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 6421 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 6481 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 6541 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 6601 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 6661 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 6721 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 6781 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 6841 aatctcatgc catcgtgccg ccagaaattg taaacgttaa tattttgtta aaattcgcgt 6901 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt 6961 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc 7021 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 7081 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac 7141 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 7201 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 7261 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt 7321 cccattcgcc a
