pET-42b(+)
At a Glance
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pET-42b(+), Image provided by Novagen
<protect>
| Type |
Cloning, Expression |
|---|---|
| Origin of Replication |
f1 |
| Copy # |
~40 |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number |
|
| edit table |
</protect>
Notes
The pET-42b(+) has the popular GST fusion tag for enhanced production and solubility.
The pET-42 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The T7 expression region is reversed on the vector map due to the sequencing numbered by the pBR322 convention. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, T7 terminator primer should be used for single-stranded sequencing. When cloning into the PshaI site, the vector encoded sequence can be removed completely followed by the cleaving of the GST fusion protein with Factor Xa.
History
Sources
References
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc
241 ggatccccga tatccatgga cccgcgtccc tcaataccgg agccaccacc ggtacccaga
301 tctgggctgt ccatgtgctg gcgttcgaat ttagcagcag cggtttcttt cataccaatt
361 gcagtactac cgcgtggcac cagacccgcg gagtgatggt gatggtgatg accagaacca
421 ctagttgaac catccgattt tggaggatgg tcgccaccac caaacgtggc ttgccagccc
481 tgcaaaggcc atgctatata cttgctggat ttcaagtact tatcaatttg tgggatagct
541 tcaatacgtt ttttaaaaca aactaatttt gggaacgcat ccaggcacat tgggtccatg
601 tataaaacaa catcaagagc gtcatacaac atgaagtcag gatgggttac atgatcacca
661 tttaaatatg ttttatgaca taaacgatct tcgaacattt tcagcatttc aggtagcttg
721 ctaagaaaat caactttgag agtttcaaag tctttactat atgcaattct cgaaacaccg
781 tatctaatat ccaaaaccgc tccttcaagc attgaaatct ctgcacgctc ttttggacaa
841 ccacccaaca tgttgtgctt gtcagctata taacgtatga tggccataga ctgtgttaat
901 ttaacatcac catcaatata ataaggaaga ttgggaaact ccaaacccaa ttcaaacttt
961 ttgtttcgcc atttatcacc ttcatcgcgc tcatacaaat gctcttcata tttttcttca
1021 agatattcca aaagaagtcg agtgggttgc acaaggccct taattttcca ataacctagt
1081 ataggggaca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat
1141 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatcga
1201 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
1261 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
1321 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
1381 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
1441 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc
1501 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
1561 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
1621 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
1681 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
1741 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
1801 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
1861 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
1921 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
1981 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
2041 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
2101 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
2161 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
2221 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
2281 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
2341 accgagtccg ggctgcgcgt tggtgcggac atctcggtag tgggatacga cgataccgaa
2401 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
2461 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
2521 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
2581 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
2641 agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc
2701 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
2761 gactagcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac
2821 tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac
2881 gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga
2941 aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg
3001 gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat
3061 ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa
3121 ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat
3181 cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa
3241 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact
3301 caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga
3361 tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
3421 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
3481 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
3541 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
3601 caccatatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
3661 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
3721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
3781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
3841 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
3901 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
3961 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
4021 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
4081 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
4141 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
4201 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
4261 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
4321 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
4381 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
4441 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
4501 tggtcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
4561 catattcaac gggaaacgtc ttgctctagg ccgcgattaa attccaacat ggatgctgat
4621 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
4681 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
4741 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
4801 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
4861 gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
4921 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
4981 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
5041 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
5101 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
5161 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
5221 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
5281 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
5341 tttcatttga tgctcgatga gtttttctaa gaattaattc atgagcggat acatatttga
5401 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
5461 tgaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
5521 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
5581 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
5641 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
5701 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
5761 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
5821 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
5881 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgcca
