pET-41a(+)
At a Glance
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pET-41a(+), Image provided by Novagen
<protect>
| Type |
Cloning, Expression |
|---|---|
| Origin of Replication |
f1 |
| Copy # |
~40 |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number |
|
| edit table |
</protect>
Notes
pET-41a(+) has the popular GST fusion tag for enhanced production and solubility.
The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is included. The T7 expression region is reversed on the vector map due to the sequencing numbered by the pBR322 convention. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, T7 terminator primer should be used for single-stranded sequencing. When cloning into the PshaI site,the vector encoded sequence can be removed completely followed by the cleaving of the GST fusion protein with Enterokinase.
History
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Sources
References
[1] [2] [3] [4] [5] [6] [7] [8] [9] [10]
- ↑ Kulman, JD et al. (2007) Proline-rich Gla protein 2 is a cell-surface vitamin K-dependent protein that binds to the transcriptional coactivator Yes-associated protein. Proc. Natl. Acad. Sci. U.S.A. 104 8767-72 PubMed
- ↑ Beernink, PT et al. (2005) Specificity of protein interactions mediated by BRCT domains of the XRCC1 DNA repair protein. J. Biol. Chem. 280 30206-13 PubMed
- ↑ Chellappan, P et al. (2005) MicroRNA-binding viral protein interferes with Arabidopsis development. Proc. Natl. Acad. Sci. U.S.A. 102 10381-6 PubMed
- ↑ Fotso, P et al. (2005) Cog1p plays a central role in the organization of the yeast conserved oligomeric Golgi complex. J. Biol. Chem. 280 27613-23 PubMed
- ↑ Li, L et al. (2005) Clarification of cinnamoyl co-enzyme A reductase catalysis in monolignol biosynthesis of Aspen. Plant Cell Physiol. 46 1073-82 PubMed
- ↑ Liedtke, CM et al. (2005) Role for protein phosphatase 2A in the regulation of Calu-3 epithelial Na+-K+-2Cl-, type 1 co-transport function. J. Biol. Chem. 280 25491-8 PubMed
- ↑ Madsen, KL et al. (2005) Molecular determinants for the complex binding specificity of the PDZ domain in PICK1. J. Biol. Chem. 280 20539-48 PubMed
- ↑ Srivastava, SK et al. (2005) NAD+-dependent DNA Ligase (Rv3014c) from Mycobacterium tuberculosis. Crystal structure of the adenylation domain and identification of novel inhibitors. J. Biol. Chem. 280 30273-81 PubMed
- ↑ Yang, HY et al. (2005) Kinesin-1 mediates translocation of the meiotic spindle to the oocyte cortex through KCA-1, a novel cargo adapter. J. Cell Biol. 169 447-57 PubMed
- ↑ Zong, X et al. (2005) A novel mechanism of modulation of hyperpolarization-activated cyclic nucleotide-gated channels by Src kinase. J. Biol. Chem. 280 34224-32 PubMed
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc
241 ggatccccga tatcccatgg gactcttgtc gtcgtcatca ccggagccac caccggtacc
301 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatacc
361 aattgcagta ctaccgcgtg gcaccagacc cgcggagtga tggtgatggt gatgaccaga
421 accactagtt gaaccatccg attttggagg atggtcgcca ccaccaaacg tggcttgcca
481 gccctgcaaa ggccatgcta tatacttgct ggatttcaag tacttatcaa tttgtgggat
541 agcttcaata cgttttttaa aacaaactaa ttttgggaac gcatccaggc acattgggtc
601 catgtataaa acaacatcaa gagcgtcata caacatgaag tcaggatggg ttacatgatc
661 accatttaaa tatgttttat gacataaacg atcttcgaac attttcagca tttcaggtag
721 cttgctaaga aaatcaactt tgagagtttc aaagtcttta ctatatgcaa ttctcgaaac
781 accgtatcta atatccaaaa ccgctccttc aagcattgaa atctctgcac gctcttttgg
841 acaaccaccc aacatgttgt gcttgtcagc tatataacgt atgatggcca tagactgtgt
901 taatttaaca tcaccatcaa tataataagg aagattggga aactccaaac ccaattcaaa
961 ctttttgttt cgccatttat caccttcatc gcgctcatac aaatgctctt catatttttc
1021 ttcaagatat tccaaaagaa gtcgagtggg ttgcacaagg cccttaattt tccaataacc
1081 tagtataggg gacatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg
1141 gaattgttat ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga
1201 tcgatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
1261 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
1321 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
1381 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
1441 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac
1501 catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
1561 cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
1621 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
1681 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
1741 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
1801 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
1861 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
1921 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
1981 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
2041 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
2101 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
2161 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
2221 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
2281 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
2341 cattaccgag tccgggctgc gcgttggtgc ggacatctcg gtagtgggat acgacgatac
2401 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
2461 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
2521 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
2581 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
2641 ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcaccgg
2701 gatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
2761 gcatgactag catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
2821 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
2881 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
2941 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
3001 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
3061 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
3121 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
3181 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
3241 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
3301 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
3361 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
3421 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
3481 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
3541 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
3601 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
3661 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
3721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
3781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
3841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
3901 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
3961 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
4021 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
4081 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
4141 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
4201 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
4261 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
4321 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
4381 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
4441 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
4501 attttggtca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
4561 gagccatatt caacgggaaa cgtcttgctc taggccgcga ttaaattcca acatggatgc
4621 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
4681 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
4741 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
4801 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
4861 ccccgggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
4921 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
4981 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
5041 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
5101 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
5161 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
5221 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
5281 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
5341 gcagtttcat ttgatgctcg atgagttttt ctaagaatta attcatgagc ggatacatat
5401 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
5461 cacctgaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
5521 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
5581 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
5641 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
5701 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
5761 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
5821 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
5881 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg cca
