pET-39b(+)
At a Glance
<protect>
Type |
Expression |
---|---|
Origin of Replication |
pBR322 |
Copy # |
~40 |
Markers |
Kanamycin |
Link to Sequence | |
Features |
T7 Promoter |
Accession Number |
|
edit table |
</protect>
Notes
pET-39b(+) is designed for expression of DsbA fusion proteins.
History
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Sources
References
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt 181 gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg atatcgccat ggttgaggag 241 aagcccgggc tcttgtcgtc gtcatcggta cccagatctg ggctgtccat gtgctggcgt 301 tcgaatttag cagcagcggt ttctttcata ccaattgcag tactaccgcg tggcaccaga 361 cccgcggagt gatggtgatg gtgatgacca gaaccactag ttgatccttt tttctcgctt 421 aagtatttca ctgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata 481 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacgcc acgcaattgc 541 acgtcagctg cagctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac 601 gccgcgtcgt actcttcacc tttaatacct gcgttgataa atacatcgcg gatatcagaa 661 gcagaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct 721 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca 781 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca 841 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa 901 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca 961 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa 1021 atctttttca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat 1081 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatcga 1141 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg 1201 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca 1261 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg 1321 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac 1381 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc 1441 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg 1501 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 1561 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 1621 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 1681 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 1741 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 1801 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 1861 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 1921 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 1981 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 2041 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 2101 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 2161 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 2221 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 2281 accgagtccg ggctgcgcgt tggtgcggac atctcggtag tgggatacga cgataccgaa 2341 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 2401 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 2461 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 2521 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 2581 agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc 2641 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat 2701 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc 2761 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg 2821 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc 2881 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccccacgggt 2941 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg 3001 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc 3061 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac 3121 gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct 3181 accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt 3241 tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg 3301 ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat 3361 tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac 3421 cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa 3481 cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga 3541 gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 3601 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 3661 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga 3721 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac 3781 catatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct 3841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 3901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 3961 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 4021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 4081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 4141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 4201 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 4261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 4321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 4381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 4441 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 4501 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 4561 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 4621 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 4681 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat 4741 attcaacggg aaacgtcttg ctctaggccg cgattaaatt ccaacatgga tgctgattta 4801 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg 4861 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat 4921 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc 4981 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg 5041 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg 5101 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc 5161 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg 5221 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat 5281 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac 5341 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca 5401 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta 5461 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt 5521 catttgatgc tcgatgagtt tttctaagaa ttaattcatg agcggataca tatttgaatg 5581 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 5641 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 5701 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 5761 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 5821 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 5881 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 5941 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 6001 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 6061 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca