pET-32c(+) DNA
At a Glance
![](/colipedia/images/thumb/a/a7/PET-32c%28%2B%29.jpg/450px-PET-32c%28%2B%29.jpg)
pET-32c(+), Image made with PlasMapper
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Type |
Cloning, Expression[1] |
---|---|
Origin of Replication |
pBR322, f1 |
Copy # | |
Markers |
Ampicillin |
Link to Sequence | |
Features |
T7 Promoter |
Accession Number |
|
edit table |
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Notes
pET-32c(+) is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag thioredoxin protein[1]. The map for pET-32c(+) is the same as pET-32a(+) except that pET-32c(+) is a 5901 bp plasmid; add 1bp to each site beyond BamHI at 198.
History
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Sources
References
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccacagata tcccatggcc ttgtcgtcgt cgtcggtacc 241 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatacc 301 agaaccgcgt ggcaccagac cagaagaatg atgatgatga tggtgcatat ggccagaacc 361 agaaccggcc aggttagcgt cgaggaactc tttcaactga cctttagaca gtgcacccac 421 tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac cacggatgcc 481 atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc 541 ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg gaccgcacca 601 ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg tgtcaaaact 661 gtcgtcagtc aggtgaataa ttttatcgct catatgtata tctccttctt aaagttaaac 721 aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt 781 aatttcgcgg gatcgagatc gatctcgatc ctctacgccg gacgcatcgt ggccggcatc 841 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat 901 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc 961 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg 1021 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag 1081 cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg 1141 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc 1201 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt 1261 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa 1321 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag 1381 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact 1441 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc 1501 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga 1561 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt 1621 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg 1681 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag 1741 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat 1801 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat 1861 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc 1921 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt 1981 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa 2041 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg 2101 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct 2161 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 2221 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc 2281 tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac ccagtcagct 2341 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca 2401 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc 2461 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg 2521 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg 2581 ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag 2641 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa 2701 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc 2761 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct 2821 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct 2881 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac 2941 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc 3001 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat 3061 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat 3121 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat 3181 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg 3241 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 3301 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 3361 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 3421 gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg 3481 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 3541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 3601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 3661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 3721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 3781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 3841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 3901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 3961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 4021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 4081 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 4141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 4201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 4261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 4321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 4381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 4441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 4501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 4561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 4621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 4681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 4741 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 4801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 4861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 4921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 4981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 5041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 5101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 5161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 5221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 5281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 5341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 5401 gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa tattttgtta 5461 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 5521 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 5581 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 5641 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 5701 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 5761 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 5821 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 5881 cagggcgcgt cccattcgcc a