pET-29a(+)
At a Glance
.JPG)
pET-29a-c(+), image provided by Novagen
<protect>
| Type |
Cloning, Expression |
|---|---|
| Origin of Replication |
f1 |
| Copy # |
~40 |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number |
|
| edit table |
</protect>
Notes
The pET-29a(+) vector carries an N-terminal S•Tag™/thrombin configuration including an optional C-terminal His•Tag® sequence. The T7 expression region is reversed on the vector map due to the sequencing numbered by the pBR322 convention. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, T7 terminator primer should be used for single-stranded sequencing.
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
References
[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15]
- ↑ Grillo, C et al. (2007) DNA-binding activity of the ERp57 C-terminal domain is related to a redox-dependent conformational change. J. Biol. Chem. 282 10299-310 PubMed
- ↑ Kulikowicz, T & Shapiro, TA (2006) Distinct genes encode type II Topoisomerases for the nucleus and mitochondrion in the protozoan parasite Trypanosoma brucei. J. Biol. Chem. 281 3048-56 PubMed
- ↑ Beernink, PT et al. (2005) Specificity of protein interactions mediated by BRCT domains of the XRCC1 DNA repair protein. J. Biol. Chem. 280 30206-13 PubMed
- ↑ Guazzaroni, ME et al. (2005) The multidrug efflux regulator TtgV recognizes a wide range of structurally different effectors in solution and complexed with target DNA: evidence from isothermal titration calorimetry. J. Biol. Chem. 280 20887-93 PubMed
- ↑ Lara, MV et al. (2005) NADP-malic enzyme and Hsp70: co-purification of both proteins and modification of NADP-malic enzyme properties by association with Hsp70. Plant Cell Physiol. 46 997-1006 PubMed
- ↑ Lin, PH & Lin-Chao, S (2005) RhlB helicase rather than enolase is the beta-subunit of the Escherichia coli polynucleotide phosphorylase (PNPase)-exoribonucleolytic complex. Proc. Natl. Acad. Sci. U.S.A. 102 16590-5 PubMed
- ↑ Lovering, AL et al. (2005) Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate. J. Biol. Chem. 280 2105-15 PubMed
- ↑ Nagaike, T et al. (2005) Human mitochondrial mRNAs are stabilized with polyadenylation regulated by mitochondria-specific poly(A) polymerase and polynucleotide phosphorylase. J. Biol. Chem. 280 19721-7 PubMed
- ↑ Rho, SB et al. (2005) The identification of apoptosis-related residues in human thymosin beta-10 by mutational analysis and computational modeling. J. Biol. Chem. 280 34003-7 PubMed
- ↑ Rider, SD Jr et al. (2005) The protozoan parasite Cryptosporidium parvum possesses two functionally and evolutionarily divergent replication protein A large subunits. J. Biol. Chem. 280 31460-9 PubMed
- ↑ Rancour, DM et al. (2004) Plant UBX domain-containing protein 1, PUX1, regulates the oligomeric structure and activity of arabidopsis CDC48. J. Biol. Chem. 279 54264-74 PubMed
- ↑ Gaynutdinov, TI et al. (2003) Chimeric ribonuclease as a source of human adapter protein for targeted drug delivery. Protein Eng. 16 771-5 PubMed
- ↑ Chazalet, V et al. (2001) Identification of essential amino acids in the Azorhizobium caulinodans fucosyltransferase NodZ. J. Bacteriol. 183 7067-75 PubMed
- ↑ Jia, Y et al. (2000) Direct interaction of resistance gene and avirulence gene products confers rice blast resistance. EMBO J. 19 4004-14 PubMed
- ↑ Ma, Z et al. (1999) Human pancreatic islets express mRNA species encoding two distinct catalytically active isoforms of group VI phospholipase A2 (iPLA2) that arise from an exon-skipping mechanism of alternative splicing of the transcript from the iPLA2 gene on chromosome 22q13.1. J. Biol. Chem. 274 9607-16 PubMed
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgatatca gccatggaac cgcgtggcac cagggtaccc
241 agatctgggc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc tttcatatgt
301 atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca
361 attcccctat agtgagtcgt attaatttcg cgggatcgag atcgatctcg atcctctacg
421 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
481 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
541 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
601 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
661 tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc
721 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
781 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
841 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
901 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
961 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
1021 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
1081 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
1141 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
1201 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
1261 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
1321 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
1381 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
1441 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
1501 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
1561 cgcgttggtg cggacatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
1621 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
1681 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
1741 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
1801 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
1861 caacgcaatt aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt
1921 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
1981 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt
2041 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt
2101 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
2161 cggcgagaag caggccatta tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct
2221 cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc
2281 accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac
2341 aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc
2401 ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc
2461 tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg
2521 catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc
2581 agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag
2641 aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc
2701 ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga
2761 tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg
2821 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
2881 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
2941 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
3001 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat atgcggtgtg
3061 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
3121 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
3181 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
3241 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
3301 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
3361 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
3421 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
3481 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
3541 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
3601 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
3661 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
3721 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
3781 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
3841 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
3901 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg aacaataaaa
3961 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
4021 tcttgctcta ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
4081 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat
4141 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
4201 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
4261 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag
4321 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
4381 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
4441 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
4501 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
4561 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
4621 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
4681 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
4741 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
4801 gagtttttct aagaattaat tcatgagcgg atacatattt gaatgtattt agaaaaataa
4861 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa
4921 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
4981 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
5041 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
5101 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
5161 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
5221 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
5281 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
5341 tgcgccgcta cagggcgcgt cccattcgcc a
