pET-26b(+)
At a Glance

pET-26b(+), Image provided by Novagen
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Type |
Expression |
---|---|
Origin of Replication |
f1 |
Copy # |
~40 |
Markers |
Kanamycin |
Link to Sequence | |
Features |
C terminal His Tag |
Accession Number |
|
edit table |
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Notes
The pET-26b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization in addition to an optional C-terminal His•Tag sequence. The T7 expression region is reversed on the vector map due to the sequencing numbered by the pBR322 convention. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, T7 terminator primer should be used for single-stranded sequencing.
History
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Sources
References
[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12]
- ↑ Funa, N et al. (2007) Pentaketide resorcylic acid synthesis by type III polyketide synthase from Neurospora crassa. J. Biol. Chem. 282 14476-81 PubMed
- ↑ Li, Y et al. (2007) Processivity, substrate binding, and mechanism of cellulose hydrolysis by Thermobifida fusca Cel9A. Appl. Environ. Microbiol. 73 3165-72 PubMed
- ↑ Heraud, JM et al. (2006) Subunit recombinant vaccine protects against monkeypox. J. Immunol. 177 2552-64 PubMed
- ↑ Brouns, SJ et al. (2005) Engineering a selectable marker for hyperthermophiles. J. Biol. Chem. 280 11422-31 PubMed
- ↑ Funa, N et al. (2005) A novel quinone-forming monooxygenase family involved in modification of aromatic polyketides. J. Biol. Chem. 280 14514-23 PubMed
- ↑ Ishii, R et al. (2005) Crystal structure of the tRNA 3' processing endoribonuclease tRNase Z from Thermotoga maritima. J. Biol. Chem. 280 14138-44 PubMed
- ↑ Li, H et al. (2005) Crystal structures of T cell receptor (beta) chains related to rheumatoid arthritis. Protein Sci. 14 3025-38 PubMed
- ↑ Tsubouchi, T et al. (2005) Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus. J. Biol. Chem. 280 18511-6 PubMed
- ↑ Vincent, F et al. (2005) Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily? J. Biol. Chem. 280 19649-55 PubMed
- ↑ Chen, YH et al. (2004) Structural basis of the alpha1-beta subunit interaction of voltage-gated Ca2+ channels. Nature 429 675-80 PubMed
- ↑ Teartasin, W et al. (2004) Expression, purification and preliminary X-ray diffraction analysis of a ketoreductase from a type II polyketide synthase. Acta Crystallogr. D Biol. Crystallogr. 60 1137-8 PubMed
- ↑ Goldberg, SD et al. (2003) Identification of residues critical for catalysis in a class C beta-lactamase by combinatorial scanning mutagenesis. Protein Sci. 12 1633-45 PubMed
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg 241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc 301 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc 361 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc 421 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc 481 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg 541 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt 601 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg 661 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat 721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt 781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca 841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa 901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt 961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg 1021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc 1081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta 1141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt 1201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg 1261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc 1321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac 1381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt 1441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa 1501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc 1561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt 1621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca 1681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag 1741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 1861 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca 1921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg 1981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg 2041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct 2101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc 2161 aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga 2221 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg 2281 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg 2341 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta 2401 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat 2461 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg 2521 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta 2581 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt 2641 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca 2701 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag 2761 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt 2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 2941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 3001 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac 3061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta 3961 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag 4021 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa 4081 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt 4141 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact 4201 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga 4261 tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga 4321 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca 4381 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc 4441 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg 4501 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc 4561 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat 4621 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct 4681 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg 4741 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta 4801 agaattaatt catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 4861 ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa 4921 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 4981 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 5041 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 5101 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 5161 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 5221 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 5281 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 5341 agggcgcgtc ccattcgcca