pET-26b(+)
At a Glance
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pET-26b(+), Image provided by Novagen
<protect>
| Type |
Expression |
|---|---|
| Origin of Replication |
f1 |
| Copy # |
~40 |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
C terminal His Tag |
| Accession Number |
|
| edit table |
</protect>
Notes
The pET-26b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization in addition to an optional C-terminal His•Tag sequence. The T7 expression region is reversed on the vector map due to the sequencing numbered by the pBR322 convention. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, T7 terminator primer should be used for single-stranded sequencing.
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
References
[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12]
- ↑ Funa, N et al. (2007) Pentaketide resorcylic acid synthesis by type III polyketide synthase from Neurospora crassa. J. Biol. Chem. 282 14476-81 PubMed
- ↑ Li, Y et al. (2007) Processivity, substrate binding, and mechanism of cellulose hydrolysis by Thermobifida fusca Cel9A. Appl. Environ. Microbiol. 73 3165-72 PubMed
- ↑ Heraud, JM et al. (2006) Subunit recombinant vaccine protects against monkeypox. J. Immunol. 177 2552-64 PubMed
- ↑ Brouns, SJ et al. (2005) Engineering a selectable marker for hyperthermophiles. J. Biol. Chem. 280 11422-31 PubMed
- ↑ Funa, N et al. (2005) A novel quinone-forming monooxygenase family involved in modification of aromatic polyketides. J. Biol. Chem. 280 14514-23 PubMed
- ↑ Ishii, R et al. (2005) Crystal structure of the tRNA 3' processing endoribonuclease tRNase Z from Thermotoga maritima. J. Biol. Chem. 280 14138-44 PubMed
- ↑ Li, H et al. (2005) Crystal structures of T cell receptor (beta) chains related to rheumatoid arthritis. Protein Sci. 14 3025-38 PubMed
- ↑ Tsubouchi, T et al. (2005) Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus. J. Biol. Chem. 280 18511-6 PubMed
- ↑ Vincent, F et al. (2005) Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily? J. Biol. Chem. 280 19649-55 PubMed
- ↑ Chen, YH et al. (2004) Structural basis of the alpha1-beta subunit interaction of voltage-gated Ca2+ channels. Nature 429 675-80 PubMed
- ↑ Teartasin, W et al. (2004) Expression, purification and preliminary X-ray diffraction analysis of a ketoreductase from a type II polyketide synthase. Acta Crystallogr. D Biol. Crystallogr. 60 1137-8 PubMed
- ↑ Goldberg, SD et al. (2003) Identification of residues critical for catalysis in a class C beta-lactamase by combinatorial scanning mutagenesis. Protein Sci. 12 1633-45 PubMed
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg
241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc
301 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc
361 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc
421 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
481 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
541 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
601 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
661 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat
721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
1021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
1081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
1141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
1201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
1261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
1321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
1381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
1441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
1501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
1561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
1621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
1681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
1741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
1861 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca
1921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
1981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
2041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
2101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
2161 aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga
2221 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
2281 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
2341 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta
2401 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
2461 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
2521 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
2581 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt
2641 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
2701 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
2761 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt
2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
2941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
3001 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac
3061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta
3961 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag
4021 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
4081 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt
4141 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
4201 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
4261 tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga
4321 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
4381 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
4441 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
4501 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
4561 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
4621 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
4681 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
4741 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
4801 agaattaatt catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
4861 ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa
4921 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
4981 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
5041 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
5101 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
5161 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
5221 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
5281 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
5341 agggcgcgtc ccattcgcca
