pET-24c(+)
At a Glance

<protect>
Type |
Expression |
---|---|
Origin of Replication |
f1, pBR322 |
Copy # |
~40 |
Markers |
Kanamycin |
Link to Sequence | |
Features |
T7 promoter |
Accession Number |
|
edit table |
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Notes
The pET-24a-d(+) vectors possess an N-terminal sequence that codes for a T7 Tag. It also comes with an optional C-terminal His Tag sequence. The only difference between each individual plasmid from a-d is the selectable marker. In pET-24b(+) the selectable marker is kanamycin. On the vector ap the T7 repression region is opposite a normal region meaning it is reversed. This is a characteristic of the BR322 numbering convention. When exposed to helper phage, the fl origin produces virions containing single-dtranded DNA. These single stranded DNA correspond to the coding strand. The virions are a product of the orientation of the f1 origin.
History
All pET-24a-d(+) plasmids have the same structure and vector map. They differ in their selectable markers.
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg 241 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac 301 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg 361 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg 421 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg 481 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac 541 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc 601 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt 661 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca 721 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 781 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 841 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 901 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 961 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 1021 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 1081 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 1141 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 1201 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 1261 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 1321 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 1381 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 1441 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 1501 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 1561 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 1621 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 1681 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 1741 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 1801 cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag 1861 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact 1921 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat 1981 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt 2041 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg 2101 cgagaagcag gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct 2161 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc 2221 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac 2281 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg 2341 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac 2401 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat 2461 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt 2521 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa 2581 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg 2641 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga 2701 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct 2761 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 2821 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 2881 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg 2941 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa 3001 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca 3061 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 3121 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 3181 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 3241 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 3301 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 3361 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 3421 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 3481 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 3541 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 3601 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 3661 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 3721 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 3781 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 3841 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg 3901 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct 3961 tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct 4021 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg 4081 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 4141 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt 4201 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta 4261 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc 4321 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc 4381 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag 4441 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca 4501 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 4561 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 4621 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 4681 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 4741 tttttctaag aattaattca tgagcggata catatttgaa tgtatttaga aaaataaaca 4801 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaaattgtaa acgttaatat 4861 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 4921 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 4981 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 5041 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 5101 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 5161 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 5221 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 5281 gccgctacag ggcgcgtccc attcgcca