pET-24b(+)
At a Glance
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pET-24b(+), Image made with PlasMapper
<protect>
| Type |
Expression |
|---|---|
| Origin of Replication |
f1, pBR322 |
| Copy # |
~40 |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 promoter |
| Accession Number |
|
| edit table |
</protect>
Notes
The pET-24a-d(+) vectors possess an N-terminal sequence that codes for a T7 Tag. It also comes with an optional C-terminal His Tag sequence. The only difference between each individual plasmid from a-d is the selectable marker. In pET-24b(+) the selectable marker is kanamycin. On the vector ap the T7 repression region is opposite a normal region meaning it is reversed. This is a characteristic of the BR322 numbering convention. When exposed to helper phage, the fl origin produces virions containing single-dtranded DNA. These single stranded DNA correspond to the coding strand. The virions are a product of the orientation of the f1 origin.
History
All pET-24a-d(+) plasmids have the same structure and vector map. The only difference is the selectable marker in each plasmid.
Sources
References
[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15]
See Help:References for how to manage references in EcoliWiki.
- ↑ Ikegami, A et al. (2007) The CHLI1 subunit of Arabidopsis thaliana magnesium chelatase is a target protein of the chloroplast thioredoxin. J. Biol. Chem. 282 19282-91 PubMed
- ↑ Leach, MR et al. (2007) The role of complex formation between the Escherichia coli hydrogenase accessory factors HypB and SlyD. J. Biol. Chem. 282 16177-86 PubMed
- ↑ Yuan, Y et al. (2007) Crystal structure of a peptidoglycan glycosyltransferase suggests a model for processive glycan chain synthesis. Proc. Natl. Acad. Sci. U.S.A. 104 5348-53 PubMed
- ↑ Breazeale, SD et al. (2005) A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-Amino-4-deoxy-L-arabinose. Identification and function oF UDP-4-deoxy-4-formamido-L-arabinose. J. Biol. Chem. 280 14154-67 PubMed
- ↑ Fischbach, MA et al. (2005) In vitro characterization of IroB, a pathogen-associated C-glycosyltransferase. Proc. Natl. Acad. Sci. U.S.A. 102 571-6 PubMed
- ↑ Fotso, P et al. (2005) Cog1p plays a central role in the organization of the yeast conserved oligomeric Golgi complex. J. Biol. Chem. 280 27613-23 PubMed
- ↑ Nakamura, Y et al. (2005) A novel phosphatidylcholine-hydrolyzing phospholipase C induced by phosphate starvation in Arabidopsis. J. Biol. Chem. 280 7469-76 PubMed
- ↑ Nita-Lazar, M et al. (2005) The N-X-S/T consensus sequence is required but not sufficient for bacterial N-linked protein glycosylation. Glycobiology 15 361-7 PubMed
- ↑ Oh, SW et al. (2005) JNK regulates lifespan in Caenorhabditis elegans by modulating nuclear translocation of forkhead transcription factor/DAF-16. Proc. Natl. Acad. Sci. U.S.A. 102 4494-9 PubMed
- ↑ Okada, K & Hase, T (2005) Cyanobacterial non-mevalonate pathway: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase interacts with ferredoxin in Thermosynechococcus elongatus BP-1. J. Biol. Chem. 280 20672-9 PubMed
- ↑ Paradis-Bleau, C et al. (2005) Peptide inhibitors of the essential cell division protein FtsA. Protein Eng. Des. Sel. 18 85-91 PubMed
- ↑ Suzuki, T et al. (2005) An Arabidopsis protein with a novel calcium-binding repeat sequence interacts with TONSOKU/MGOUN3/BRUSHY1 involved in meristem maintenance. Plant Cell Physiol. 46 1452-61 PubMed
- ↑ Wennerhold, J et al. (2005) The AraC-type regulator RipA represses aconitase and other iron proteins from Corynebacterium under iron limitation and is itself repressed by DtxR. J. Biol. Chem. 280 40500-8 PubMed
- ↑ Yanagi, K et al. (2005) Caenorhabditis elegans geminin homologue participates in cell cycle regulation and germ line development. J. Biol. Chem. 280 19689-94 PubMed
- ↑ Zhang, JW et al. (2005) A role for SlyD in the Escherichia coli hydrogenase biosynthetic pathway. J. Biol. Chem. 280 4360-6 PubMed
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat
241 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
301 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc
361 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
421 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
481 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
541 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
601 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt
661 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
721 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
781 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
841 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
901 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
961 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
1021 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
1081 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
1141 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
1201 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
1261 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
1321 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
1381 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
1441 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
1501 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
1561 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
1621 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
1681 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
1741 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
1801 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga
1861 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
1921 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
1981 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
2041 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
2101 gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg atcgtgctcc
2161 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
2221 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
2281 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct
2341 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta
2401 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca
2461 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag
2521 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa
2581 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc
2641 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
2701 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc
2761 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
2821 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
2881 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
2941 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat gcggtgtgaa
3001 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
3061 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
3121 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
3181 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
3241 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
3301 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
3361 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
3421 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
3481 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
3541 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
3601 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
3661 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
3721 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
3781 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
3841 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgaa caataaaact
3901 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
3961 ttgctctagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
4021 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc
4081 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
4141 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
4201 tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt
4261 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
4321 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
4381 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
4441 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
4501 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
4561 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
4621 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
4681 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
4741 gtttttctaa gaattaattc atgagcggat acatatttga atgtatttag aaaaataaac
4801 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta aacgttaata
4861 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
4921 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
4981 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
5041 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
5101 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
5161 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
5221 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
5281 cgccgctaca gggcgcgtcc cattcgcca
