pET-24a(+)
At a Glance
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pET-24a(+) Image made with PlasMapper
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| Type |
Expression |
|---|---|
| Origin of Replication |
f1, pBR322 |
| Copy # |
~40 |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 promoter |
| Accession Number |
|
| edit table |
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Notes
The pET-24a-d(+) vectors possess an N-terminal sequence that codes for a T7 Tag. It also comes with an optional C-terminal His Tag sequence. The only difference between each individual plasmid from a-d is the selectable marker. In pET-24a(+) the selectable marker is kanamycin. On the vector ap the T7 repression region is opposite a normal region meaning it is reversed. This is a characteristic of the BR322 numbering convention. When exposed to helper phage, the fl origin produces virions containing single-dtranded DNA. These single stranded DNA correspond to the coding strand. The virions are a product of the orientation of the f1 origin.
History
All pET-24a-d(+) plasmids are the same except for their selectable markers. (kanamycin vs. ampicillin)
Sources
References
[1] [2] [3] [4] [5] [6] [7] [8] [9] [10]
- ↑ Sukumar, N et al. (2007) Differential Bvg phase-dependent regulation and combinatorial role in pathogenesis of two Bordetella paralogs, BipA and BcfA. J. Bacteriol. 189 3695-704 PubMed
- ↑ Boanca, G et al. (2006) Uncoupling the enzymatic and autoprocessing activities of Helicobacter pylori gamma-glutamyltranspeptidase. J. Biol. Chem. 281 19029-37 PubMed
- ↑ Gakh, O et al. (2006) Mitochondrial iron detoxification is a primary function of frataxin that limits oxidative damage and preserves cell longevity. Hum. Mol. Genet. 15 467-79 PubMed
- ↑ Sosnovtsev, SV et al. (2006) Cleavage map and proteolytic processing of the murine norovirus nonstructural polyprotein in infected cells. J. Virol. 80 7816-31 PubMed
- ↑ Beharry, Z & Palzkill, T (2005) Functional analysis of active site residues of the fosfomycin resistance enzyme FosA from Pseudomonas aeruginosa. J. Biol. Chem. 280 17786-91 PubMed
- ↑ Islam, MN et al. (2005) Construction of new forms of pyruvate carboxylase to assess the allosteric regulation by acetyl-CoA. Protein Eng. Des. Sel. 18 71-8 PubMed
- ↑ Nakamura, Y et al. (2005) A novel phosphatidylcholine-hydrolyzing phospholipase C induced by phosphate starvation in Arabidopsis. J. Biol. Chem. 280 7469-76 PubMed
- ↑ Rider, SD Jr et al. (2005) The protozoan parasite Cryptosporidium parvum possesses two functionally and evolutionarily divergent replication protein A large subunits. J. Biol. Chem. 280 31460-9 PubMed
- ↑ Skretas, G & Wood, DW (2005) Regulation of protein activity with small-molecule-controlled inteins. Protein Sci. 14 523-32 PubMed
- ↑ Cooper, KW & Baneyx, F (2001) Escherichia coli FtsH (HflB) degrades a membrane-associated TolAI-II-beta-lactamase fusion protein under highly denaturing conditions. Protein Expr. Purif. 21 323-32 PubMed
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgcgaccc atttgctgtc caccagtcat gctagccata
241 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc
301 acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc
361 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
421 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
481 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
541 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
601 gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct
661 ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac
721 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg
781 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg
841 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc
901 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga
961 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg
1021 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga
1081 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg
1141 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc
1201 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg
1261 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata
1321 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca
1381 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc
1441 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc
1501 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata
1561 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc
1621 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
1681 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
1741 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
1801 aacgcaatta atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg
1861 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
1921 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
1981 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta
2041 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc
2101 ggcgagaagc aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc
2161 ctgtcgttga ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca
2221 ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca
2281 acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc
2341 tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct
2401 acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc
2461 atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca
2521 gtaacccgta tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga
2581 aatccccctt acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc
2641 cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat
2701 gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc
2761 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
2821 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
2881 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
2941 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga
3001 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
3061 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
3121 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
3181 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
3241 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
3301 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
3361 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
3421 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
3481 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
3541 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
3601 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
3661 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
3721 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
3781 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
3841 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga acaataaaac
3901 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
3961 cttgctctag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
4021 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg
4081 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
4141 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
4201 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg
4261 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
4321 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
4381 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
4441 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
4501 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
4561 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
4621 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
4681 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
4741 agtttttcta agaattaatt catgagcgga tacatatttg aatgtattta gaaaaataaa
4801 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat
4861 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
4921 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
4981 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
5041 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
5101 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
5161 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
5221 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
5281 gcgccgctac agggcgcgtc ccattcgcca
