pET-23d(+)
At a Glance
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pET-23d(+), Image made with PlasMapper
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| Type |
Cloning, Expression |
|---|---|
| Origin of Replication |
pBR322, f1 |
| Copy # |
~40 |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 promoter |
| Accession Number |
|
| edit table |
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Notes
The pET-23a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. The map for pET-23d(+) is the same as pET-23a(+).
The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
pET-23d(+) is a 3663bp plasmid; the BamH I site is in the same reading frame as in pET-23c(+). An Nco I site is substituted for the Nde I site with a net 1bp deletion at position 238 of pET-23c(+)
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
References
- ↑ Fujita, K et al. (2005) Identification and molecular cloning of a novel glycoside hydrolase family of core 1 type O-glycan-specific endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum. J. Biol. Chem. 280 37415-22 PubMed
- ↑ Li, C et al. (2005) Two residues of rubisco activase involved in recognition of the Rubisco substrate. J. Biol. Chem. 280 24864-9 PubMed
- ↑ Liu, XW et al. (2005) Tissue inhibitor of metalloproteinase-1 protects human breast epithelial cells from extrinsic cell death: a potential oncogenic activity of tissue inhibitor of metalloproteinase-1. Cancer Res. 65 898-906 PubMed
- ↑ Dodd, RB et al. (2004) Solution structure of the Kaposi's sarcoma-associated herpesvirus K3 N-terminal domain reveals a Novel E2-binding C4HC3-type RING domain. J. Biol. Chem. 279 53840-7 PubMed
- ↑ Deveraux, QL et al. (1998) IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. EMBO J. 17 2215-23 PubMed
- ↑ Martineau, P et al. (1998) Expression of an antibody fragment at high levels in the bacterial cytoplasm. J. Mol. Biol. 280 117-27 PubMed
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatggt
241 atatctcctt cttaaagtta aacaaaatta tttctagagg gaaaccgttg tggtctccct
301 atagtgagtc gtattaattt cgcgggatcg agatctcggg cagcgttggg tcctggccac
361 gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
421 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
481 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
541 aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
601 ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
661 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
721 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
781 tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa
841 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
901 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
961 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
1021 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
1081 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
1141 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
1201 gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
1261 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
1321 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
1381 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
1441 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
1501 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
1561 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
1621 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
1681 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
1741 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
1801 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
1861 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
1921 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
1981 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
2041 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
2101 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
2161 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
2221 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
2281 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
2341 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
2401 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
2461 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
2521 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
2581 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
2641 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
2701 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
2761 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
2821 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
2881 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
2941 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
3001 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
3061 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
3121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
3181 cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
3241 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
3301 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
3361 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
3421 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
3481 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
3541 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
3601 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
3661 cca
