pET-23c(+)
At a Glance
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pET-23c(+), Image Provided by Novagen
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| Type |
Cloning, Expression |
|---|---|
| Origin of Replication |
pBR322, f1 |
| Copy # |
~40 |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 promoter |
| Accession Number |
|
| edit table |
</protect>
Notes
The pET-23a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. The map for pET-23c(+) is the same as pET-23a(+).
pET-23c(+) is a 3664bp plasmid.
The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
References
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg
241 tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt gtggtctccc
301 tatagtgagt cgtattaatt tcgcgggatc gagatctcgg gcagcgttgg gtcctggcca
361 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
421 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
481 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
541 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
601 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
661 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
721 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
781 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
841 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
901 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
961 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
1021 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
1081 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
1141 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
1201 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
1261 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
1321 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
1381 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
1441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
1501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
1561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
1621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
1681 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
1741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
1801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
1861 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
1921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
1981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
2041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
2101 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
2161 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
2221 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
2281 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
2341 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
2401 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
2461 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
2521 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
2581 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
2641 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
2701 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
2761 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
2821 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
2881 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
2941 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
3001 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
3061 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
3121 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
3181 ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
3241 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
3301 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
3361 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
3421 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
3481 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
3541 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
3601 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
3661 gcca
