pET-23b(+)
At a Glance
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pET-23b(+), Image made with PlasMapper
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| Type |
Cloning, Expression |
|---|---|
| Origin of Replication |
pBR322, f1 |
| Copy # |
~40 |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 promoter |
| Accession Number |
|
| edit table |
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Notes
The pET-23a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. The map for pET-23b(+) is the same as pET-23a(+).
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
References
[1] [2] [3] [4] [5] [6] [7] [8] [9]
- ↑ Heurgué-Hamard, V et al. (2005) The glutamine residue of the conserved GGQ motif in Saccharomyces cerevisiae release factor eRF1 is methylated by the product of the YDR140w gene. J. Biol. Chem. 280 2439-45 PubMed
- ↑ Karla, A et al. (2005) The identification of residues that control signal peptidase cleavage fidelity and substrate specificity. J. Biol. Chem. 280 6731-41 PubMed
- ↑ Li, L et al. (2005) Clarification of cinnamoyl co-enzyme A reductase catalysis in monolignol biosynthesis of Aspen. Plant Cell Physiol. 46 1073-82 PubMed
- ↑ Mata-Greenwood, E et al. (2005) Cyclic stretch increases VEGF expression in pulmonary arterial smooth muscle cells via TGF-beta1 and reactive oxygen species: a requirement for NAD(P)H oxidase. Am. J. Physiol. Lung Cell Mol. Physiol. 289 L288-9 PubMed
- ↑ Szweda, P et al. (2005) New effective sources of the Staphylococcus simulans lysostaphin. J. Biotechnol. 117 203-13 PubMed
- ↑ Gu, D et al. (2004) Identification and characterization of the DNA-binding domain of the multifunctional PutA flavoenzyme. J. Biol. Chem. 279 31171-6 PubMed
- ↑ Orelle, C et al. (2003) The conserved glutamate residue adjacent to the Walker-B motif is the catalytic base for ATP hydrolysis in the ATP-binding cassette transporter BmrA. J. Biol. Chem. 278 47002-8 PubMed
- ↑ Bassham, DC et al. (2000) AtVPS45 complex formation at the trans-Golgi network. Mol. Biol. Cell 11 2251-65 PubMed
- ↑ Dietrich, JB et al. (1991) Expression of mammalian tyrosine aminotransferase in Saccharomyces cerevisiae and Escherichia coli. Purification to homogeneity and characterization of the enzyme overproduced in the bacteria. Eur. J. Biochem. 201 399-407 PubMed
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat
241 gtatatctcc ttcttaaagt taaacaaaat tatttctaga gggaaaccgt tgtggtctcc
301 ctatagtgag tcgtattaat ttcgcgggat cgagatctcg ggcagcgttg ggtcctggcc
361 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct
421 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa
481 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct
541 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg
601 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt
661 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag
721 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg
781 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga
841 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
901 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
961 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
1021 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
1081 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
1141 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
1201 gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
1261 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
1321 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
1381 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
1441 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
1501 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
1561 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
1621 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
1681 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
1741 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
1801 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
1861 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
1921 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
1981 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
2041 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
2101 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
2161 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
2221 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
2281 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
2341 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
2401 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
2461 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
2521 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
2581 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
2641 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
2701 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
2761 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
2821 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
2881 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
2941 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
3001 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
3061 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
3121 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
3181 cccgaaaagt gccacctgaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt
3241 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
3301 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
3361 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
3421 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
3481 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
3541 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
3601 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
3661 cgcca
