pET-23a(+)
At a Glance
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pET-23a(+), Image made with PlasMapper
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| Type |
Cloning, Expression |
|---|---|
| Origin of Replication |
pBR322, f1 |
| Copy # |
~40 |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 promoter |
| Accession Number |
|
| edit table |
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Notes
The pET-23a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence.
The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
References
- ↑ Gallagher, CE et al. (2004) Gene duplication in the carotenoid biosynthetic pathway preceded evolution of the grasses. Plant Physiol. 135 1776-83 PubMed
- ↑ Wower, IK et al. (2004) Contributions of pseudoknots and protein SmpB to the structure and function of tmRNA in trans-translation. J. Biol. Chem. 279 54202-9 PubMed
- ↑ Marintchev, A et al. (2003) Site-directed mutagenesis analysis of the structural interaction of the single-strand-break repair protein, X-ray cross-complementing group 1, with DNA polymerase beta. Nucleic Acids Res. 31 580-8 PubMed
- ↑ Maeda, Y et al. (2002) Novel 33-kilodalton lipoprotein from Mycobacterium leprae. Infect. Immun. 70 4106-11 PubMed
- ↑ Zimmerman, KK et al. (1998) High-level expression of rat farnesyl:protein transferase in Escherichia coli as a translationally coupled heterodimer. Protein Expr. Purif. 14 395-402 PubMed
- ↑ Black, SM et al. (1997) Ventilation and oxygenation induce endothelial nitric oxide synthase gene expression in the lungs of fetal lambs. J. Clin. Invest. 100 1448-58 PubMed
- ↑ Shanley, TP et al. (1997) Requirement for C-X-C chemokines (macrophage inflammatory protein-2 and cytokine-induced neutrophil chemoattractant) in IgG immune complex-induced lung injury. J. Immunol. 158 3439-48 PubMed
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgcgaccc atttgctgtc caccagtcat gctagccata
241 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc
301 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gggcagcgtt gggtcctggc
361 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
421 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
481 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
541 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
601 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
661 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
721 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
781 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
841 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
901 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
961 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
1021 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
1081 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
1141 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
1201 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
1261 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
1321 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
1381 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
1441 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
1501 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
1561 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
1621 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
1681 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
1741 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
1801 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
1861 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
1921 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
1981 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
2041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
2101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
2161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
2221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
2281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
2341 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
2401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
2461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
2521 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
2581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
2641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
2701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
2761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
2821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
2881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
2941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
3001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
3061 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
3121 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
3181 ccccgaaaag tgccacctga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
3241 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
3301 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
3361 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
3421 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
3481 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
3541 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
3601 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
3661 tcgcca
