pET-22b(+)
At a Glance
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pET-22b(+), Image made with PlasMapper
<protect>
| Type |
Expression |
|---|---|
| Origin of Replication |
pBR322, f1 |
| Copy # |
~40 |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 promoter |
| Accession Number |
|
| edit table |
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Notes
The pET-22b(+) vector has an N-terminal pelB signal sequence for potential periplasmic localization, as well as optional C-terminal His•Tag sequence. The sequence is numbered by the pBR322 convention, thus the T7 expression region is reversed on the vector map.
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
References
- ↑ Seybert, A et al. (2002) Biochemical characterisation of the clamp/clamp loader proteins from the euryarchaeon Archaeoglobus fulgidus. Nucleic Acids Res. 30 4329-38 PubMed
- ↑ Barillà, D et al. (2001) Cleavage/polyadenylation factor IA associates with the carboxyl-terminal domain of RNA polymerase II in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 98 445-50 PubMed
- ↑ Koo, JH et al. (2000) The malonate decarboxylase operon of Acinetobacter calcoaceticus KCCM 40902 is regulated by malonate and the transcriptional repressor MdcY. J. Bacteriol. 182 6382-90 PubMed
- ↑ Lin, Z et al. () Functional expression of horseradish peroxidase in E. coli by directed evolution. Biotechnol. Prog. 15 467-71 PubMed
- ↑ MacBeath, G et al. (1998) Redesigning enzyme topology by directed evolution. Science 279 1958-61 PubMed
- ↑ MacKenzie-Graham, AJ et al. (1997) Myelin protein expression is increased in lymph nodes of mice with relapsing experimental autoimmune encephalomyelitis. J. Immunol. 159 4602-10 PubMed
- ↑ Lambalot, RH & Walsh, CT (1995) Cloning, overproduction, and characterization of the Escherichia coli holo-acyl carrier protein synthase. J. Biol. Chem. 270 24658-61 PubMed
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg
241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc
301 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc
361 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc
421 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
481 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
541 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
601 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
661 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat
721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
1021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
1081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
1141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
1201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
1261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
1321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
1381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
1441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
1501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
1561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
1621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
1681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
1741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
1861 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca
1921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
1981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
2041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
2101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
2161 aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga
2221 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
2281 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
2341 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta
2401 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
2461 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
2521 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
2581 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt
2641 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
2701 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
2761 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt
2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
2941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
3001 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac
3061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
3961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
4021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
4081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
4141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
4201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
4261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
4321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
4381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
4441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
4501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
4561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
4621 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
4681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
4741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
4801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
4861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
4921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
4981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt
5041 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
5101 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
5161 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
5221 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
5281 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
5341 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
5401 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
5461 aatgcgccgc tacagggcgc gtcccattcg cca
