pET-21d(+)
At a Glance

pET-21d(+), Image made with PlasMapper
<protect>
Type |
Expression |
---|---|
Origin of Replication |
pBR322, f1 |
Copy # |
~40 |
Markers |
Ampicillin |
Link to Sequence | |
Features |
T7 promoter
|
Accession Number |
|
edit table |
</protect>
Notes
The map for pET-21d(+) is the same as pET-21a(+) except that pET-21d(+) is a 5440bp plasmid. The BamH I site is in the same reading frame as in pET-21c(+). An Nco I site is in place of the Nde I site with a total 1bp deletion at position 238 of pET-21c(+). As a result, Nco I cuts pET21d(+) at 234, and Nhe I cuts at 229. For the remaining sites, subtract 3bp from each site beyond position 239 in pET-21a(+). Nde I does not cut pET-21d(+). Sty I is not unique in pET-21d(+).
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
References
- ↑ Kassavetis, GA et al. (2005) Reconfiguring the connectivity of a multiprotein complex: fusions of yeast TATA-binding protein with Brf1, and the function of transcription factor IIIB. Proc. Natl. Acad. Sci. U.S.A. 102 15406-11 PubMed
- ↑ Liu, G et al. (2005) High-quality homology models derived from NMR and X-ray structures of E. coli proteins YgdK and Suf E suggest that all members of the YgdK/Suf E protein family are enhancers of cysteine desulfurases. Protein Sci. 14 1597-608 PubMed
- ↑ Webb, BL et al. (1995) An interaction between the Escherichia coli RecF and RecR proteins dependent on ATP and double-stranded DNA. J. Biol. Chem. 270 31397-404 PubMed
Sequence
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatggt 241 atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca 301 attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg 361 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga 421 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt 481 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc 541 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca 601 ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc 661 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag 721 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 781 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg 841 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga 901 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta 961 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg 1021 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca 1081 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc 1141 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 1201 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc 1261 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat 1321 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt 1381 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg 1441 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg 1501 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc 1561 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 1621 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg 1681 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 1741 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 1801 gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat gcccttgaga 1861 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt 1921 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt 1981 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc 2041 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc 2101 gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat cgtgctcctg 2161 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg 2221 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca 2281 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc 2341 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca 2401 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc 2461 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta 2521 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat 2581 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc 2641 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa 2701 caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc 2761 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 2821 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 2881 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc 2941 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc ggtgtgaaat 3001 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac 3061 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 3121 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 3181 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 3241 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 3301 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 3361 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 3421 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 3481 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 3541 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 3601 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 3661 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 3721 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 3781 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 3841 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 3901 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 3961 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 4021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 4081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 4141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 4201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 4261 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc 4321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 4381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 4441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 4501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 4561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 4621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 4681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 4741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 4801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 4861 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 4921 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt 4981 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 5041 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 5101 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 5161 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 5221 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 5281 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 5341 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 5401 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca