pET-19b
At a Glance

pET-19b, Image made with PlasMapper
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Type |
Cloning, Expression |
---|---|
Origin of Replication |
pBR322 |
Copy # |
~40 |
Markers |
Ampicillin |
Link to Sequence | |
Features |
T7 promoter |
Accession Number |
|
edit table |
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Notes
The pET-19b vector includes an N-terminal His•Tag sequence, an enterokinase cleavage site and three cloning sites.
History
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Sources
References
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Sequence
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 301 cgggctttgt tagcagccgg atcctcgagc atatgcttgt cgtcgtcgtc gatatggccg 361 ctgctgtgat gatgatgatg atgatgatga tgatggccca tggtatatct ccttcttaaa 421 gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag 481 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc 541 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa 601 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc 661 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg 721 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga 781 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata 841 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt 901 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca 961 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 1021 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 1081 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 1141 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc 1201 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga 1261 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga 1321 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 1381 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt 1441 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 1501 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 1561 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat 1621 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc 1681 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat 1741 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca 1801 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 1861 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 1921 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt 1981 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca 2041 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta 2101 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct 2161 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc 2221 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta 2281 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct 2341 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc 2401 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg 2461 cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg 2521 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct 2581 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg 2641 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag 2701 aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag 2761 cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt 2821 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga 2881 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc 2941 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc 3001 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac 3061 acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg 3121 ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc 3181 atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa 3241 cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat 3301 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc 3361 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag 3421 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 3481 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 3541 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 3601 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatatgcggt 3661 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 3721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 3781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 3841 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3901 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3961 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 4021 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 4081 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 4141 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 4201 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 4261 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 4321 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 4381 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 4441 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 4501 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 4561 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 4621 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 4681 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 4741 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 4801 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 4861 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 4921 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt 4981 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 5041 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 5101 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 5161 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 5221 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 5281 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 5341 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 5401 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 5461 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 5521 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 5581 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 5641 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 5701 cctttcgtct tcaagaa
- ↑ Angelini, S et al. (2005) FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. EMBO Rep. 6 476-81 PubMed
- ↑ Kim, J et al. (2005) Adenylate kinase of Escherichia coli, a component of the phage T4 dNTP synthetase complex. J. Biol. Chem. 280 28221-9 PubMed