pET-17b
At a Glance
pET-17b, Image made with PlasMapper
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| Type |
Cloning |
|---|---|
| Origin of Replication |
pBR322 |
| Copy # |
~40 |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 promoter |
| Accession Number |
|
| edit table |
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Notes
The pET-17b vector includes an N-terminal 11aa T7•Tag sequence and a region of useful cloning sites. Included in the multiple cloning region are two BstX I sites, which allow efficient cloning using an asymmetric linker. Unique sites are shown on the vector map except for the two BstX I sites.
History
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Sources
References
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Sequence
1 ttatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc
61 aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc
121 tttgttagca gccggatctg ctcgagcggc cgccagtgtg atggatatct gcagaattcc
181 agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttga atcccgaccc
241 atttgctgtc caccagtcat gctagccata tgtatatctc cttcttaaag ttaaacaaaa
301 ttatttctag agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga
361 tcgagatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
421 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
481 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
541 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
601 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
661 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
721 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
781 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
841 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
901 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
961 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
1021 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
1081 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
1141 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
1201 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg
1261 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
1321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
1381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
1441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
1501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
1561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
1621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
1681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
1741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
1801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
1861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
1921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
1981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
2041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
2101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
2161 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
2221 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
2281 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
2341 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
2401 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
2461 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
2521 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
2581 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
2641 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
2701 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
2761 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
2821 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
2881 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
2941 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
3001 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
3061 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
3121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
3181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
3241 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
3301 caagaa
