pCOLADuet-1
At a Glance
pCOLADuet-1, Image made with PlasMapper
<protect>
| Type |
Coexpression |
|---|---|
| Origin of Replication |
ColA |
| Copy # | |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 Promoter His Tag S Tag 2 MCS lacI RBS |
| Accession Number |
|
| edit table |
</protect>
Notes
pCOLADuet™-1 is designed for the coexpression of two target genes from a single plasmid. The vector has the COLA replicon from ColA[1]. This vector can be transformed into the same cell with plasmids containing compatible origins of replication and drug resistance genes for coexpression of up to eight target genes[2][3]. Plasmids that share common origins of replication or antibiotic resistance genes should not be used for the coexpression of two proteins[4].
History
pCOLADuet-1 for coexpression was created in order to provide a useful alternative to the complicated and expensive expression systems, such as yeast, baculovirus or mammalian cell culture, which are commonly used to overcome problems of heterologous protein expression.
Sources
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Zverev, VV & Khmel', IA (1985) [Nucleotide sequence of the replicative region of the plasmid ColA-CA31]. Mol. Gen. Mikrobiol. Virusol. 18-24 PubMed
- ↑ Novy, R., et all. 2002. inNovations 15,2.
- ↑ Held, D., et al. 2003. inNovations 18, 4.
- ↑ Tolia, NH & Joshua-Tor, L (2006) Strategies for protein coexpression in Escherichia coli. Nat. Methods 3 55-64 PubMed
Sequence
1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg 481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg 541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt 601 aacgaccctg ccctgaaccg acgacaagct gacgaccggg tctccgcaag tggcactttt 661 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 721 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt 781 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa 841 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg 901 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa 961 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca 1021 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc 1081 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cggtcgctgt taaaaggaca 1141 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt 1201 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt 1261 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat 1321 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc 1381 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt 1441 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat 1501 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt 1561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 1621 tgaatgtatt tagaaaaata aacaaatagg catgctagcg cagaaacgtc ctagaagatg 1681 ccaggaggat acttagcaga gagacaataa ggccggagcg aagccgtttt tccataggct 1741 ccgcccccct gacgaacatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac 1801 aggactataa agataccagg cgtttccccc tgatggctcc ctcttgcgct ctcctgttcc 1861 cgtcctgcgg cgtccgtgtt gtggtggagg ctttacccaa atcaccacgt cccgttccgt 1921 gtagacagtt cgctccaagc tgggctgtgt gcaagaaccc cccgttcagc ccgactgctg 1981 cgccttatcc ggtaactatc atcttgagtc caacccggaa agacacgaca aaacgccact 2041 ggcagcagcc attggtaact gagaattagt ggatttagat atcgagagtc ttgaagtggt 2101 ggcctaacag aggctacact gaaaggacag tatttggtat ctgcgctcca ctaaagccag 2161 ttaccaggtt aagcagttcc ccaactgact taaccttcga tcaaaccgcc tccccaggcg 2221 gttttttcgt ttacagagca ggagattacg acgatcgtaa aaggatctca agaagatcct 2281 ttacggattc ccgacaccat cactctagat ttcagtgcaa tttatctctt caaatgtagc 2341 acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc 2401 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc 2461 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 2521 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 2581 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt 2641 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg 2701 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc 2761 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat 2821 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt 2881 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc 2941 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc 3001 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag 3061 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt 3121 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat 3181 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag 3241 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac 3301 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg 3361 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg 3421 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt 3481 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc 3541 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact 3601 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg 3661 gatctcgacg ctctccctta tgcgactcct gcattaggaa attaatacga ctcactata
