pCDFDuet-1
At a Glance
pCDFDuet-1, Image made with PlasMapper
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| Type |
Coexpression |
|---|---|
| Origin of Replication |
CDF |
| Copy # | |
| Markers |
Streptomycin/ Spectinomycin |
| Link to Sequence | |
| Features |
2 T7 Promoters 2 lac Operators His Tag S Tag 2 MCS RBS lacI coding seqence |
| Accession Number |
|
| edit table |
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Notes
pCDFDuet-1 is designed for the coexpression of two target ORFs. It encodes two MCS each of which is preceded by a T7 promoter, lac operator, and rbs. The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. It can be used in combination with pACYCDuet-1, pRSFDuet-1, and pETDuet-1 in an appropriate host strain for the coexpression of 4 to 8 target proteins. Plasmids that share common origins of replication or antibiotic resistance genes should not be used for the coexpression of two proteins[1].
History
The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli to be used together or with existing pET, pCDF, pRSF, and pETcoco recombinants with compatible replicons and antibiotic resistance to coexpress up to eight proteins in the same cell[2][3].
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg 481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg 541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt 601 aacgaccctg ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc 661 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt 721 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat 781 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc 841 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg 901 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg 961 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct 1021 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca 1081 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt 1141 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa 1201 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca 1261 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca 1321 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta 1381 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag 1441 tcgatacttc ggcgatcacc gcttccctca tactcttcct ttttcaatat tattgaagca 1501 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 1561 aaatagctag ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga 1621 cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa 1681 cagcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat 1741 aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag 1801 tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt cgctccctct 1861 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt tctcccttac 1921 gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt gtaggtcgtt 1981 cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc 2041 ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact ggcagcagcc 2101 attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt 2161 gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc 2221 agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag 2281 gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat 2341 cctttgatct tttctactga accgctctag atttcagtgc aatttatctc ttcaaatgta 2401 gcacctgaag tcagccccat acgatataag ttgtaattct catgttagtc atgccccgcg 2461 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg 2521 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg 2581 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 2641 gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc 2701 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg 2761 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg 2821 tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc 2881 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca 2941 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc 3001 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc 3061 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc 3121 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt 3181 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca 3241 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga 3301 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc 3361 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg 3421 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt 3481 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc 3541 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca 3601 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga 3661 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc 3721 gggatctcga cgctctccct tatgcgactc ctgcattagg aaattaatac gactcactat 3781 a
