pCDF-2 Ek/LIC
At a Glance
pCDF-2 Ek/LIC, Image made with PlasMapper
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| Type |
Cloning, Expression |
|---|---|
| Origin of Replication |
CDF |
| Copy # |
High-level |
| Markers |
Streptomycin/Spectinomycin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number |
|
| edit table |
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Notes
The pCDF-2 Ek/LIC Vector is a LIC-compatible derivative of pCDF-1b. This plasmid is compatible with the LIC Duet Adaptors for coexpression of two proteins as well as with pET-46 and pRSF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification [1].
History
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Sources
References
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Sequence
1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
61 gagatatacc atggcacatc accaccacca tcacgtggat gacgacgaca agagtccggg
121 cttctcctca aatctcgagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca
181 catggactcg tctactagcg cagcttaatt aacctaggct gctgccaccg ctgagcaata
241 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca
301 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
361 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt catcgtggcc
421 ggatcttgcg gcccctcggc ttgaacgaat tgttagacat tatttgccga ctaccttggt
481 gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
541 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
601 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
661 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
721 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
781 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
841 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
901 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
961 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
1021 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
1081 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
1141 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
1201 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca tactcttcct
1261 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
1321 atgtatttag aaaaataaac aaatagctag ctcactcggt cgctacgctc cgggcgtgag
1381 actgcggcgg gcgctgcgga cacatacaaa gttacccaca gattccgtgg ataagcaggg
1441 gactaacatg tgaggcaaaa cagcagggcc gcgccggtgg cgtttttcca taggctccgc
1501 cctcctgcca gagttcacat aaacagacgc ttttccggtg catctgtggg agccgtgagg
1561 ctcaaccatg aatctgacag tacgggcgaa acccgacagg acttaaagat ccccaccgtt
1621 tccggcgggt cgctccctct tgcgctctcc tgttccgacc ctgccgttta ccggatacct
1681 gttccgcctt tctcccttac gggaagtgtg gcgctttctc atagctcaca cactggtatc
1741 tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc
1801 ccgactgctg cgccttatcc ggtaactgtt cacttgagtc caacccggaa aagcacggta
1861 aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa
1921 cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt
1981 tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt
2041 cgatcaaacc acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa
2101 aaaaaggatc tcaagaagat cctttgatct tttctactga accgctctag atttcagtgc
2161 aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtaattct
2221 catgttagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
2281 catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
2341 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
2401 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
2461 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
2521 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
2581 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc
2641 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
2701 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
2761 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
2821 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
2881 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
2941 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
3001 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
3061 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
3121 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
3181 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
3241 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
3301 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
3361 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
3421 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
3481 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
3541 aaattaatac gactcactat a
