pBEn-SBP-SET1a
At a Glance
pBEn-SBP-SET1a Image provided by Agilent-Genomics
<protect>
| Type |
Expression |
|---|---|
| Origin of Replication |
pBR322 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
P T7/lacO |
| Accession Number |
|
| edit table |
</protect>
Notes
The pBEn vectors are based on the pET-11a vector and contain the tag coding sequence(s) inserted upstream of a multiple cloning site (MCS) to allow for fusion of the tag(s) at the N-terminus of the cloned protein-coding sequence. The efficient translation of the tags in E. coli ensures that fusion proteins containing the tags at the N terminus will be consistently expressed at high levels.
History
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Sources
References
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Sequence
1 TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA 51 GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGACCCCGAA 101 GAGGCGAGTG TTACTTCAAC AGAAGAAACC TTAACGCCAG CACAGGAGGC 151 CGCACGCACC CGCGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG 201 CTGCCACCGC TGAACAAACT AGTGACGAGA AGACCACCGG CTGGCGGGGC 251 GGCCACGTGG TGGAGGGCCT GGCCGGCGAG CTGGAGCAGC TGCGGGCCAG 301 GCTGGAGCAC CACCCTCAGG GCCAGCGGGA GCCCTCCGGC GGCTGCAAGC 351 TGGGCCTGCT GGTTCCGCGT GGATCTGGAT CCGAATTCTC TTCCCGGGTC 401 TTGTTCCATG GGTCGACTCG AGCTCAAGCT TAGATCCGGC TGCTAACAAA 451 GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC 501 ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG 551 GAGGAACTAT ATCCGGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA 601 CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG 651 AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA 701 CTGTGATAAA CTACCGCATT AAAGCTAATC GAAGATAAGC TGTCAAACAT 751 GACAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT 801 TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG 851 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT 901 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG 951 AAAAAGGAAC AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT 1001 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG 1051 AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA 1101 ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC 1151 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA 1201 TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC 1251 TCAGAATGAC TTGGTTGACG CGTCACCAGT CACAGAAAAG CATCTTACGG 1301 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT 1351 AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT 1401 AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT 1451 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG 1501 ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT 1551 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA 1601 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT 1651 GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC 1701 ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 1751 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT 1801 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT 1851 ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA 1901 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG 1951 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 2001 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 2051 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG 2101 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT 2151 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT 2201 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG 2251 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 2301 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 2351 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC 2401 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 2451 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC 2501 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG 2551 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 2601 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC 2651 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA 2701 GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG 2751 CGAGGAAGCG GAAGACCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT 2801 GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG 2851 ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG 2901 TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG 2951 GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG 3001 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC 3051 AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT 3101 GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT 3151 CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG 3201 TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA 3251 CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA 3301 TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC 3351 GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT 3401 ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT 3451 CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG 3501 AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT 3551 TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG 3601 CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA 3651 GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC 3701 CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG 3751 GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC 3801 TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG 3851 AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG 3901 TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA 3951 GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC 4001 TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT 4051 ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA 4101 AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG 4151 CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC 4201 TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC 4251 GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG 4301 CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC 4351 ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC 4401 GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC 4451 TCAAGGGCAT CGGTCGAGAT CCCGGTGCCT AATGAGTGAG CTAACTTACA 4501 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 4551 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 4601 TTGGGCGCCA GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG 4651 ATTGCCCTTC ACCGCCTGGC CCTGAGAGAG TTGCAGCAAG CGGTCCACGC 4701 TGGTTTGCCC CAGCAGGCGA AAATCCTGTT TGATGGTGGT TAACGGCGGG 4751 ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA CCGAGATATC 4801 CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG 4851 CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC 4901 AGCATTTGCA TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC 4951 CCGTTCCGCT ATCGGCTGAA TTTGATTGCG AGTGAGATAT TTATGCCAGC 5001 CAGCCAGACG CAGACGCGCC GAGACAGAAC TTAATGGGCC CGCTAACAGC 5051 GCGATTTGCT GGTGACCCAA TGCGACCAGA TGCTCCACGC CCAGTCGCGT 5101 ACCGTCTTCA TGGGAGAAAA TAATACTGTT GATGGGTGTC TGGTCAGAGA 5151 CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC CACAGCAATG 5201 GCATCCTGGT CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG 5251 CGCGAGAAGA TTGTGCACCG CCGCTTTACA GGCTTCGACG CCGCTTCGTT 5301 CTACCATCGA CACCACCACG CTGGCACCCA GTTGATCGGC GCGAGATTTA 5351 ATCGCCGCGA CAATTTGCGA CGGCGCGTGC AGGGCCAGAC TGGAGGTGGC 5401 AACGCCAATC AGCAACGACT GTTTGCCCGC CAGTTGTTGT GCCACGCGGT 5451 TGGGAATGTA ATTCAGCTCC GCCATCGCCG CTTCCACTTT TTCCCGCGTT 5501 TTCGCAGAAA CGTGGCTGGC CTGGTTCACC ACGCGGGAAA CGGTCTGATA 5551 AGAGACACCG GCATACTCTG CGACATCGTA TAACGTTACT GGTTTCACAT 5601 TCACCACCCT GAATTGACTG TCTTCCGGGC GCTATCATGC CATACCGCGA 5651 AAGGTTTTGC GCCATTCGAT GGTGTCCGGG ATCTCGACGC TCTCCCTTAT 5701 GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC 5751 ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC 5801 CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA 5851 GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG 5901 GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC 5951 GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT
