pBEc-SBP-SET3-Q

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At a Glance

pBEc-SBP-SET3-Q, Image Provided by Agilent-Genomics

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Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent-Genomics

Features

T7 Promoter
lac operator
extended RBS
MCS
SBP Tag
SET3 Tag
Q Tag
lacI repressor ORF

Accession Number


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Notes

This plasmid has been discontinued by Stratagene.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Agilent-Genomics. No longer Found

References

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Sequence

   1  TTAATACGAC TCACTATAGG GGAATTGTGA GCGGATAACA ATTCCCCTCT  
  51  AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACCAT GGCTAGCATG  
 101  ACTGGTGGAC AGCAAATGGG TCGGATCCAT GTATCCACGT GGGAATGGTA  
 151  CCGACGAGAA GACCACCGGC TGGCGGGGCG GCCACGTGGT GGAGGGCCTG  
 201  GCCGGCGAGC TGGAGCAGCT GCGGGCCAGG CTGGAGCACC ACCCTCAGGG  
 251  CCAGCGGGAG CCCTCCGGCG GCTGCAAGCT GGGCACTAGT ATGGACCCCG  
 301  AAGAGGCGAG TGTTACTTCA ACAGAAGAAA CCTTAACGCC AGCACAGGAG  
 351  GCCGCAGAGA CCGAAGCTGC TAACAAAGCC GAAGAGGAAG CTGAGTTGGA  
 401  GGCTGAAACC GCTGAACAAG CGCGCATGAG CTCCAATTCA CTGGCCGTCG  
 451  TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC  
 501  CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG  
 551  CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATAAGCTG  
 601  AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT  
 651  TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCCGCAA GAGGCCCGGC  
 701  AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC  
 751  GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC  
 801  GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA  
 851  AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC  
 901  TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT  
 951  GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA  
1001  AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC  
1051  TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC  
1101  GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC  
1151  AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG  
1201  TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT  
1251  CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG  
1301  TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC  
1351  GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA  
1401  AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT  
1451  AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG  
1501  GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT  
1551  CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA  
1601  GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT  
1651  TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG  
1701  ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC  
1751  TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG  
1801  GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT  
1851  ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT  
1901  CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG  
1951  TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA  
2001  AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA  
2051  ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG  
2101  GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA  
2151  AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC  
2201  AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA  
2251  CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA  
2301  GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC  
2351  CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC  
2401  CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC  
2451  AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT  
2501  ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG  
2551  TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA  
2601  AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA  
2651  GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG  
2701  CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT  
2751  CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC  
2801  CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA  
2851  GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC  
2901  CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT  
2951  ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG  
3001  CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA  
3051  CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG  
3101  TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG  
3151  AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA  
3201  TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA  
3251  GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT  
3301  TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT  
3351  GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA  
3401  CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG  
3451  GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA  
3501  GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC  
3551  CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT  
3601  TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA  
3651  GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG  
3701  GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC  
3751  CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT  
3801  GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA  
3851  TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA  
3901  TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT  
3951  TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG  
4001  GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC  
4051  ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG  
4101  ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG  
4151  ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC  
4201  CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT  
4251  CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC  
4301  GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA  
4351  CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG  
4401  CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA  
4451  GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA  
4501  TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT  
4551  GGGTTGAAGG CTCTCAAGGG CATCGGTCGA GATCCCGGTG CCTAATGAGT  
4601  GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG  
4651  GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA  
4701  GGCGGTTTGC GTATTGGGCG CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA  
4751  CGGGCAACAG CTGATTGCCC TTCACCGCCT GGCCCTGAGA GAGTTGCAGC  
4801  AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT GTTTGATGGT  
4851  GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA  
4901  CTACCGAGAT ATCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC  
4951  ATTGCGCCCA GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC  
5001  GATGCCCTCA TTCAGCATTT GCATGGTTTG TTGAAAACCG GACATGGCAC  
5051  TCCAGTCGCC TTCCCGTTCC GCTATCGGCT GAATTTGATT GCGAGTGAGA  
5101  TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG AACTTAATGG  
5151  GCCCGCTAAC AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA  
5201  CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT  
5251  GTCTGGTCAG AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC  
5301  TTCCACAGCA ATGGCATCCT GGTCATCCAG CGGATAGTTA ATGATCAGCC  
5351  CACTGACGCG TTGCGCGAGA AGATTGTGCA CCGCCGCTTT ACAGGCTTCG  
5401  ACGCCGCTTC GTTCTACCAT CGACACCACC ACGCTGGCAC CCAGTTGATC  
5451  GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGGCCA  
5501  GACTGGAGGT GGCAACGCCA ATCAGCAACG ACTGTTTGCC CGCCAGTTGT  
5551  TGTGCCACGC GGTTGGGAAT GTAATTCAGC TCCGCCATCG CCGCTTCCAC  
5601  TTTTTCCCGC GTTTTCGCAG AAACGTGGCT GGCCTGGTTC ACCACGCGGG  
5651  AAACGGTCTG ATAAGAGACA CCGGCATACT CTGCGACATC GTATAACGTT  
5701  ACTGGTTTCA CATTCACCAC CCTGAATTGA CTCTCTTCCG GGCGCTATCA  
5751  TGCCATACCG CGAAAGGTTT TGCGCCATTC GATGGTGTCC GGGATCTCGA  
5801  CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT  
5851  GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG  
5901  CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA  
5951  CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT  
6001  GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG  
6051  CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAA