pBAC-2cp
At a Glance
pBAC-2cp, Image made with PlasMapper
<protect>
| Type |
Cloning, Expression |
|---|---|
| Origin of Replication |
f1 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
polh Promoter |
| Accession Number |
|
| edit table |
</protect>
Notes
The pBAC-2cp Ek/LIC vector is an Ek/LIC version of pBAC-2cp. Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions[1][2]. The pBAC-2cp Ek/LIC vector is a baculovirus transfer plasmid designed for simplified cloning and expression of target genes in insect cells. A Ligation-Independent Cloning (LIC) version of the vector is available for rapid, directional cloning of PCR products adjacent to the enterokinase cleavage site.
History
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Sources
References
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Sequence
1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag
61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac
121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat
181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg
241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac
301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat
361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac
421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat
481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa
541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac
601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt
661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt
721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt
781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat
841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt
901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa
961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt
1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt
1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta
1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aaccatctcg
1201 caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata
1261 tgcccatggt ccatcatcac caccatcact ccgcgggttt ggtgcccaga ggcagcggca
1321 aagaaacggc ggcggcgaaa tttgaacgcc aacacatgga cagcgctagc ggtggaggtg
1381 atgacgacga caagagcccg ggcttctcct caaaaggcct ggatccgaat tcgagctcga
1441 agcttgcggc cgcactcgag caccaccacc accaccacta acctaggtag ctgagcgcat
1501 gcaagctgat ccgggttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt
1561 tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta
1621 tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc
1681 ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc
1741 gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag
1801 cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc
1861 gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact
1921 cgacgtaaac acgttaaata gagcttggac atatttaaca tcgggcgtgt tagctttatt
1981 aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt
2041 tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt
2101 agttgagctt tttggaatta tttctgattg cgggcgtttt tgggcgggtt tcaatctaac
2161 tgtgcccgat tttaattcag acaacacgtt agaaagcgat ggtgcaggcg gtggtaacat
2221 ttcagacggc aaatctacta atggcggcgg tggtggagct gatgataaat ctaccatcgg
2281 tggaggcgca ggcggggctg gcggcggagg cggaggcgga ggtggtggcg gtgatgcaga
2341 cggcggttta ggctcaaatg tctctttagg caacacagtc ggcacctcaa ctattgtact
2401 ggtttcgggc gccgtttttg gtttgaccgg tctgagacga gtgcgatttt tttcgtttct
2461 aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag gttccgtcgg
2521 cattggtgga gcgggcggca attcagacat cgatggtggt ggtggtggtg gaggcgctgg
2581 aatgttaggc acgggagaag gtggtggcgg cggtgccgcc ggtataattt gttctggttt
2641 agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc gctggctgca caacggaagg
2701 tcgtctgctt cgaggcagcg cttggggtgg tggcaattca atattataat tggaatacaa
2761 atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg tttaccgtgc cgatatttaa
2821 caaccgctca atgtaagcaa ttgtattgta aagagattgt ctcaagctcg gatcgatccc
2881 gcacgccgat aacaagcctt ttcattttta ctacagcatt gtagtggcga gacacttcgc
2941 tgtcgtcgag gtttaaacgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
3001 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
3061 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
3121 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
3181 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
3241 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
3301 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
3361 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
3421 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
3481 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
3541 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
3601 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
3661 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
3721 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
3781 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
3841 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
3901 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
3961 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
4021 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
4081 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
4141 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
4201 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
4261 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
4321 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
4381 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
4441 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
4501 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
4561 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
4621 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
4681 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
4741 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
4801 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
4861 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
4921 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
4981 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
5041 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
5101 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
5161 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
5221 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
5281 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
5341 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
5401 ctattacgcc a
