SG4044
| Name | SG4044 |
| Synonyms | |
| Genotype | lon-100 Δ(gal-pgl)324 lac thi rpsL |
| Phenotype | Streptomycin resistant, non-mucoid, Lon- |
| Sources | LMBP 670 |
| References | PMID:7026534 PMID:3888955 |
Notes
The Lon protease plays a major role in the proteolytic machinery of E. coli. Lon mutants are highly pleioptropic and produce mucoid colonies that are difficult to handle. The (gal-pgl) deletion eliminates mucoidy and restores normal colony morphology.
Lon mutants may be useful in stabilizing heterologous proteins expressed in E. coli (see e.g. Remaut et al., Nucleic Acids Res. 11 (1983), 4677-4688).
