PinPoint Xa-3
At a Glance
<protect>
| Type |
Expression |
|---|---|
| Origin of Replication | |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number | |
| edit table |
</protect>
Notes
The PinPoint Xa Protein Purification System is used for the production and purification of fusion proteins that are biotinylated in vivo. The PinPoint Vectors an encoded endoproteinase Factor Xa proteolytic site that provides a way to separate the purification tag from the native protein. These vectors also carry a multiple cloning region for use in the construction of fusion proteins. The PinPoint Xa Vector contains the chloramphenicol acetyltransferase gene and is provided as a means of monitoring protein expression, purification and processing conditions.
The PinPoint™ Xa-3 Vector sequence is identical to PinPoint™ Xa-1 Vector, except for two additional adenosines at position 394.
History
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Sources
Promega. Discontinued.
References
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Sequence
1 ATGAAACTGA AGGTAACAGT CAACGGCACT GCGTATGACG TTGACGTTGA 51 CGTCGACAAG TCACACGAAA ACCCGATGGG CACCATCCTG TTCGGCGGCG 101 GCACCGGCGG CGCGCCGGCA CCGGCAGCAG GTGGCGCAGG CGCCGGTAAG 151 GCCGGAGAGG GCGAGATTCC CGCTCCGCTG GCCGGCACCG TCTCCAAGAT 201 CCTCGTGAAG GAGGGTGACA CGGTCAAGGC TGGTCAGACC GTGCTCGTTC 251 TCGAGGCCAT GAAGATGGAG ACCGAGATCA ACGCTCCCAC CGACGGCAAG 301 GTCGAGAAGG TCCTGGTCAA GGAGCGTGAC GCGGTGCAGG GCGGTCAGGG 351 TCTCATCAAG ATCGGGGATC TCGAGCTCAT CGAAGGTCGC GAAAAGCTTC 401 AGCTGGGATC CGGTACCGAT ATCAGATCTC CCGGGGCGGC CGCGATCTGG 451 TTCTATAGTG TCACCTAAAT CGTATGTGTA TGATACATAA GGTTATGTAT 501 TAATTGTAGC CGCGTTCTAA CGACAATATG TCCATATGGT GCACTCTCAG 551 TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA 601 CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC 651 AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC 701 GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT 751 TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT 801 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA 851 TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 901 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 951 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC 1001 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT 1051 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC 1101 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC 1151 GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 1201 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 1251 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT 1301 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA 1351 AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT 1401 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA 1451 CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 1501 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 1551 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG 1601 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA 1651 TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC 1701 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA 1751 GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 1801 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC 1851 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA 1901 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT 1951 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA 2001 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 2051 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC 2101 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG 2151 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG 2201 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA 2251 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG 2301 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA 2351 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 2401 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC 2451 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG 2501 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA 2551 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG 2601 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT 2651 TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 2701 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC 2751 GCGCGTTGGC CGATTCATTA ATGCAGGTTA ACCTGGCTTA TCGAAATTAA 2801 TACGACTCAC TATAGGGAGA CCGGCCTCGA GCAGCAAGGA GATGGCGCCC 2851 AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC 2901 GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG 2951 CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG 3001 ATGCGTCCGG CGTAGAGGAT CGATCCGGGC TTATCGACTG CACGGTGCAC 3051 CAATGCTTCT GGCGTCAGGC AGCCATCGGA AGCTGTGGTA TGGCTGTGCA 3101 GGTCGTAAAT CACTGCATAA TTCGTGTCGC TCAAGGCGCA CTCCCGTTCT 3151 GGATAATGTT TTTTGCGCCG ACATCATAAC GGTTCTGGCA AATATTCTGA 3201 AATGAGCTGT TGACAATTAA TCATCGGCTC GTATAATGTG TGGAATTGTG 3251 AGCGGATAAC AATTTCACAC AGGAAACAGA ATTCCCAGCT TGGCTGCAGA 3301 ACCATTCCAT TCGTTGATCC GGGAGTAACT CAC
